ENSG00000109189

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000441222 ENSG00000109189 HEK293_OSMI2_2hA HEK293_TMG_2hB USP46 protein_coding protein_coding 8.137991 3.64923 13.03619 0.1197972 0.1768141 1.834016 0.78976926 0.5386406 1.291410 0.09580603 0.06927905 1.246142 0.124554167 0.1463333 0.09896667 -0.04736667 2.728650e-01 2.871763e-08 FALSE TRUE
ENST00000451218 ENSG00000109189 HEK293_OSMI2_2hA HEK293_TMG_2hB USP46 protein_coding protein_coding 8.137991 3.64923 13.03619 0.1197972 0.1768141 1.834016 4.27675636 2.4189353 6.101942 0.22315144 0.13573655 1.331307 0.568179167 0.6612667 0.46816667 -0.19310000 8.184623e-03 2.871763e-08 FALSE TRUE
ENST00000514536 ENSG00000109189 HEK293_OSMI2_2hA HEK293_TMG_2hB USP46 protein_coding nonsense_mediated_decay 8.137991 3.64923 13.03619 0.1197972 0.1768141 1.834016 0.03325055 0.1842213 0.000000 0.03404061 0.00000000 -4.279629 0.009695833 0.0500000 0.00000000 -0.05000000 2.871763e-08 2.871763e-08 FALSE TRUE
MSTRG.24873.5 ENSG00000109189 HEK293_OSMI2_2hA HEK293_TMG_2hB USP46 protein_coding   8.137991 3.64923 13.03619 0.1197972 0.1768141 1.834016 2.22145106 0.4766583 3.903489 0.24891247 0.28235221 3.007474 0.219804167 0.1334333 0.29896667 0.16553333 4.941712e-01 2.871763e-08 FALSE TRUE
MSTRG.24873.6 ENSG00000109189 HEK293_OSMI2_2hA HEK293_TMG_2hB USP46 protein_coding   8.137991 3.64923 13.03619 0.1197972 0.1768141 1.834016 0.67747107 0.0000000 1.527916 0.00000000 0.28081798 7.264833 0.057658333 0.0000000 0.11786667 0.11786667 2.075804e-07 2.871763e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000109189 E001 1.4885357 0.0511421972 8.859462e-01 0.9310417875 4 52590960 52591026 67 - 0.370 0.421 0.284
ENSG00000109189 E002 11.3512762 0.0122402161 4.073663e-04 0.0018029700 4 52591027 52591372 346 - 0.899 1.315 1.507
ENSG00000109189 E003 36.4291031 0.0028011620 2.401845e-03 0.0084667913 4 52591373 52592713 1341 - 1.460 1.684 0.763
ENSG00000109189 E004 27.8833517 0.0007247540 8.085208e-01 0.8783745420 4 52592714 52594177 1464 - 1.418 1.430 0.042
ENSG00000109189 E005 374.3551885 0.0046111483 3.129637e-05 0.0001853968 4 52594178 52596813 2636 - 2.487 2.637 0.499
ENSG00000109189 E006 73.0915352 0.0004242878 1.183145e-01 0.2175236130 4 52596814 52596989 176 - 1.804 1.902 0.331
ENSG00000109189 E007 123.6219535 0.0003245215 2.011206e-01 0.3275874515 4 52596990 52597466 477 - 2.035 2.108 0.245
ENSG00000109189 E008 55.2896876 0.0006386063 4.643771e-01 0.6041066737 4 52597467 52597580 114 - 1.691 1.758 0.227
ENSG00000109189 E009 50.5896591 0.0004970766 3.027139e-01 0.4446185890 4 52597581 52597741 161 - 1.680 1.654 -0.088
ENSG00000109189 E010 4.2920286 0.0038479023 7.821239e-01 0.8601042870 4 52598569 52598627 59 - 0.668 0.733 0.273
ENSG00000109189 E011 43.1084600 0.0059262541 1.444321e-02 0.0392287093 4 52598628 52598706 79 - 1.642 1.497 -0.497
ENSG00000109189 E012 63.7593381 0.0004436187 1.285791e-02 0.0355838624 4 52601857 52602054 198 - 1.797 1.704 -0.314
ENSG00000109189 E013 39.5779019 0.0005898847 6.259106e-01 0.7419528221 4 52604501 52604584 84 - 1.572 1.572 0.002
ENSG00000109189 E014 41.3381908 0.0005317222 7.684668e-01 0.8503871162 4 52610541 52610617 77 - 1.585 1.597 0.042
ENSG00000109189 E015 45.7471509 0.0005300230 5.700751e-02 0.1214741355 4 52626018 52626092 75 - 1.653 1.573 -0.275
ENSG00000109189 E016 38.4867189 0.0064223552 3.420625e-01 0.4860470695 4 52626093 52626127 35 - 1.570 1.526 -0.150
ENSG00000109189 E017 51.3140343 0.0017003418 3.147157e-02 0.0748304905 4 52626128 52626247 120 - 1.706 1.610 -0.326
ENSG00000109189 E018 53.7738932 0.0005033085 1.213004e-04 0.0006198888 4 52627950 52628060 111 - 1.745 1.560 -0.629
ENSG00000109189 E019 54.2358409 0.0004398508 7.023194e-05 0.0003812169 4 52628061 52628163 103 - 1.751 1.560 -0.650
ENSG00000109189 E020 0.0000000       4 52628164 52628366 203 -      
ENSG00000109189 E021 0.5954526 0.0183271545 2.408964e-01 0.3752310405 4 52629634 52629685 52 - 0.248 0.001 -9.127
ENSG00000109189 E022 33.9467365 0.0006373496 4.106654e-04 0.0018150694 4 52631064 52631108 45 - 1.561 1.341 -0.756
ENSG00000109189 E023 30.6958432 0.0006888933 2.700795e-03 0.0093766850 4 52631109 52631144 36 - 1.509 1.319 -0.658
ENSG00000109189 E024 0.0000000       4 52650971 52651088 118 -      
ENSG00000109189 E025 0.0000000       4 52655295 52655422 128 -      
ENSG00000109189 E026 0.0000000       4 52656296 52656452 157 -      
ENSG00000109189 E027 0.0000000       4 52656453 52656572 120 -      
ENSG00000109189 E028 0.0000000       4 52656573 52656590 18 -      
ENSG00000109189 E029 7.3262211 0.0024431461 3.093956e-01 0.4516835569 4 52658174 52658281 108 - 0.919 0.814 -0.407
ENSG00000109189 E030 38.8236176 0.0007932697 8.027396e-03 0.0238506649 4 52659115 52659301 187 - 1.598 1.456 -0.487