ENSG00000109458

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262994 ENSG00000109458 HEK293_OSMI2_2hA HEK293_TMG_2hB GAB1 protein_coding protein_coding 3.429642 0.9909362 5.401084 0.06986999 0.7855938 2.434568 0.7528058 0.0000000 1.5189871 0.00000000 0.06367208 7.256432 0.18237500 0.0000 0.29210000 0.29210000 5.582035e-07 3.117128e-18 FALSE TRUE
ENST00000262995 ENSG00000109458 HEK293_OSMI2_2hA HEK293_TMG_2hB GAB1 protein_coding protein_coding 3.429642 0.9909362 5.401084 0.06986999 0.7855938 2.434568 1.0738958 0.5069700 1.7073621 0.05261128 0.12024931 1.732042 0.38875000 0.5125 0.32656667 -0.18593333 1.210479e-01 3.117128e-18 FALSE TRUE
ENST00000505913 ENSG00000109458 HEK293_OSMI2_2hA HEK293_TMG_2hB GAB1 protein_coding protein_coding 3.429642 0.9909362 5.401084 0.06986999 0.7855938 2.434568 0.2383325 0.4485842 0.0000000 0.04460177 0.00000000 -5.519115 0.14093750 0.4562 0.00000000 -0.45620000 3.117128e-18 3.117128e-18 FALSE TRUE
ENST00000507070 ENSG00000109458 HEK293_OSMI2_2hA HEK293_TMG_2hB GAB1 protein_coding retained_intron 3.429642 0.9909362 5.401084 0.06986999 0.7855938 2.434568 0.2166840 0.0000000 0.8625415 0.00000000 0.47028884 6.447152 0.04462083 0.0000 0.15490000 0.15490000 4.241592e-01 3.117128e-18 FALSE FALSE
ENST00000507334 ENSG00000109458 HEK293_OSMI2_2hA HEK293_TMG_2hB GAB1 protein_coding retained_intron 3.429642 0.9909362 5.401084 0.06986999 0.7855938 2.434568 0.5462686 0.0000000 0.9034658 0.00000000 0.54607715 6.513279 0.10145833 0.0000 0.14516667 0.14516667 4.227753e-01 3.117128e-18 FALSE TRUE
ENST00000510615 ENSG00000109458 HEK293_OSMI2_2hA HEK293_TMG_2hB GAB1 protein_coding retained_intron 3.429642 0.9909362 5.401084 0.06986999 0.7855938 2.434568 0.2303985 0.0000000 0.2977771 0.00000000 0.29777705 4.943814 0.04915417 0.0000 0.05843333 0.05843333 1.000000e+00 3.117128e-18 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000109458 E001 3.9190560 0.0040196008 7.488812e-01 8.359847e-01 4 143336762 143336875 114 + 0.657 0.601 -0.245
ENSG00000109458 E002 13.6612551 0.0013135308 1.128074e-02 3.183197e-02 4 143336876 143337177 302 + 1.176 0.879 -1.090
ENSG00000109458 E003 11.2461619 0.0084237074 5.004748e-02 1.092560e-01 4 143337178 143337260 83 + 1.097 0.843 -0.948
ENSG00000109458 E004 0.0000000       4 143376825 143376896 72 +      
ENSG00000109458 E005 0.1472490 0.0427839236 1.000000e+00   4 143380612 143380739 128 + 0.071 0.000 -10.732
ENSG00000109458 E006 0.0000000       4 143381945 143381947 3 +      
ENSG00000109458 E007 0.1482932 0.0409572842 1.315322e-01   4 143381948 143382030 83 + 0.000 0.203 11.926
ENSG00000109458 E008 0.0000000       4 143391444 143391509 66 +      
ENSG00000109458 E009 0.0000000       4 143391510 143391558 49 +      
ENSG00000109458 E010 0.0000000       4 143391559 143391632 74 +      
ENSG00000109458 E011 0.5869318 0.2824775744 3.332370e-01 4.769264e-01 4 143394817 143395720 904 + 0.131 0.335 1.718
ENSG00000109458 E012 0.0000000       4 143395817 143395989 173 +      
ENSG00000109458 E013 10.8483214 0.0018665821 6.070478e-02 1.278199e-01 4 143415477 143415508 32 + 1.079 0.844 -0.878
ENSG00000109458 E014 27.1022308 0.0007690972 2.224769e-02 5.618704e-02 4 143415509 143415771 263 + 1.438 1.249 -0.657
ENSG00000109458 E015 0.0000000       4 143417433 143417550 118 +      
ENSG00000109458 E016 29.2172153 0.0565988733 8.883366e-02 1.733314e-01 4 143433491 143433716 226 + 1.476 1.230 -0.858
ENSG00000109458 E017 0.2903454 0.3538316439 1.000000e+00   4 143434088 143434168 81 + 0.131 0.000 -10.989
ENSG00000109458 E018 36.5883189 0.0006854037 5.841773e-02 1.239188e-01 4 143437999 143438256 258 + 1.551 1.417 -0.460
ENSG00000109458 E019 43.4919770 0.0005693038 3.233857e-02 7.650978e-02 4 143438257 143438600 344 + 1.628 1.490 -0.472
ENSG00000109458 E020 0.3030308 0.3083157364 1.000000e+00   4 143439650 143439672 23 + 0.132 0.000 -11.046
ENSG00000109458 E021 0.4482035 0.0258468450 5.107327e-01 6.459107e-01 4 143439673 143439801 129 + 0.185 0.000 -12.386
ENSG00000109458 E022 20.7312274 0.0009312737 1.396896e-04 7.021992e-04 4 143439802 143439887 86 + 1.358 0.972 -1.381
ENSG00000109458 E023 0.1515154 0.0425563867 1.000000e+00   4 143439888 143440016 129 + 0.071 0.000 -10.734
ENSG00000109458 E024 24.2402975 0.0008123970 4.218115e-04 1.858005e-03 4 143440079 143440172 94 + 1.415 1.092 -1.137
ENSG00000109458 E025 35.4384787 0.0005655518 1.245967e-01 2.265565e-01 4 143440173 143440382 210 + 1.535 1.427 -0.374
ENSG00000109458 E026 9.9764504 0.0350954824 8.824191e-01 9.287134e-01 4 143457680 143457769 90 + 0.986 0.999 0.049
ENSG00000109458 E027 22.9975952 0.0012190516 6.392903e-01 7.527020e-01 4 143459385 143459478 94 + 1.319 1.353 0.117
ENSG00000109458 E028 35.0584387 0.0006005627 6.647986e-01 7.725684e-01 4 143460364 143460487 124 + 1.498 1.522 0.083
ENSG00000109458 E029 0.1451727 0.0430883647 1.000000e+00   4 143465997 143466102 106 + 0.071 0.000 -10.730
ENSG00000109458 E030 18.7777556 0.0012321581 8.788514e-01 9.261648e-01 4 143466103 143466116 14 + 1.251 1.234 -0.061
ENSG00000109458 E031 31.0037801 0.0007065690 5.091717e-01 6.445138e-01 4 143466117 143466225 109 + 1.468 1.417 -0.176
ENSG00000109458 E032 29.5198285 0.0008130060 4.748190e-01 6.136159e-01 4 143469031 143469163 133 + 1.418 1.464 0.160
ENSG00000109458 E033 236.0783049 0.0002642045 3.868600e-18 1.980261e-16 4 143469164 143474568 5405 + 2.271 2.441 0.566