ENSG00000109466

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000226725 ENSG00000109466 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL2 protein_coding protein_coding 4.07412 2.215589 5.608198 0.2804968 0.2824295 1.33592 2.1428175 0.3414581 3.7949540 0.17919420 0.4815426 3.4364544 0.45354167 0.1774667 0.67860000 0.50113333 0.10006350 0.04945214 FALSE TRUE
ENST00000421009 ENSG00000109466 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL2 protein_coding protein_coding 4.07412 2.215589 5.608198 0.2804968 0.2824295 1.33592 0.3728769 0.0000000 0.4254520 0.00000000 0.3431299 5.4444418 0.11677083 0.0000000 0.07723333 0.07723333 0.45834547 0.04945214 FALSE TRUE
ENST00000506761 ENSG00000109466 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL2 protein_coding protein_coding 4.07412 2.215589 5.608198 0.2804968 0.2824295 1.33592 0.1440236 0.5399928 0.0000000 0.27812808 0.0000000 -5.7813408 0.07460000 0.2161333 0.00000000 -0.21613333 0.04945214 0.04945214 FALSE TRUE
ENST00000506824 ENSG00000109466 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL2 protein_coding nonsense_mediated_decay 4.07412 2.215589 5.608198 0.2804968 0.2824295 1.33592 0.3007835 0.4743940 0.1654857 0.05173442 0.1654857 -1.4648271 0.08335417 0.2209667 0.02706667 -0.19390000 0.05279825 0.04945214 FALSE TRUE
MSTRG.25690.5 ENSG00000109466 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL2 protein_coding   4.07412 2.215589 5.608198 0.2804968 0.2824295 1.33592 0.6803692 0.8239053 0.9158698 0.20887074 0.2670606 0.1509258 0.17819167 0.3637667 0.16053333 -0.20323333 0.11888358 0.04945214 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000109466 E001 0.2924217 0.0290785164 6.373532e-01   4 165207561 165207569 9 + 0.145 0.000 -10.887
ENSG00000109466 E002 0.4396707 0.0282087078 3.753215e-01 5.197475e-01 4 165207570 165207590 21 + 0.202 0.000 -11.470
ENSG00000109466 E003 7.2771082 0.0022721774 6.994019e-04 2.896742e-03 4 165207591 165207822 232 + 0.995 0.498 -2.051
ENSG00000109466 E004 9.6957743 0.0417873470 3.370548e-03 1.135223e-02 4 165207823 165207902 80 + 1.102 0.623 -1.862
ENSG00000109466 E005 0.1515154 0.0442318016 1.000000e+00   4 165209400 165209462 63 + 0.078 0.000 -9.886
ENSG00000109466 E006 0.0000000       4 165209893 165210018 126 +      
ENSG00000109466 E007 0.0000000       4 165210019 165210035 17 +      
ENSG00000109466 E008 0.1451727 0.0428732826 1.000000e+00   4 165210036 165210180 145 + 0.078 0.000 -9.885
ENSG00000109466 E009 0.5954526 0.0193874923 2.343979e-01 3.676296e-01 4 165219644 165219722 79 + 0.253 0.000 -11.886
ENSG00000109466 E010 12.6852526 0.0148987517 4.716691e-03 1.514206e-02 4 165219934 165220000 67 + 1.188 0.838 -1.290
ENSG00000109466 E011 15.3684490 0.0013893765 8.195222e-02 1.626143e-01 4 165220001 165220059 59 + 1.225 1.069 -0.558
ENSG00000109466 E012 0.3268771 0.0323467416 4.747610e-01   4 165223938 165224057 120 + 0.078 0.187 1.443
ENSG00000109466 E013 16.3755840 0.0011141090 1.072472e-01 2.014581e-01 4 165228807 165228913 107 + 1.245 1.107 -0.491
ENSG00000109466 E014 0.2214452 0.0387969004 1.781232e-01   4 165238776 165238777 2 + 0.000 0.187 12.498
ENSG00000109466 E015 23.0381282 0.0009006728 4.831029e-01 6.211814e-01 4 165238778 165238899 122 + 1.360 1.319 -0.143
ENSG00000109466 E016 0.0000000       4 165240348 165240464 117 +      
ENSG00000109466 E017 19.7368941 0.0009544913 3.100295e-02 7.392036e-02 4 165263197 165263256 60 + 1.333 1.159 -0.615
ENSG00000109466 E018 25.0365468 0.0476465897 4.951074e-01 6.319633e-01 4 165263257 165263359 103 + 1.406 1.329 -0.269
ENSG00000109466 E019 31.0597791 0.0322919662 1.074549e-01 2.017823e-01 4 165294359 165294468 110 + 1.518 1.344 -0.602
ENSG00000109466 E020 35.8977855 0.0239449069 7.346016e-01 8.255216e-01 4 165297609 165297725 117 + 1.543 1.513 -0.105
ENSG00000109466 E021 34.9019233 0.0006313915 5.428018e-01 6.735100e-01 4 165299507 165299656 150 + 1.531 1.507 -0.084
ENSG00000109466 E022 28.1066050 0.0009067976 9.920725e-01 9.991396e-01 4 165305608 165305725 118 + 1.423 1.436 0.046
ENSG00000109466 E023 40.8706258 0.0005856098 1.753167e-02 4.610834e-02 4 165310553 165310750 198 + 1.622 1.492 -0.444
ENSG00000109466 E024 0.0000000       4 165310751 165310752 2 +      
ENSG00000109466 E025 32.7234821 0.0006546672 2.391254e-01 3.730829e-01 4 165311464 165311565 102 + 1.516 1.452 -0.217
ENSG00000109466 E026 37.1375324 0.0006387078 6.997687e-01 7.993987e-01 4 165313238 165313366 129 + 1.536 1.574 0.128
ENSG00000109466 E027 42.1471104 0.0012553188 1.782889e-01 2.989927e-01 4 165314026 165314166 141 + 1.572 1.663 0.310
ENSG00000109466 E028 42.8166405 0.0005906134 1.476394e-02 3.995919e-02 4 165317826 165317969 144 + 1.556 1.702 0.498
ENSG00000109466 E029 9.0840876 0.0023437384 1.153704e-02 3.243537e-02 4 165321312 165321375 64 + 0.853 1.142 1.073
ENSG00000109466 E030 98.3504497 0.0090490077 1.867652e-06 1.462735e-05 4 165322032 165323156 1125 + 1.866 2.128 0.879