ENSG00000103653

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000220003 ENSG00000103653 HEK293_OSMI2_2hA HEK293_TMG_2hB CSK protein_coding protein_coding 112.9178 170.5652 78.64599 8.898649 1.013936 -1.11678 15.149513 19.91746 12.63040 3.7190561 0.5756846 -0.6567150 0.1318833 0.1150667 0.1605667 0.0455000 3.741913e-01 3.374234e-08 FALSE TRUE
ENST00000439220 ENSG00000103653 HEK293_OSMI2_2hA HEK293_TMG_2hB CSK protein_coding protein_coding 112.9178 170.5652 78.64599 8.898649 1.013936 -1.11678 60.768457 115.96760 28.95096 3.4300820 2.3929549 -2.0016649 0.4965833 0.6817000 0.3687333 -0.3129667 3.374234e-08 3.374234e-08 FALSE TRUE
ENST00000567135 ENSG00000103653 HEK293_OSMI2_2hA HEK293_TMG_2hB CSK protein_coding retained_intron 112.9178 170.5652 78.64599 8.898649 1.013936 -1.11678 12.840122 8.90150 15.38088 1.5975893 1.7184222 0.7883357 0.1353583 0.0515000 0.1950667 0.1435667 4.689411e-08 3.374234e-08 FALSE FALSE
ENST00000569462 ENSG00000103653 HEK293_OSMI2_2hA HEK293_TMG_2hB CSK protein_coding protein_coding 112.9178 170.5652 78.64599 8.898649 1.013936 -1.11678 7.029309 1.93094 10.87765 0.5846328 1.0716544 2.4878646 0.0812500 0.0116000 0.1381333 0.1265333 1.716191e-07 3.374234e-08 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000103653 E001 14.9907704 4.499634e-02 4.666982e-03 1.500307e-02 15 74782080 74782088 9 + 1.372 1.012 -1.281
ENSG00000103653 E002 57.8270778 2.858592e-02 1.519059e-06 1.213140e-05 15 74782089 74782148 60 + 1.981 1.547 -1.466
ENSG00000103653 E003 112.9194527 2.671351e-02 2.557423e-05 1.546972e-04 15 74782149 74782492 344 + 2.211 1.874 -1.128
ENSG00000103653 E004 17.6823946 6.230725e-03 4.457131e-02 9.941018e-02 15 74782493 74782503 11 + 0.992 1.227 0.845
ENSG00000103653 E005 12.1217024 1.593396e-03 7.425494e-04 3.052419e-03 15 74782504 74782504 1 + 0.634 1.105 1.826
ENSG00000103653 E006 77.3453031 2.411233e-02 1.387396e-01 2.463583e-01 15 74782505 74782591 87 + 1.695 1.842 0.497
ENSG00000103653 E007 77.3910428 4.536037e-03 3.698757e-01 5.142775e-01 15 74782592 74782604 13 + 1.758 1.825 0.227
ENSG00000103653 E008 169.6530370 5.544665e-03 8.314056e-01 8.943180e-01 15 74782605 74782667 63 + 2.146 2.152 0.019
ENSG00000103653 E009 246.8889502 2.132272e-04 3.716481e-02 8.578553e-02 15 74782668 74782720 53 + 2.342 2.301 -0.138
ENSG00000103653 E010 4.8797229 4.184886e-02 3.736466e-01 5.180605e-01 15 74784435 74784442 8 + 0.814 0.681 -0.539
ENSG00000103653 E011 15.7498130 7.534088e-03 5.772469e-01 7.025284e-01 15 74784443 74784523 81 + 1.186 1.142 -0.156
ENSG00000103653 E012 0.3729606 2.817241e-02 4.292535e-01 5.719927e-01 15 74788544 74788577 34 + 0.191 0.076 -1.518
ENSG00000103653 E013 1.6325479 5.928284e-02 1.208492e-01 2.211582e-01 15 74789804 74789937 134 + 0.575 0.292 -1.524
ENSG00000103653 E014 1.5468372 5.312952e-02 1.171211e-01 2.158380e-01 15 74790817 74790946 130 + 0.575 0.291 -1.531
ENSG00000103653 E015 1.1781165 1.138823e-01 1.168244e-01 2.154304e-01 15 74793046 74793248 203 + 0.506 0.199 -1.921
ENSG00000103653 E016 5.5846482 1.054706e-01 1.229000e-01 2.240908e-01 15 74797914 74798211 298 + 0.424 0.796 1.667
ENSG00000103653 E017 5.9938433 2.799380e-03 9.493211e-01 9.722721e-01 15 74798212 74798232 21 + 0.775 0.778 0.010
