ENSG00000108474

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000225609 ENSG00000108474 HEK293_OSMI2_2hA HEK293_TMG_2hB PIGL protein_coding protein_coding 23.88654 20.38792 24.50555 0.1607034 0.250706 0.2652751 8.462121 9.1386057 8.5040548 0.75897240 0.62092784 -0.10370550 0.36000833 0.44870000 0.34746667 -0.1012333333 3.005984e-01 8.625529e-05 FALSE TRUE
ENST00000395844 ENSG00000108474 HEK293_OSMI2_2hA HEK293_TMG_2hB PIGL protein_coding protein_coding 23.88654 20.38792 24.50555 0.1607034 0.250706 0.2652751 1.345313 0.6834542 0.8175669 0.41491335 0.45467544 0.25507531 0.05548333 0.03370000 0.03316667 -0.0005333333 9.524342e-01 8.625529e-05 FALSE TRUE
ENST00000431149 ENSG00000108474 HEK293_OSMI2_2hA HEK293_TMG_2hB PIGL protein_coding processed_transcript 23.88654 20.38792 24.50555 0.1607034 0.250706 0.2652751 2.771464 1.2773712 4.0921623 0.26765721 0.22132107 1.67195649 0.11183333 0.06243333 0.16683333 0.1044000000 8.625529e-05 8.625529e-05   FALSE
ENST00000463810 ENSG00000108474 HEK293_OSMI2_2hA HEK293_TMG_2hB PIGL protein_coding protein_coding 23.88654 20.38792 24.50555 0.1607034 0.250706 0.2652751 3.522199 2.6170850 3.1463606 0.12782532 0.06429779 0.26479917 0.14497500 0.12830000 0.12843333 0.0001333333 1.000000e+00 8.625529e-05   FALSE
ENST00000488375 ENSG00000108474 HEK293_OSMI2_2hA HEK293_TMG_2hB PIGL protein_coding retained_intron 23.88654 20.38792 24.50555 0.1607034 0.250706 0.2652751 1.443988 1.0042524 1.0232354 0.07395257 0.17207254 0.02675225 0.06130417 0.04926667 0.04190000 -0.0073666667 7.867946e-01 8.625529e-05 FALSE TRUE
MSTRG.13739.1 ENSG00000108474 HEK293_OSMI2_2hA HEK293_TMG_2hB PIGL protein_coding   23.88654 20.38792 24.50555 0.1607034 0.250706 0.2652751 1.772668 1.9266320 2.1303437 0.63860863 0.44207897 0.14429263 0.07482917 0.09496667 0.08726667 -0.0077000000 9.930296e-01 8.625529e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000108474 E001 2.6508567 0.0058834851 9.310529e-01 9.605780e-01 17 16217191 16217208 18 + 0.563 0.551 -0.057
ENSG00000108474 E002 2.2811183 0.0105042676 5.475713e-01 6.776064e-01 17 16217209 16217209 1 + 0.563 0.465 -0.472
ENSG00000108474 E003 2.6508567 0.0058834851 9.310529e-01 9.605780e-01 17 16217210 16217215 6 + 0.563 0.551 -0.057
ENSG00000108474 E004 3.4238464 0.0487034874 9.657335e-01 9.824640e-01 17 16217216 16217219 4 + 0.636 0.626 -0.040
ENSG00000108474 E005 17.0322921 0.0011730460 2.332308e-01 3.662600e-01 17 16217220 16217228 9 + 1.299 1.204 -0.335
ENSG00000108474 E006 33.2636403 0.0012073429 6.035723e-01 7.243441e-01 17 16217229 16217236 8 + 1.547 1.517 -0.100
ENSG00000108474 E007 52.0467890 0.0045348891 8.704601e-01 9.206817e-01 17 16217237 16217245 9 + 1.714 1.723 0.029
ENSG00000108474 E008 147.0640893 0.0004003798 3.953769e-01 5.391898e-01 17 16217246 16217461 216 + 2.154 2.180 0.087
ENSG00000108474 E009 54.1642434 0.0004777410 3.930161e-01 5.369074e-01 17 16217548 16218219 672 + 1.759 1.722 -0.127
ENSG00000108474 E010 93.8472244 0.0003078380 2.990495e-02 7.177212e-02 17 16233971 16234070 100 + 1.931 2.010 0.266
ENSG00000108474 E011 0.9598924 0.0142582381 9.537924e-01 9.750488e-01 17 16234071 16234140 70 + 0.300 0.291 -0.059
