ENSG00000103994

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000564754 ENSG00000103994 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF106 protein_coding protein_coding 7.491507 4.843374 7.942379 0.6087561 0.133912 0.7123983 5.2608959 4.07342324 5.4695594 0.469168312 0.11083815 0.4242808 0.71522500 0.8431000 0.6889000 -0.1542 4.655714e-04 7.064387e-05 FALSE TRUE
ENST00000565380 ENSG00000103994 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF106 protein_coding protein_coding 7.491507 4.843374 7.942379 0.6087561 0.133912 0.7123983 0.5583769 0.05598611 0.6940153 0.007633849 0.08161362 3.4153726 0.06309167 0.0116000 0.0874000 0.0758 7.064387e-05 7.064387e-05 FALSE TRUE
MSTRG.10640.3 ENSG00000103994 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF106 protein_coding   7.491507 4.843374 7.942379 0.6087561 0.133912 0.7123983 1.4780576 0.62298577 1.4556404 0.121580680 0.08369010 1.2112862 0.19718333 0.1275333 0.1834333 0.0559 2.789781e-01 7.064387e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000103994 E001 251.1836209 0.0139801693 6.410372e-17 2.826218e-15 15 42412823 42413544 722 - 2.085 2.623 1.793
ENSG00000103994 E002 327.0520222 0.0038951389 1.472686e-17 7.063189e-16 15 42413545 42414593 1049 - 2.339 2.671 1.107
ENSG00000103994 E003 154.8976718 0.0002493412 2.145292e-12 4.984902e-11 15 42414594 42415120 527 - 2.063 2.315 0.842
ENSG00000103994 E004 19.7483096 0.0010534728 2.003381e-01 3.266471e-01 15 42415121 42415122 2 - 1.244 1.394 0.523
ENSG00000103994 E005 45.9015406 0.0005054552 6.198551e-05 3.412265e-04 15 42415123 42415294 172 - 1.539 1.798 0.881
ENSG00000103994 E006 39.3216020 0.0006201049 3.501634e-04 1.580817e-03 15 42415295 42415476 182 - 1.476 1.728 0.858
ENSG00000103994 E007 58.4074825 0.0049619317 9.919489e-02 1.892509e-01 15 42415477 42415739 263 - 1.702 1.844 0.482
ENSG00000103994 E008 265.5707344 0.0034824432 6.763812e-01 7.813768e-01 15 42415740 42417360 1621 - 2.404 2.444 0.132
ENSG00000103994 E009 34.6516328 0.0047098338 1.413471e-01 2.498813e-01 15 42417805 42417868 64 - 1.567 1.518 -0.168
ENSG00000103994 E010 38.4273379 0.0040317212 1.133537e-01 2.104066e-01 15 42417869 42417951 83 - 1.611 1.560 -0.173
ENSG00000103994 E011 33.4727735 0.0006118743 1.666076e-01 2.837783e-01 15 42421061 42421132 72 - 1.549 1.518 -0.105
ENSG00000103994 E012 23.4564514 0.0120739607 3.963873e-01 5.401580e-01 15 42421917 42421957 41 - 1.399 1.377 -0.077
ENSG00000103994 E013 25.2267192 0.0086942527 3.864658e-01 5.306419e-01 15 42421958 42421988 31 - 1.421 1.402 -0.063
ENSG00000103994 E014 31.8776731 0.0006116601 2.932763e-01 4.343195e-01 15 42422501 42422552 52 - 1.519 1.507 -0.041
ENSG00000103994 E015 38.5428181 0.0006892145 2.508063e-03 8.787590e-03 15 42422553 42422620 68 - 1.640 1.518 -0.415
ENSG00000103994 E016 36.6690046 0.0006876748 6.079995e-04 2.562371e-03 15 42423998 42424060 63 - 1.629 1.479 -0.516
ENSG00000103994 E017 0.2214452 0.0483087724 3.509609e-01   15 42424061 42424246 186 - 0.000 0.150 11.101
