ENSG00000109133

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000264452 ENSG00000109133 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM33 protein_coding nonsense_mediated_decay 27.31838 11.24985 37.17857 0.6288846 1.037076 1.723672 1.3572308 0.1527960 2.7941879 0.07535255 0.2494904 4.1064456 0.03739167 0.01333333 0.07516667 0.06183333 0.0006313473 0.0006313473    
ENST00000325094 ENSG00000109133 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM33 protein_coding protein_coding 27.31838 11.24985 37.17857 0.6288846 1.037076 1.723672 7.2582387 4.9434935 9.5762560 0.86673652 0.1607897 0.9525211 0.31094583 0.44573333 0.25813333 -0.18760000 0.1717777205 0.0006313473 TRUE  
ENST00000504986 ENSG00000109133 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM33 protein_coding protein_coding 27.31838 11.24985 37.17857 0.6288846 1.037076 1.723672 6.3642666 2.2828539 10.1099367 0.31068962 0.5065015 2.1419839 0.22015000 0.20593333 0.27160000 0.06566667 0.4068173348 0.0006313473 FALSE  
ENST00000513558 ENSG00000109133 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM33 protein_coding protein_coding 27.31838 11.24985 37.17857 0.6288846 1.037076 1.723672 0.4877399 0.9102536 0.3082329 0.44750954 0.1562857 -1.5319484 0.02951667 0.08350000 0.00810000 -0.07540000 0.0921721521 0.0006313473 FALSE  
ENST00000513702 ENSG00000109133 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM33 protein_coding protein_coding 27.31838 11.24985 37.17857 0.6288846 1.037076 1.723672 1.4568738 0.1894394 2.4911755 0.03723970 0.3922829 3.6485841 0.04023750 0.01676667 0.06716667 0.05040000 0.0006383131 0.0006313473 FALSE  
MSTRG.24789.8 ENSG00000109133 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM33 protein_coding   27.31838 11.24985 37.17857 0.6288846 1.037076 1.723672 8.9302533 2.5812265 9.2035250 1.87914746 0.3506107 1.8301181 0.31648750 0.21766667 0.24750000 0.02983333 0.8120370079 0.0006313473 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000109133 E001 11.3122263 0.0093797229 1.985701e-01 3.244010e-01 4 41935129 41935151 23 + 1.102 0.950 -0.557
ENSG00000109133 E002 124.5717793 0.0023717231 6.635832e-01 7.715914e-01 4 41935152 41935262 111 + 2.074 2.058 -0.053
ENSG00000109133 E003 118.3417396 0.0049856757 3.549614e-01 4.993049e-01 4 41935263 41935310 48 + 2.032 2.077 0.149
ENSG00000109133 E004 132.6949805 0.0022939497 1.234112e-02 3.435137e-02 4 41935311 41935383 73 + 2.062 2.163 0.338
ENSG00000109133 E005 118.1203829 0.0002422994 1.477927e-03 5.565936e-03 4 41935384 41935428 45 + 2.009 2.123 0.384
ENSG00000109133 E006 95.8678953 0.0002993347 1.138395e-01 2.111160e-01 4 41935429 41935443 15 + 1.934 2.001 0.223
ENSG00000109133 E007 99.5882843 0.0002954494 1.302868e-02 3.597919e-02 4 41935444 41935466 23 + 1.939 2.038 0.331
ENSG00000109133 E008 154.9713606 0.0033777837 7.628818e-02 1.535645e-01 4 41935467 41935529 63 + 2.139 2.211 0.240
ENSG00000109133 E009 187.1649812 0.0005433631 4.337726e-01 5.761788e-01 4 41938602 41938696 95 + 2.237 2.264 0.092
ENSG00000109133 E010 164.1625559 0.0002438381 5.037416e-01 6.396973e-01 4 41939196 41939254 59 + 2.180 2.205 0.085
ENSG00000109133 E011 271.4362113 0.0002498317 7.452556e-01 8.333602e-01 4 41939255 41939383 129 + 2.407 2.404 -0.009
ENSG00000109133 E012 3.0217079 0.0058670036 8.960461e-03 2.618033e-02 4 41939721 41939828 108 + 0.683 0.184 -2.854
ENSG00000109133 E013 227.6822908 0.0001964894 6.176762e-01 7.354420e-01 4 41943747 41943814 68 + 2.324 2.342 0.060
ENSG00000109133 E014 1.1982920 0.1173687416 3.586839e-01 5.030552e-01 4 41943815 41944202 388 + 0.382 0.183 -1.428
ENSG00000109133 E015 302.9195731 0.0012813461 1.566114e-01 2.705866e-01 4 41944793 41944926 134 + 2.439 2.478 0.131
ENSG00000109133 E016 251.1656166 0.0002221008 4.820521e-02 1.059964e-01 4 41949302 41949385 84 + 2.354 2.407 0.176
ENSG00000109133 E017 3.3663724 0.0054124538 6.999532e-04 2.898956e-03 4 41953760 41954069 310 + 0.381 0.895 2.286
ENSG00000109133 E018 1473.0666817 0.0014304538 1.137798e-17 5.541195e-16 4 41954070 41958173 4104 + 3.175 3.041 -0.445
ENSG00000109133 E019 192.0836528 0.0002174391 4.253922e-09 5.611285e-08 4 41958174 41958871 698 + 2.301 2.134 -0.561
ENSG00000109133 E020 669.4784845 0.0176695397 6.502571e-03 1.992902e-02 4 41958872 41960803 1932 + 2.731 2.909 0.592
ENSG00000109133 E021 0.7415557 0.1095412212 7.189739e-01 8.139570e-01 4 41978134 41978282 149 + 0.256 0.183 -0.616