ENSG00000108474 E012 0.4355181 0.6370179950 2.746251e-01 4.138019e-01 17 16279657 16279812 156 + 0.299 0.000 -11.827
ENSG00000108474 E013 0.6569633 0.4788998710 5.495434e-01 6.792590e-01 17 16283996 16284020 25 + 0.299 0.123 -1.590
ENSG00000108474 E014 3.9656912 0.0631760048 2.990011e-02 7.176537e-02 17 16284021 16284138 118 + 0.863 0.467 -1.703
ENSG00000108474 E015 73.9519657 0.0004086074 1.129822e-03 4.403872e-03 17 16299888 16299978 91 + 1.798 1.932 0.452
ENSG00000108474 E016 0.5169874 0.0203993686 5.934298e-01 7.158270e-01 17 16299979 16300187 209 + 0.125 0.214 0.943
ENSG00000108474 E017 1.1770712 0.0195750076 7.541087e-01 8.398260e-01 17 16312387 16312824 438 + 0.300 0.357 0.358
ENSG00000108474 E018 55.0167640 0.0117658311 1.443271e-01 2.539009e-01 17 16313547 16313614 68 + 1.691 1.791 0.339
ENSG00000108474 E019 7.4458404 0.0022913709 2.436285e-01 3.784328e-01 17 16313912 16314072 161 + 0.998 0.868 -0.491
ENSG00000108474 E020 34.9433127 0.0010533249 5.505643e-01 6.801792e-01 17 16316681 16316701 21 + 1.538 1.576 0.128
ENSG00000108474 E021 26.9260720 0.0077211007 5.886968e-01 7.118811e-01 17 16316702 16316712 11 + 1.424 1.467 0.147
ENSG00000108474 E022 6.5518254 0.0062736181 2.995492e-01 4.410749e-01 17 16316713 16316794 82 + 0.936 0.808 -0.492
ENSG00000108474 E023 24.9279796 0.0308711036 7.057509e-01 8.040750e-01 17 16316795 16317390 596 + 1.430 1.392 -0.134
ENSG00000108474 E024 15.6973835 0.0174498178 9.578765e-01 9.775938e-01 17 16317391 16317688 298 + 1.220 1.214 -0.024
ENSG00000108474 E025 7.8071557 0.0022720261 5.691993e-01 6.957936e-01 17 16317689 16317774 86 + 0.902 0.968 0.251
ENSG00000108474 E026 82.3203088 0.0008980268 8.765167e-02 1.715654e-01 17 16317775 16317908 134 + 1.885 1.955 0.235
ENSG00000108474 E027 0.3634088 0.4124014481 3.076288e-01   17 16324353 16324391 39 + 0.000 0.216 10.536
ENSG00000108474 E028 135.8829430 0.0062365242 2.030158e-02 5.211411e-02 17 16325800 16326036 237 + 2.074 2.184 0.368
ENSG00000108474 E029 92.1401656 0.0005023692 5.396017e-01 6.708486e-01 17 16326037 16326411 375 + 1.982 1.963 -0.064
ENSG00000108474 E030 40.9714195 0.0067896294 2.147684e-10 3.577528e-09 17 16326485 16326843 359 + 1.805 1.375 -1.467
ENSG00000108474 E031 17.9678841 0.0097814636 2.029275e-04 9.763804e-04 17 16326844 16326905 62 + 1.430 1.086 -1.212
ENSG00000108474 E032 7.4060159 0.1107480880 1.060039e-02 3.020973e-02 17 16342573 16342720 148 + 1.113 0.635 -1.852
ENSG00000108474 E033 3.3236154 0.0046388489 1.046737e-01 1.976273e-01 17 16343016 16343567 552 + 0.752 0.510 -1.058
ENSG00000108474 E034 0.9307487 0.0750659509 1.023590e-01 1.941200e-01 17 16343675 16343735 61 + 0.425 0.120 -2.380
ENSG00000108474 E035 1.9499748 0.0075517203 5.187446e-01 6.529571e-01 17 16344318 16344495 178 + 0.522 0.414 -0.543
ENSG00000108474 E036 7.6332387 0.0022297937 4.817354e-05 2.725336e-04 17 16347545 16347703 159 + 1.134 0.654 -1.846
ENSG00000108474 E037 6.6085090 0.0024515230 9.021287e-03 2.633124e-02 17 16348692 16348801 110 + 1.026 0.712 -1.215
ENSG00000108474 E038 16.9069002 0.0143877611 4.470967e-01 5.885252e-01 17 16350625 16351797 1173 + 1.285 1.207 -0.272