ENSG00000104549

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000265896 ENSG00000104549 HEK293_OSMI2_2hA HEK293_TMG_2hB SQLE protein_coding protein_coding 82.61344 61.61326 84.20875 6.890811 2.808039 0.4506665 55.61834 31.06094 64.61584 2.430293 0.9689017 1.0565453 0.6467750 0.5091667 0.7698667 0.2607000 0.001729281 0.001193612 FALSE TRUE
ENST00000523430 ENSG00000104549 HEK293_OSMI2_2hA HEK293_TMG_2hB SQLE protein_coding protein_coding 82.61344 61.61326 84.20875 6.890811 2.808039 0.4506665 24.73686 29.50332 16.99058 4.957866 3.6785911 -0.7957826 0.3280625 0.4737000 0.1993333 -0.2743667 0.001193612 0.001193612 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000104549 E001 4.606485 1.158499e-01 3.928368e-01 5.367262e-01 8 124998497 124998504 8 + 0.832 0.634 -0.808
ENSG00000104549 E002 77.581777 2.418205e-02 8.612135e-05 4.568485e-04 8 124998505 124998543 39 + 2.034 1.676 -1.204
ENSG00000104549 E003 138.394849 2.012626e-02 5.774023e-04 2.449990e-03 8 124998544 124998605 62 + 2.257 1.985 -0.910
ENSG00000104549 E004 141.392755 2.318780e-02 8.310714e-03 2.456991e-02 8 124998606 124998646 41 + 2.250 2.022 -0.762
ENSG00000104549 E005 1333.799777 1.906582e-03 2.556967e-10 4.202027e-09 8 124998647 124999694 1048 + 3.188 3.050 -0.459
ENSG00000104549 E006 4.127817 4.575764e-03 5.705605e-03 1.783179e-02 8 124999900 125000071 172 + 0.866 0.453 -1.789
ENSG00000104549 E007 716.593546 9.677863e-05 2.587009e-21 1.921965e-19 8 125003176 125003428 253 + 2.917 2.791 -0.418
ENSG00000104549 E008 736.554686 1.393817e-04 8.190875e-06 5.550043e-05 8 125005525 125005705 181 + 2.899 2.840 -0.196
ENSG00000104549 E009 1.180296 1.073703e-02 4.738511e-03 1.520129e-02 8 125005706 125005707 2 + 0.519 0.000 -11.219
ENSG00000104549 E010 568.236004 1.454257e-04 4.999065e-01 6.361686e-01 8 125007391 125007487 97 + 2.764 2.755 -0.029
ENSG00000104549 E011 508.077683 1.877646e-04 1.399161e-02 3.820763e-02 8 125008971 125009084 114 + 2.691 2.732 0.139
ENSG00000104549 E012 632.548629 1.260569e-04 6.476297e-03 1.986199e-02 8 125009172 125009279 108 + 2.787 2.828 0.137
ENSG00000104549 E013 543.648743 1.713440e-04 2.024039e-02 5.198663e-02 8 125009280 125009343 64 + 2.722 2.760 0.128
ENSG00000104549 E014 3.862766 3.578845e-02 4.475522e-01 5.889292e-01 8 125010945 125011536 592 + 0.750 0.631 -0.496
ENSG00000104549 E015 594.408768 1.147025e-04 1.748435e-01 2.944893e-01 8 125011537 125011632 96 + 2.768 2.790 0.073
ENSG00000104549 E016 1.437050 1.297745e-02 4.416825e-02 9.869945e-02 8 125017942 125018058 117 + 0.198 0.552 2.152
ENSG00000104549 E017 617.198378 1.060681e-03 8.635499e-04 3.486052e-03 8 125018059 125018201 143 + 2.761 2.827 0.220
ENSG00000104549 E018 510.602000 1.412246e-03 1.927801e-04 9.329350e-04 8 125018631 125018727 97 + 2.668 2.753 0.283
ENSG00000104549 E019 6.301330 2.161814e-02 5.697795e-03 1.781062e-02 8 125018728 125019113 386 + 1.022 0.632 -1.535
ENSG00000104549 E020 599.576328 1.319146e-03 2.747387e-06 2.074634e-05 8 125020784 125020871 88 + 2.729 2.831 0.340
ENSG00000104549 E021 5.195300 1.248591e-01 8.885743e-01 9.327637e-01 8 125020872 125020937 66 + 0.791 0.772 -0.074
ENSG00000104549 E022 1172.961565 2.222365e-03 3.581494e-12 8.020132e-11 8 125021753 125022283 531 + 2.988 3.145 0.522