ENSG00000104450

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000251809 ENSG00000104450 HEK293_OSMI2_2hA HEK293_TMG_2hB SPAG1 protein_coding protein_coding 2.78407 1.212502 4.167181 0.196039 0.5104808 1.772693 0.3621154 0.05883468 0.5889475 0.05883468 0.3222891 3.1212219 0.0914500 0.0406000 0.1267333 0.08613333 0.7154151772 0.0002832795 FALSE TRUE
ENST00000388798 ENSG00000104450 HEK293_OSMI2_2hA HEK293_TMG_2hB SPAG1 protein_coding protein_coding 2.78407 1.212502 4.167181 0.196039 0.5104808 1.772693 0.3834948 0.00000000 0.8028851 0.00000000 0.8028851 6.3449796 0.1527792 0.0000000 0.2388333 0.23883333 1.0000000000 0.0002832795 FALSE TRUE
ENST00000519409 ENSG00000104450 HEK293_OSMI2_2hA HEK293_TMG_2hB SPAG1 protein_coding processed_transcript 2.78407 1.212502 4.167181 0.196039 0.5104808 1.772693 0.2940516 0.13608088 0.0000000 0.06855118 0.0000000 -3.8686954 0.1426792 0.0970000 0.0000000 -0.09700000 0.0047199772 0.0002832795   FALSE
ENST00000519424 ENSG00000104450 HEK293_OSMI2_2hA HEK293_TMG_2hB SPAG1 protein_coding retained_intron 2.78407 1.212502 4.167181 0.196039 0.5104808 1.772693 0.1757565 0.14978823 0.1944737 0.07936752 0.1056016 0.3557538 0.0732500 0.1072000 0.0455000 -0.06170000 0.7137967549 0.0002832795   FALSE
MSTRG.31896.2 ENSG00000104450 HEK293_OSMI2_2hA HEK293_TMG_2hB SPAG1 protein_coding   2.78407 1.212502 4.167181 0.196039 0.5104808 1.772693 0.7875650 0.83808704 1.2251505 0.03304343 0.6133225 0.5424026 0.3133542 0.7291667 0.2728333 -0.45633333 0.3617623152 0.0002832795 FALSE TRUE
MSTRG.31896.7 ENSG00000104450 HEK293_OSMI2_2hA HEK293_TMG_2hB SPAG1 protein_coding   2.78407 1.212502 4.167181 0.196039 0.5104808 1.772693 0.5987531 0.00000000 1.0440185 0.00000000 0.3335792 6.7197564 0.1638125 0.0000000 0.2406333 0.24063333 0.0002832795 0.0002832795 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000104450 E001 0.4375944 0.0237971399 4.599550e-01 6.000177e-01 8 100157906 100158034 129 + 0.190 0.000 -9.082
ENSG00000104450 E002 1.7026957 0.0084067255 2.020078e-01 3.287078e-01 8 100158035 100158118 84 + 0.452 0.198 -1.662
ENSG00000104450 E003 0.0000000       8 100158335 100158586 252 +      
ENSG00000104450 E004 0.0000000       8 100158587 100158616 30 +      
ENSG00000104450 E005 2.5820372 0.0057788975 4.503797e-02 1.002716e-01 8 100162279 100162420 142 + 0.594 0.198 -2.341
ENSG00000104450 E006 7.3409108 0.0022866388 7.677583e-04 3.142351e-03 8 100165814 100165973 160 + 0.974 0.437 -2.280
ENSG00000104450 E007 8.2261571 0.0023024184 2.547102e-05 1.541467e-04 8 100177816 100177941 126 + 1.030 0.334 -3.069
ENSG00000104450 E008 7.5426849 0.0022303085 1.836070e-01 3.057326e-01 8 100183375 100183436 62 + 0.930 0.750 -0.699
ENSG00000104450 E009 9.4756062 0.0018670502 9.312128e-01 9.606802e-01 8 100183956 100184062 107 + 0.974 0.986 0.042
ENSG00000104450 E010 9.3607362 0.0018751047 6.450892e-01 7.572777e-01 8 100184628 100184733 106 + 0.957 1.011 0.199
ENSG00000104450 E011 9.3962992 0.0018597667 8.889271e-01 9.329583e-01 8 100187120 100187250 131 + 0.974 0.959 -0.057
ENSG00000104450 E012 8.6461011 0.0203048890 1.658051e-01 2.827501e-01 8 100191390 100191496 107 + 0.990 0.792 -0.756
ENSG00000104450 E013 8.5103901 0.0047321079 4.711763e-01 6.103262e-01 8 100194112 100194268 157 + 0.957 0.867 -0.340
ENSG00000104450 E014 2.8659632 0.0114376931 8.220720e-01 8.877967e-01 8 100194269 100194684 416 + 0.552 0.590 0.171
ENSG00000104450 E015 1.9304837 0.0130999403 5.359267e-01 6.677189e-01 8 100213087 100213089 3 + 0.452 0.334 -0.661
ENSG00000104450 E016 15.0949360 0.0082243751 1.128790e-02 3.184836e-02 8 100213090 100213428 339 + 1.219 0.931 -1.047
ENSG00000104450 E017 9.2168257 0.0024072931 4.531244e-01 5.938887e-01 8 100213819 100213918 100 + 0.991 0.900 -0.341
ENSG00000104450 E018 17.8983657 0.0017774417 2.685035e-01 4.069798e-01 8 100220279 100220431 153 + 1.256 1.155 -0.357
ENSG00000104450 E019 19.2921232 0.0009536502 6.848657e-01 7.877180e-01 8 100225173 100225339 167 + 1.269 1.235 -0.119
ENSG00000104450 E020 19.2737009 0.0015189452 5.725118e-01 6.986148e-01 8 100231156 100231288 133 + 1.269 1.221 -0.171
ENSG00000104450 E021 0.8136755 0.0145597483 4.378642e-01 5.797438e-01 8 100233171 100233410 240 + 0.190 0.334 1.074
ENSG00000104450 E022 23.5068471 0.0008273278 9.387410e-01 9.655390e-01 8 100233411 100233537 127 + 1.344 1.339 -0.017
ENSG00000104450 E023 23.5077804 0.0010492660 7.137098e-01 8.101221e-01 8 100239240 100239404 165 + 1.354 1.327 -0.095
ENSG00000104450 E024 28.4228515 0.0023247440 4.765335e-01 6.151560e-01 8 100240403 100240558 156 + 1.407 1.459 0.178
ENSG00000104450 E025 29.2289934 0.0007558497 6.126371e-01 7.315201e-01 8 100240559 100240771 213 + 1.423 1.459 0.125
ENSG00000104450 E026 58.8739667 0.0013027494 4.556512e-08 4.954861e-07 8 100240891 100241904 1014 + 1.642 1.892 0.845
ENSG00000104450 E027 2.0292650 0.0387328631 3.244933e-06 2.409622e-05 8 100259042 100259278 237 + 0.073 0.865 5.109