ENSG00000104472

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000220913 ENSG00000104472 HEK293_OSMI2_2hA HEK293_TMG_2hB CHRAC1 protein_coding protein_coding 20.92262 26.17572 23.60993 1.145733 0.4510904 -0.1487754 16.706715 15.987588 20.652999 1.2507877 0.48074492 0.3691953 0.80995833 0.61320000 0.87466667 0.2614667 2.474213e-05 3.535611e-06 FALSE TRUE
ENST00000519533 ENSG00000104472 HEK293_OSMI2_2hA HEK293_TMG_2hB CHRAC1 protein_coding protein_coding 20.92262 26.17572 23.60993 1.145733 0.4510904 -0.1487754 1.159194 1.191688 1.755354 0.6121803 0.10427160 0.5548957 0.05390417 0.04706667 0.07456667 0.0275000 4.964085e-01 3.535611e-06   FALSE
ENST00000519618 ENSG00000104472 HEK293_OSMI2_2hA HEK293_TMG_2hB CHRAC1 protein_coding protein_coding 20.92262 26.17572 23.60993 1.145733 0.4510904 -0.1487754 2.453575 8.645721 0.855663 2.2825231 0.06766726 -3.3217767 0.10043333 0.32633333 0.03623333 -0.2901000 3.535611e-06 3.535611e-06   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000104472 E001 0.0000000       8 140505719 140506500 782 +      
ENSG00000104472 E002 5.3371496 0.0286531245 3.919644e-01 5.359080e-01 8 140506803 140511310 4508 + 0.812 0.771 -0.165
ENSG00000104472 E003 9.2624648 0.0169720885 2.159941e-04 1.032563e-03 8 140511311 140511321 11 + 1.159 0.794 -1.365
ENSG00000104472 E004 212.8756330 0.0067038633 2.555943e-11 4.982669e-10 8 140511322 140511646 325 + 2.416 2.233 -0.611
ENSG00000104472 E005 1.1729171 0.1967957259 1.685713e-01 2.863026e-01 8 140511918 140511981 64 + 0.116 0.447 2.561
ENSG00000104472 E006 6.5570186 0.0026404351 4.467359e-01 5.882102e-01 8 140511982 140512025 44 + 0.770 0.953 0.709
ENSG00000104472 E007 0.9619687 0.0130688945 8.878227e-01 9.322951e-01 8 140514294 140514368 75 + 0.282 0.310 0.184
ENSG00000104472 E008 247.9597768 0.0002894604 3.058075e-24 3.118101e-22 8 140514369 140514495 127 + 2.453 2.335 -0.396
ENSG00000104472 E009 7.0624701 0.0112513761 3.055293e-01 4.476432e-01 8 140514496 140515125 630 + 0.920 0.881 -0.148
ENSG00000104472 E010 1384.5704706 0.0035210291 1.741143e-21 1.316783e-19 8 140515126 140517154 2029 + 3.032 3.224 0.640