ENSG00000107485

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000346208 ENSG00000107485 HEK293_OSMI2_2hA HEK293_TMG_2hB GATA3 protein_coding protein_coding 2.24018 2.606161 1.854645 0.2439028 0.07125554 -0.4885501 0.3169091 0.6247837 0.1969345 0.09131913 0.07878649 -1.617091 0.1353958 0.2397000 0.1048 -0.1349000 0.13368150 0.04518583 FALSE TRUE
MSTRG.3515.1 ENSG00000107485 HEK293_OSMI2_2hA HEK293_TMG_2hB GATA3 protein_coding   2.24018 2.606161 1.854645 0.2439028 0.07125554 -0.4885501 1.1990967 1.3867184 0.6350777 0.15467015 0.13888564 -1.114496 0.5133708 0.5320333 0.3414 -0.1906333 0.18789341 0.04518583 FALSE TRUE
MSTRG.3515.4 ENSG00000107485 HEK293_OSMI2_2hA HEK293_TMG_2hB GATA3 protein_coding   2.24018 2.606161 1.854645 0.2439028 0.07125554 -0.4885501 0.5589683 0.3897087 0.9649631 0.08800994 0.24412761 1.286398 0.2794500 0.1565333 0.5243 0.3677667 0.04518583 0.04518583 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000107485 E001 3.6232179 0.0159881012 0.4078265209 0.551418922 10 8045256 8045377 122 + 0.567 0.709 0.615
ENSG00000107485 E002 6.9640301 0.0217520318 0.1391463198 0.246890198 10 8045378 8045515 138 + 0.749 0.965 0.834
ENSG00000107485 E003 0.2924217 0.0290785164 0.1203473981   10 8050157 8050184 28 + 0.248 0.000 -12.136
ENSG00000107485 E004 3.2775318 0.0049572224 0.0192842055 0.049953304 10 8053604 8053778 175 + 0.805 0.467 -1.480
ENSG00000107485 E005 0.1482932 0.0417962913 0.6749219621   10 8054688 8054809 122 + 0.000 0.105 9.583
ENSG00000107485 E006 0.4782907 0.0232523680 0.1560075600 0.269801762 10 8054810 8054891 82 + 0.000 0.261 11.155
ENSG00000107485 E007 32.4433585 0.0007449058 0.2943054133 0.435414621 10 8055287 8055896 610 + 1.537 1.488 -0.167
ENSG00000107485 E008 15.8975173 0.0303297947 0.4773351253 0.615892904 10 8058305 8058398 94 + 1.243 1.179 -0.227
ENSG00000107485 E009 45.2878533 0.0016723830 0.3106675173 0.453093468 10 8058399 8058841 443 + 1.666 1.628 -0.129
ENSG00000107485 E010 5.8061040 0.0036865161 0.1206303736 0.220860497 10 8063993 8063995 3 + 0.919 0.734 -0.726
ENSG00000107485 E011 27.8187999 0.0013474467 0.1120620243 0.208570980 10 8063996 8064138 143 + 1.496 1.407 -0.306
ENSG00000107485 E012 28.6366266 0.0009659835 0.1369984415 0.243909704 10 8069473 8069598 126 + 1.502 1.421 -0.277
ENSG00000107485 E013 97.6386879 0.0046974484 0.0006059845 0.002555186 10 8073739 8075198 1460 + 1.883 2.027 0.485