ENSG00000105229

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262971 ENSG00000105229 HEK293_OSMI2_2hA HEK293_TMG_2hB PIAS4 protein_coding protein_coding 27.10304 39.76858 20.51071 1.939356 0.6478518 -0.9549112 18.229008 30.150081 10.675515 2.000430 0.4623741 -1.4969838 0.64268750 0.75680000 0.5218667 -0.2349333 2.614734e-06 2.614734e-06 FALSE TRUE
ENST00000600566 ENSG00000105229 HEK293_OSMI2_2hA HEK293_TMG_2hB PIAS4 protein_coding retained_intron 27.10304 39.76858 20.51071 1.939356 0.6478518 -0.9549112 5.478516 6.632355 4.578012 1.164084 1.2120034 -0.5338258 0.21002083 0.16813333 0.2265333 0.0584000 8.028007e-01 2.614734e-06 FALSE FALSE
MSTRG.16190.2 ENSG00000105229 HEK293_OSMI2_2hA HEK293_TMG_2hB PIAS4 protein_coding   27.10304 39.76858 20.51071 1.939356 0.6478518 -0.9549112 1.746906 1.773882 4.167355 1.085545 1.3037365 1.2275698 0.08328333 0.04436667 0.1996333 0.1552667 3.552650e-01 2.614734e-06 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000105229 E001 6.7667735 0.0613723840 6.125904e-01 7.314953e-01 19 4007733 4007734 2 + 0.899 0.813 -0.335
ENSG00000105229 E002 7.1397341 0.0372337864 5.022667e-01 6.383231e-01 19 4007735 4007735 1 + 0.928 0.828 -0.384
ENSG00000105229 E003 46.3188263 0.0004847989 8.271824e-04 3.356693e-03 19 4007736 4007753 18 + 1.735 1.553 -0.619
ENSG00000105229 E004 47.1971461 0.0004718136 1.068532e-03 4.196053e-03 19 4007754 4007755 2 + 1.740 1.563 -0.601
ENSG00000105229 E005 54.0755448 0.0004511359 5.407441e-05 3.021007e-04 19 4007756 4007757 2 + 1.817 1.614 -0.688
ENSG00000105229 E006 113.4117488 0.0002563833 2.628102e-06 1.992820e-05 19 4007758 4007787 30 + 2.104 1.937 -0.557
ENSG00000105229 E007 466.9630515 0.0001911489 1.374487e-05 8.841248e-05 19 4012923 4013349 427 + 2.652 2.577 -0.251
ENSG00000105229 E008 0.0000000       19 4017703 4017800 98 +      
ENSG00000105229 E009 0.0000000       19 4018435 4018736 302 +      
ENSG00000105229 E010 226.2877503 0.0002293029 2.560492e-01 3.930021e-01 19 4024036 4024120 85 + 2.305 2.277 -0.094
ENSG00000105229 E011 0.6265839 0.0832900385 2.623313e-01 4.001117e-01 19 4024121 4024122 2 + 0.000 0.251 9.788
ENSG00000105229 E012 158.8665024 0.0002173187 2.494327e-01 3.852495e-01 19 4028146 4028187 42 + 2.098 2.137 0.132
ENSG00000105229 E013 1.5939504 0.0156009349 7.445534e-01 8.328533e-01 19 4028188 4028197 10 + 0.315 0.378 0.381
ENSG00000105229 E014 0.7061804 0.0806228496 3.578871e-01 5.023088e-01 19 4028198 4028225 28 + 0.316 0.146 -1.419
ENSG00000105229 E015 0.8836053 0.0157590018 5.356378e-01 6.674404e-01 19 4028461 4028509 49 + 0.315 0.203 -0.840
ENSG00000105229 E016 248.4182271 0.0020121716 3.409893e-01 4.849186e-01 19 4028510 4028600 91 + 2.295 2.330 0.117
ENSG00000105229 E017 297.5016148 0.0001779023 6.302894e-01 7.454749e-01 19 4028720 4028848 129 + 2.388 2.401 0.044
ENSG00000105229 E018 236.7050497 0.0002112999 8.611234e-01 9.144208e-01 19 4028931 4029036 106 + 2.294 2.301 0.022
ENSG00000105229 E019 170.3809345 0.0050020619 4.095288e-01 5.531364e-01 19 4033100 4033173 74 + 2.127 2.169 0.140
ENSG00000105229 E020 212.2793295 0.0010030758 5.997871e-01 7.211308e-01 19 4033420 4033580 161 + 2.262 2.250 -0.043
ENSG00000105229 E021 204.0611239 0.0054875428 1.929872e-01 3.175243e-01 19 4037374 4037504 131 + 2.275 2.225 -0.165
ENSG00000105229 E022 971.6531582 0.0002487431 1.059038e-14 3.470535e-13 19 4037616 4039386 1771 + 2.833 2.936 0.344