ENSG00000109083

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000395418 ENSG00000109083 HEK293_OSMI2_2hA HEK293_TMG_2hB IFT20 protein_coding protein_coding 15.71567 10.52468 20.51028 1.047971 0.9710407 0.961904 8.2942473 6.4942380 9.612234 0.79251233 0.24822245 0.5649918 0.52636250 0.61460000 0.46983333 -0.14476667 0.000719743 0.000719743 FALSE TRUE
ENST00000578985 ENSG00000109083 HEK293_OSMI2_2hA HEK293_TMG_2hB IFT20 protein_coding protein_coding 15.71567 10.52468 20.51028 1.047971 0.9710407 0.961904 0.9022171 0.1224472 1.174729 0.12244724 0.59678981 3.1610676 0.05113333 0.01206667 0.05463333 0.04256667 0.585741961 0.000719743 FALSE TRUE
ENST00000583796 ENSG00000109083 HEK293_OSMI2_2hA HEK293_TMG_2hB IFT20 protein_coding retained_intron 15.71567 10.52468 20.51028 1.047971 0.9710407 0.961904 1.3927290 0.9586552 1.145708 0.01845498 0.02679248 0.2547213 0.10095000 0.09253333 0.05623333 -0.03630000 0.083618979 0.000719743   FALSE
ENST00000585089 ENSG00000109083 HEK293_OSMI2_2hA HEK293_TMG_2hB IFT20 protein_coding protein_coding 15.71567 10.52468 20.51028 1.047971 0.9710407 0.961904 3.2872670 2.0497067 5.087421 0.17125860 0.04864627 1.3073287 0.20177500 0.19536667 0.24936667 0.05400000 0.197425027 0.000719743 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000109083 E001 0.1451727 0.0436854005 7.915303e-01   17 28328325 28328325 1 - 0.099 0.000 -8.230
ENSG00000109083 E002 0.8021360 0.6269055520 7.905980e-01 8.660600e-01 17 28328326 28328326 1 - 0.303 0.156 -1.226
ENSG00000109083 E003 0.9838404 0.4506629429 8.702212e-01 9.205215e-01 17 28328327 28328328 2 - 0.304 0.269 -0.241
ENSG00000109083 E004 1.7599506 0.3271189663 4.808050e-01 6.190250e-01 17 28328329 28328336 8 - 0.356 0.504 0.786
ENSG00000109083 E005 86.3548631 0.0004198111 6.329328e-08 6.679696e-07 17 28328337 28328657 321 - 1.844 2.041 0.662
ENSG00000109083 E006 83.4409897 0.0003653639 5.509014e-02 1.181567e-01 17 28328658 28328733 76 - 1.890 1.962 0.240
ENSG00000109083 E007 16.7009451 0.0011617591 2.474569e-02 6.135351e-02 17 28328734 28328886 153 - 1.154 1.338 0.649
ENSG00000109083 E008 13.2999985 0.0105072597 1.613467e-02 4.303161e-02 17 28328887 28328998 112 - 1.029 1.267 0.854
ENSG00000109083 E009 9.4903853 0.0020247697 2.927095e-01 4.336970e-01 17 28328999 28329068 70 - 0.962 1.072 0.406
ENSG00000109083 E010 10.4422374 0.0170993504 2.863729e-01 4.267683e-01 17 28329069 28329172 104 - 0.997 1.116 0.434
ENSG00000109083 E011 105.3911889 0.0003334055 9.619914e-01 9.800248e-01 17 28329173 28329276 104 - 2.025 2.027 0.007
ENSG00000109083 E012 13.7735915 0.0013261839 3.471738e-01 4.913566e-01 17 28329277 28329684 408 - 1.130 1.214 0.300
ENSG00000109083 E013 8.4688725 0.0020908469 4.778235e-01 6.163191e-01 17 28329685 28330110 426 - 1.008 0.928 -0.295
ENSG00000109083 E014 4.5175524 0.0075965563 7.008458e-01 8.002094e-01 17 28330111 28330190 80 - 0.767 0.712 -0.223
ENSG00000109083 E015 4.2971368 0.0234388502 7.823356e-01 8.602422e-01 17 28330191 28330228 38 - 0.706 0.745 0.161
ENSG00000109083 E016 8.1572442 0.0021816013 2.417632e-01 3.762718e-01 17 28330229 28330442 214 - 0.910 1.040 0.482
ENSG00000109083 E017 72.5409066 0.0004659546 3.820152e-01 5.262740e-01 17 28330443 28330476 34 - 1.882 1.847 -0.117
ENSG00000109083 E018 84.7683937 0.0122295477 4.824520e-01 6.205804e-01 17 28330477 28330528 52 - 1.945 1.916 -0.099
ENSG00000109083 E019 2.0220075 0.1145852550 3.441362e-01 4.881918e-01 17 28331386 28331401 16 - 0.403 0.586 0.903
ENSG00000109083 E020 4.7306948 0.0207904551 1.910041e-01 3.150103e-01 17 28331402 28331602 201 - 0.837 0.635 -0.824
ENSG00000109083 E021 3.2578383 0.0050813460 3.685337e-01 5.129461e-01 17 28331603 28331858 256 - 0.683 0.543 -0.617
ENSG00000109083 E022 121.8719902 0.0002870284 8.279919e-02 1.639326e-01 17 28331859 28331987 129 - 2.109 2.057 -0.176
ENSG00000109083 E023 29.1692983 0.0010000704 1.680124e-02 4.449616e-02 17 28332146 28332181 36 - 1.536 1.378 -0.546
ENSG00000109083 E024 41.1618726 0.0005480916 2.256158e-05 1.381947e-04 17 28332182 28332382 201 - 1.707 1.466 -0.822
ENSG00000109083 E025 0.4741261 0.0219932281 8.984178e-01 9.392107e-01 17 28332690 28332723 34 - 0.179 0.151 -0.294
ENSG00000109083 E026 59.8219382 0.0004178866 1.709861e-03 6.312958e-03 17 28335340 28335489 150 - 1.836 1.692 -0.486