ENSG00000106459

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000223190 ENSG00000106459 HEK293_OSMI2_2hA HEK293_TMG_2hB NRF1 protein_coding protein_coding 14.6399 16.25176 16.35302 2.22472 0.343212 0.008955504 4.840790 4.307660 6.374488 0.7519268 0.1158893 0.5643213 0.3331292 0.26290000 0.3900333 0.12713333 1.895126e-03 1.907549e-05 FALSE TRUE
ENST00000393232 ENSG00000106459 HEK293_OSMI2_2hA HEK293_TMG_2hB NRF1 protein_coding protein_coding 14.6399 16.25176 16.35302 2.22472 0.343212 0.008955504 7.151598 9.732017 6.591294 1.0060353 0.1754404 -0.5614715 0.4837875 0.60576667 0.4031667 -0.20260000 1.907549e-05 1.907549e-05 FALSE TRUE
ENST00000477428 ENSG00000106459 HEK293_OSMI2_2hA HEK293_TMG_2hB NRF1 protein_coding processed_transcript 14.6399 16.25176 16.35302 2.22472 0.343212 0.008955504 1.028602 0.571864 1.573616 0.2372080 0.2734264 1.4444683 0.0711375 0.03226667 0.0957000 0.06343333 5.467591e-02 1.907549e-05   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000106459 E001 1.5961561 0.3452438520 5.902228e-01 7.131219e-01 7 129611212 129611719 508 + 0.498 0.340 -0.855
ENSG00000106459 E002 4.9748494 0.0531362964 6.284455e-01 7.439814e-01 7 129611720 129611738 19 + 0.802 0.741 -0.244
ENSG00000106459 E003 6.4819313 0.0451368771 8.651454e-01 9.171187e-01 7 129611739 129611746 8 + 0.869 0.863 -0.023
ENSG00000106459 E004 16.1697172 0.0028039355 4.030914e-02 9.159923e-02 7 129611747 129611755 9 + 1.312 1.159 -0.541
ENSG00000106459 E005 54.5675098 0.0048776475 3.873167e-02 8.868696e-02 7 129611756 129611824 69 + 1.795 1.695 -0.339
ENSG00000106459 E006 0.7405235 0.0151067856 4.815494e-01 6.197771e-01 7 129630068 129630077 10 + 0.315 0.203 -0.839
ENSG00000106459 E007 10.7296183 0.0019928353 1.520037e-04 7.558570e-04 7 129630078 129630168 91 + 1.248 0.895 -1.284
ENSG00000106459 E008 8.7420516 0.0113731145 1.289714e-03 4.941698e-03 7 129633445 129633780 336 + 1.161 0.803 -1.332
ENSG00000106459 E009 3.8168663 0.1833651127 8.913431e-01 9.345854e-01 7 129641725 129641822 98 + 0.660 0.672 0.051
ENSG00000106459 E010 30.6711762 0.0006787237 8.813507e-02 1.722824e-01 7 129657343 129657345 3 + 1.545 1.463 -0.282
ENSG00000106459 E011 130.2091811 0.0004324545 2.212140e-08 2.558506e-07 7 129657346 129657574 229 + 2.197 2.046 -0.503
ENSG00000106459 E012 97.8667226 0.0003444571 1.168607e-04 5.994668e-04 7 129671429 129671543 115 + 2.055 1.943 -0.376
ENSG00000106459 E013 112.2959796 0.0002979713 5.907409e-03 1.836674e-02 7 129677632 129677758 127 + 2.090 2.024 -0.224
ENSG00000106459 E014 125.0306536 0.0002640317 2.531878e-02 6.252612e-02 7 129690406 129690546 141 + 2.126 2.081 -0.152
ENSG00000106459 E015 143.3204542 0.0003011494 2.441125e-03 8.585130e-03 7 129709075 129709233 159 + 2.195 2.130 -0.217
ENSG00000106459 E016 177.5886818 0.0002220174 9.016632e-01 9.413449e-01 7 129710374 129710571 198 + 2.239 2.259 0.069
ENSG00000106459 E017 130.5050933 0.0007945586 6.463968e-01 7.583183e-01 7 129711475 129711576 102 + 2.093 2.130 0.123
ENSG00000106459 E018 156.4151820 0.0002400893 3.835901e-01 5.277989e-01 7 129717219 129717376 158 + 2.166 2.214 0.161
ENSG00000106459 E019 124.8085119 0.0002473857 2.947171e-01 4.358282e-01 7 129727241 129727365 125 + 2.066 2.123 0.191
ENSG00000106459 E020 1.4801151 0.0100763330 5.055829e-01 6.413459e-01 7 129744173 129744229 57 + 0.443 0.341 -0.573
ENSG00000106459 E021 545.2552465 0.0022560012 1.022670e-03 4.036960e-03 7 129755018 129756265 1248 + 2.677 2.770 0.310
ENSG00000106459 E022 248.5757113 0.0034787610 7.665081e-07 6.530227e-06 7 129756266 129757082 817 + 2.272 2.465 0.644