ENSG00000103994 E018 0.1472490 0.0435583041 6.697112e-01   15 42424277 42424833 557 - 0.100 0.000 -10.409
ENSG00000103994 E019 60.4754791 0.0089148970 2.617728e-03 9.119709e-03 15 42424834 42425025 192 - 1.839 1.701 -0.466
ENSG00000103994 E020 0.1482932 0.0416105949 3.534779e-01   15 42425472 42425781 310 - 0.000 0.149 11.140
ENSG00000103994 E021 42.6545507 0.0021807138 4.139096e-03 1.353224e-02 15 42428018 42428134 117 - 1.679 1.565 -0.391
ENSG00000103994 E022 41.7627345 0.0005088093 9.940680e-03 2.861259e-02 15 42435384 42435518 135 - 1.662 1.574 -0.302
ENSG00000103994 E023 42.6083493 0.0005275310 1.817775e-02 4.751467e-02 15 42437232 42437377 146 - 1.667 1.592 -0.255
ENSG00000103994 E024 29.5220107 0.0103176676 1.737238e-02 4.576121e-02 15 42438612 42438667 56 - 1.535 1.395 -0.483
ENSG00000103994 E025 30.8341830 0.0084074457 1.374095e-01 2.444738e-01 15 42439033 42439126 94 - 1.526 1.466 -0.203
ENSG00000103994 E026 52.5624596 0.0005369988 7.383966e-04 3.037061e-03 15 42439127 42439364 238 - 1.770 1.656 -0.387
ENSG00000103994 E027 41.7041040 0.0005319569 3.205211e-07 2.947372e-06 15 42439365 42439552 188 - 1.711 1.473 -0.812
ENSG00000103994 E028 42.2524034 0.0035231509 2.894031e-05 1.728454e-04 15 42439553 42439813 261 - 1.709 1.502 -0.705
ENSG00000103994 E029 2.1475884 0.0070974263 3.145319e-01 4.572405e-01 15 42441741 42442072 332 - 0.554 0.421 -0.658
ENSG00000103994 E030 65.9356380 0.0013028241 6.555548e-08 6.901138e-07 15 42442073 42442414 342 - 1.899 1.697 -0.684
ENSG00000103994 E031 20.8984505 0.0097718930 6.801522e-02 1.400757e-01 15 42444202 42444262 61 - 1.376 1.263 -0.394
ENSG00000103994 E032 33.8240415 0.0009607938 3.287242e-03 1.111294e-02 15 42444827 42444981 155 - 1.585 1.454 -0.448
ENSG00000103994 E033 20.0775287 0.0014204216 3.359533e-02 7.895857e-02 15 42446589 42446625 37 - 1.366 1.250 -0.404
ENSG00000103994 E034 18.0061242 0.0010336470 1.785810e-01 2.993764e-01 15 42446626 42446658 33 - 1.298 1.240 -0.203
ENSG00000103994 E035 90.1393465 0.0023982206 2.142513e-08 2.483891e-07 15 42448072 42448705 634 - 2.029 1.834 -0.655
ENSG00000103994 E036 149.8523429 0.0224488533 1.106347e-05 7.276850e-05 15 42449771 42451666 1896 - 2.271 2.002 -0.901
ENSG00000103994 E037 37.2659735 0.0005702320 2.559903e-14 7.925575e-13 15 42451667 42451954 288 - 1.717 1.289 -1.472
ENSG00000103994 E038 28.8890021 0.0248794532 9.453269e-07 7.881760e-06 15 42456958 42457158 201 - 1.616 1.162 -1.577
ENSG00000103994 E039 0.0000000       15 42457159 42457216 58 -      
ENSG00000103994 E040 0.0000000       15 42457217 42457556 340 -      
ENSG00000103994 E041 29.2294453 0.0006781377 1.775256e-13 4.877692e-12 15 42466053 42466114 62 - 1.627 1.150 -1.657
ENSG00000103994 E042 25.7368425 0.0007687453 2.769122e-09 3.785006e-08 15 42472236 42472321 86 - 1.552 1.162 -1.357
ENSG00000103994 E043 0.0000000       15 42490329 42490410 82 -      
ENSG00000103994 E044 18.8910806 0.0079707952 5.400644e-09 7.003462e-08 15 42490980 42491169 190 - 1.453 0.923 -1.894