ENSG00000109445

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262990 ENSG00000109445 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF330 protein_coding protein_coding 44.75582 37.47856 51.73873 4.004776 1.593338 0.465073 32.522825 27.187200 37.433103 3.5093834 0.4160352 0.4612419 0.72631250 0.72333333 0.72530000 0.001966667 1.0000000000 1.865641e-09 FALSE  
ENST00000514826 ENSG00000109445 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF330 protein_coding retained_intron 44.75582 37.47856 51.73873 4.004776 1.593338 0.465073 4.723687 5.786278 4.299154 1.1970548 0.5207294 -0.4277221 0.11368750 0.15633333 0.08263333 -0.073700000 0.1622351287 1.865641e-09    
ENST00000515453 ENSG00000109445 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF330 protein_coding processed_transcript 44.75582 37.47856 51.73873 4.004776 1.593338 0.465073 3.821279 1.613039 5.752934 0.2228297 0.4019049 1.8281058 0.08044167 0.04406667 0.11096667 0.066900000 0.0001894616 1.865641e-09    
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000109445 E001 0.9996463 0.0134869656 8.729512e-01 9.223432e-01 4 141220887 141220894 8 + 0.279 0.313 0.231
ENSG00000109445 E002 1.2994549 0.0158097820 8.580780e-01 9.124288e-01 4 141220895 141220899 5 + 0.343 0.382 0.230
ENSG00000109445 E003 9.2568809 0.0018170515 3.814944e-01 5.257860e-01 4 141220900 141220910 11 + 0.960 1.063 0.383
ENSG00000109445 E004 70.1624286 0.0051253487 3.602674e-01 5.045880e-01 4 141220911 141220927 17 + 1.823 1.885 0.209
ENSG00000109445 E005 70.8633105 0.0038950287 2.910437e-01 4.318638e-01 4 141220928 141220929 2 + 1.825 1.891 0.224
ENSG00000109445 E006 93.4720427 0.0003554401 1.353765e-03 5.155524e-03 4 141220930 141220933 4 + 1.908 2.038 0.437
ENSG00000109445 E007 305.9148981 0.0016361535 7.858858e-04 3.208908e-03 4 141220934 141221016 83 + 2.438 2.535 0.325
ENSG00000109445 E008 304.2381894 0.0016084939 1.634476e-03 6.071500e-03 4 141221017 141221054 38 + 2.439 2.531 0.307
ENSG00000109445 E009 269.3026948 0.0029180656 5.728962e-03 1.789770e-02 4 141221055 141221077 23 + 2.384 2.479 0.319
ENSG00000109445 E010 271.5029355 0.0030487440 7.206018e-03 2.176060e-02 4 141221078 141221108 31 + 2.388 2.482 0.313
ENSG00000109445 E011 351.7106139 0.0011576155 6.357582e-03 1.955172e-02 4 141222366 141222477 112 + 2.513 2.586 0.246
ENSG00000109445 E012 129.5762310 0.0010050478 9.640271e-01 9.813176e-01 4 141222478 141222491 14 + 2.115 2.125 0.031
ENSG00000109445 E013 32.2779560 0.0053140964 6.943496e-01 7.952028e-01 4 141222492 141222876 385 + 1.508 1.545 0.125
ENSG00000109445 E014 19.8533760 0.0009560175 2.915444e-12 6.631315e-11 4 141223769 141224019 251 + 1.520 0.942 -2.051
ENSG00000109445 E015 123.0874025 0.0004532853 6.929841e-04 2.873359e-03 4 141224487 141224506 20 + 2.144 2.046 -0.329
ENSG00000109445 E016 165.1156026 0.0004354812 2.498671e-06 1.903820e-05 4 141224607 141224677 71 + 2.281 2.158 -0.409
ENSG00000109445 E017 178.8710344 0.0019956370 1.213976e-02 3.386750e-02 4 141226767 141226846 80 + 2.293 2.224 -0.232
ENSG00000109445 E018 248.8881563 0.0011038441 3.777418e-03 1.251829e-02 4 141229571 141229697 127 + 2.433 2.367 -0.217
ENSG00000109445 E019 1.6251755 0.0086452387 1.886949e-01 3.120923e-01 4 141229698 141229698 1 + 0.532 0.313 -1.186
ENSG00000109445 E020 248.6693975 0.0006641106 1.906307e-04 9.238787e-04 4 141230166 141230270 105 + 2.440 2.359 -0.268
ENSG00000109445 E021 193.1113296 0.0002432718 3.133485e-03 1.065762e-02 4 141231439 141231485 47 + 2.324 2.261 -0.209
ENSG00000109445 E022 336.5102268 0.0007659752 1.329160e-05 8.580863e-05 4 141232525 141232642 118 + 2.574 2.490 -0.280
ENSG00000109445 E023 815.1950345 0.0016091717 7.257620e-01 8.189684e-01 4 141233715 141234697 983 + 2.905 2.923 0.060