ENSG00000109084

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000226230 ENSG00000109084 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM97 protein_coding protein_coding 48.71912 58.14788 40.92177 2.389399 0.8674843 -0.5067536 39.735108 51.605616 31.479870 2.4683239 0.6071426 -0.71291982 0.81246667 0.88733333 0.77026667 -0.11706667 0.01529007 0.01529007 FALSE TRUE
MSTRG.13941.1 ENSG00000109084 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM97 protein_coding   48.71912 58.14788 40.92177 2.389399 0.8674843 -0.5067536 3.644426 3.653313 3.794188 0.4121826 0.3900427 0.05443927 0.07573750 0.06353333 0.09316667 0.02963333 0.34590421 0.01529007 FALSE TRUE
MSTRG.13941.2 ENSG00000109084 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM97 protein_coding   48.71912 58.14788 40.92177 2.389399 0.8674843 -0.5067536 4.714141 2.109844 5.147577 0.9300970 1.3231202 1.28273553 0.09866667 0.03626667 0.12463333 0.08836667 0.05146211 0.01529007 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000109084 E001 3.5581335 0.2035799314 0.6597263643 0.7684982786 17 28319092 28319137 46 + 0.665 0.640 -0.106
ENSG00000109084 E002 36.4208126 0.0130446706 0.0007780257 0.0031801038 17 28319138 28319199 62 + 1.682 1.460 -0.759
ENSG00000109084 E003 38.0523164 0.0097437317 0.0001940454 0.0009383874 17 28319200 28319201 2 + 1.703 1.475 -0.779
ENSG00000109084 E004 49.9303360 0.0099509808 0.0031172231 0.0106110714 17 28319202 28319205 4 + 1.782 1.623 -0.539
ENSG00000109084 E005 194.7751954 0.0085216611 0.1984681592 0.3242987524 17 28319206 28319259 54 + 2.282 2.263 -0.061
ENSG00000109084 E006 186.5753288 0.0076075148 0.3820343355 0.5262889671 17 28319260 28319268 9 + 2.249 2.253 0.014
ENSG00000109084 E007 252.5007758 0.0070071732 0.2418131042 0.3763340465 17 28319269 28319332 64 + 2.387 2.380 -0.021
ENSG00000109084 E008 212.2937513 0.0071621826 0.5408084566 0.6718991355 17 28319333 28319365 33 + 2.296 2.314 0.059
ENSG00000109084 E009 0.5138669 0.0217681645 0.3034069619 0.4453189132 17 28319455 28319697 243 + 0.256 0.102 -1.610
ENSG00000109084 E010 0.5181333 0.0217681645 0.3032757131 0.4451745611 17 28324690 28324724 35 + 0.257 0.102 -1.610
ENSG00000109084 E011 139.2196861 0.0067021539 0.8599400965 0.9136866540 17 28325503 28325507 5 + 2.101 2.139 0.127
ENSG00000109084 E012 383.6173554 0.0023102667 0.0255269356 0.0629671282 17 28325508 28325647 140 + 2.575 2.559 -0.053
ENSG00000109084 E013 1.6793230 0.1201057482 0.8830115651 0.9291278353 17 28325648 28325835 188 + 0.416 0.413 -0.017
ENSG00000109084 E014 947.1807527 0.0002146345 0.0011033847 0.0043143158 17 28326534 28327104 571 + 2.952 2.963 0.035
ENSG00000109084 E015 2002.2143821 0.0087450608 0.0095443088 0.0276448745 17 28327105 28328685 1581 + 3.200 3.320 0.399
ENSG00000109084 E016 10.6670546 0.0017722434 0.0114013530 0.0321201575 17 28329047 28329147 101 + 1.179 0.974 -0.744