ENSG00000103653 E018 517.2627737 2.649452e-04 1.692366e-05 1.067040e-04 15 74798233 74798312 80 + 2.685 2.618 -0.224
ENSG00000103653 E019 11.0715006 2.141448e-02 9.155568e-07 7.662212e-06 15 74798313 74798435 123 + 1.363 0.795 -2.073
ENSG00000103653 E020 18.8006947 3.773101e-02 2.254495e-05 1.381173e-04 15 74798436 74798614 179 + 1.532 1.047 -1.707
ENSG00000103653 E021 475.1773733 2.290106e-04 4.074382e-06 2.957053e-05 15 74798615 74798636 22 + 2.655 2.579 -0.251
ENSG00000103653 E022 449.5789316 1.815312e-03 2.336707e-03 8.265008e-03 15 74798637 74798651 15 + 2.627 2.559 -0.228
ENSG00000103653 E023 763.2068709 6.608649e-04 7.912288e-08 8.196408e-07 15 74798652 74798728 77 + 2.867 2.783 -0.281
ENSG00000103653 E024 11.7544303 1.697772e-03 6.160237e-03 1.903318e-02 15 74798729 74798825 97 + 1.216 0.949 -0.969
ENSG00000103653 E025 1041.7220195 9.659378e-05 2.751069e-12 6.286874e-11 15 74798826 74798938 113 + 2.994 2.919 -0.250
ENSG00000103653 E026 4.0869369 4.038266e-03 3.186784e-02 7.559718e-02 15 74798939 74799051 113 + 0.849 0.544 -1.278
ENSG00000103653 E027 9.4119031 3.614807e-03 9.188257e-03 2.675553e-02 15 74799178 74799271 94 + 1.136 0.855 -1.042
ENSG00000103653 E028 1370.8430396 6.500993e-05 1.714814e-03 6.328608e-03 15 74799272 74799491 220 + 3.071 3.050 -0.069
ENSG00000103653 E029 720.0038133 1.007407e-04 3.886323e-02 8.892901e-02 15 74800412 74800474 63 + 2.789 2.771 -0.061
ENSG00000103653 E030 433.1730375 1.207628e-03 4.196963e-01 5.629520e-01 15 74800475 74800488 14 + 2.559 2.554 -0.016
ENSG00000103653 E031 464.6731082 3.075513e-03 4.712957e-01 6.104245e-01 15 74800489 74800505 17 + 2.592 2.585 -0.021
ENSG00000103653 E032 14.3489637 1.240724e-03 3.734759e-06 2.737730e-05 15 74800506 74800680 175 + 1.383 0.976 -1.453
ENSG00000103653 E033 806.0457252 6.075736e-04 3.695221e-01 5.139329e-01 15 74800681 74800746 66 + 2.799 2.831 0.108
ENSG00000103653 E034 15.5591981 2.302390e-02 1.131230e-01 2.100895e-01 15 74800747 74800822 76 + 1.257 1.092 -0.590
ENSG00000103653 E035 712.5143977 9.909295e-05 8.808258e-01 9.275853e-01 15 74800823 74800851 29 + 2.762 2.773 0.039
ENSG00000103653 E036 946.7742866 1.046998e-04 6.605250e-01 7.691779e-01 15 74800852 74800922 71 + 2.879 2.899 0.068
ENSG00000103653 E037 5.2179351 3.443221e-03 3.307700e-01 4.743453e-01 15 74800923 74801011 89 + 0.814 0.685 -0.519
ENSG00000103653 E038 1021.2018703 1.007944e-04 6.081512e-01 7.279892e-01 15 74801012 74801102 91 + 2.912 2.933 0.070
ENSG00000103653 E039 899.4163350 4.148592e-04 8.512600e-01 9.077733e-01 15 74801522 74801595 74 + 2.865 2.877 0.041
ENSG00000103653 E040 16.2853394 2.558489e-02 3.209399e-01 4.641128e-01 15 74801630 74801694 65 + 1.245 1.136 -0.386
ENSG00000103653 E041 1334.3282963 1.178759e-04 6.484081e-02 1.347761e-01 15 74801695 74801890 196 + 3.015 3.052 0.122
ENSG00000103653 E042 10.5254683 4.783166e-03 6.431473e-01 7.557566e-01 15 74801891 74801996 106 + 0.940 1.009 0.256
ENSG00000103653 E043 719.9779945 9.446882e-04 3.541778e-07 3.227380e-06 15 74801997 74802083 87 + 2.674 2.801 0.424
ENSG00000103653 E044 2582.2589996 1.203316e-03 7.378742e-26 8.731297e-24 15 74802331 74803197 867 + 3.171 3.370 0.660