ENSG00000105866

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000222584 ENSG00000105866 HEK293_OSMI2_2hA HEK293_TMG_2hB SP4 protein_coding protein_coding 2.721707 0.7447451 5.05873 0.07148566 0.1523784 2.747563 0.7413819 0.0866088 1.96085892 0.08660880 1.19755512 4.350526 0.2202583 0.1351667 0.3878667 0.2527000 4.684451e-01 5.212704e-13 FALSE TRUE
ENST00000432066 ENSG00000105866 HEK293_OSMI2_2hA HEK293_TMG_2hB SP4 protein_coding protein_coding 2.721707 0.7447451 5.05873 0.07148566 0.1523784 2.747563 0.2241201 0.0000000 0.66304220 0.00000000 0.05290027 6.072625 0.0465125 0.0000000 0.1318667 0.1318667 1.492484e-03 5.212704e-13   FALSE
ENST00000448246 ENSG00000105866 HEK293_OSMI2_2hA HEK293_TMG_2hB SP4 protein_coding nonsense_mediated_decay 2.721707 0.7447451 5.05873 0.07148566 0.1523784 2.747563 0.2014883 0.4111357 0.03604611 0.09295943 0.01875716 -3.193134 0.2402792 0.5445667 0.0073000 -0.5372667 5.212704e-13 5.212704e-13 FALSE TRUE
ENST00000649633 ENSG00000105866 HEK293_OSMI2_2hA HEK293_TMG_2hB SP4 protein_coding protein_coding 2.721707 0.7447451 5.05873 0.07148566 0.1523784 2.747563 1.4745639 0.2470007 2.39878274 0.13066420 1.20493769 3.228460 0.4645333 0.3202667 0.4729667 0.1527000 8.993366e-01 5.212704e-13 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000105866 E001 0.8921407 0.0131182198 4.345162e-01 5.768753e-01 7 21428043 21428043 1 + 0.251 0.000 -10.578
ENSG00000105866 E002 1.7820913 0.0076890321 1.258623e-01 2.283756e-01 7 21428044 21428082 39 + 0.409 0.000 -12.627
ENSG00000105866 E003 2.5205265 0.0067566823 5.146075e-02 1.117489e-01 7 21428083 21428194 112 + 0.507 0.000 -13.031
ENSG00000105866 E004 1.6369186 0.0104439408 1.524175e-01 2.649645e-01 7 21428195 21428200 6 + 0.386 0.000 -12.496
ENSG00000105866 E005 3.7650289 0.0878294865 1.703910e-01 2.886736e-01 7 21428201 21428258 58 + 0.617 0.259 -1.948
ENSG00000105866 E006 2.2753593 0.1232591590 4.965799e-01 6.332070e-01 7 21428677 21428681 5 + 0.452 0.259 -1.170
ENSG00000105866 E007 8.9111218 0.0018418906 8.837560e-04 3.557313e-03 7 21428682 21428792 111 + 0.939 0.258 -3.242
ENSG00000105866 E008 0.5806908 0.7760989165 1.000000e+00 1.000000e+00 7 21428793 21428900 108 + 0.181 0.000 -10.208
ENSG00000105866 E009 109.5925908 0.0003788331 1.627294e-14 5.204018e-13 7 21429289 21430843 1555 + 1.958 1.546 -1.395
ENSG00000105866 E010 27.8016237 0.0070596539 1.493047e-02 4.033041e-02 7 21477079 21477307 229 + 1.367 1.093 -0.967
ENSG00000105866 E011 28.5104266 0.0056897769 8.472555e-04 3.428017e-03 7 21481924 21482123 200 + 1.386 0.998 -1.381
ENSG00000105866 E012 223.4934792 0.0004003740 1.322194e-30 2.321461e-28 7 21511022 21514822 3801 + 2.181 2.386 0.683