ENSG00000103647

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000261861 ENSG00000103647 HEK293_OSMI2_2hA HEK293_TMG_2hB CORO2B protein_coding protein_coding 0.6183172 1.137279 0.5369686 0.05042006 0.04641949 -1.068686 0.2497424 0.3944834 0.5369686 0.01575575 0.04641949 0.4353774 0.4157250 0.3470333 1 0.6529667 3.228925e-12 3.228925e-12 FALSE TRUE
ENST00000540068 ENSG00000103647 HEK293_OSMI2_2hA HEK293_TMG_2hB CORO2B protein_coding protein_coding 0.6183172 1.137279 0.5369686 0.05042006 0.04641949 -1.068686 0.3644961 0.7427951 0.0000000 0.03476668 0.00000000 -6.2341854 0.5635792 0.6529667 0 -0.6529667 3.335260e-12 3.228925e-12 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000103647 E001 1.802715 0.008001210 0.0001781629 0.0008700931 15 68578993 68579277 285 + 0.761 0.159 -3.427
ENSG00000103647 E002 0.000000       15 68616539 68616614 76 +      
ENSG00000103647 E003 0.000000       15 68631988 68632341 354 +      
ENSG00000103647 E004 5.773609 0.003344534 0.0463910916 0.1027431976 15 68645160 68645360 201 + 0.936 0.699 -0.933
ENSG00000103647 E005 6.021944 0.003038917 0.7666029952 0.8490586770 15 68695140 68695256 117 + 0.761 0.816 0.218
ENSG00000103647 E006 7.682603 0.002596694 0.5958748208 0.7178941231 15 68710732 68710881 150 + 0.828 0.909 0.312
ENSG00000103647 E007 8.309398 0.002594756 0.0344529115 0.0806270395 15 68711542 68711706 165 + 0.683 0.975 1.145
ENSG00000103647 E008 6.360461 0.027039019 0.6820024355 0.7855737545 15 68713925 68714041 117 + 0.761 0.834 0.288
ENSG00000103647 E009 5.982203 0.003052610 0.7666887440 0.8491089717 15 68714559 68714663 105 + 0.761 0.816 0.218
ENSG00000103647 E010 4.809462 0.004726707 0.3459022580 0.4900366055 15 68715215 68715311 97 + 0.796 0.679 -0.475
ENSG00000103647 E011 5.109171 0.003569897 0.0929243064 0.1797010236 15 68718698 68718810 113 + 0.885 0.679 -0.824
ENSG00000103647 E012 4.741520 0.004293713 0.5836931322 0.7077548679 15 68719144 68719234 91 + 0.761 0.699 -0.256
ENSG00000103647 E013 4.879443 0.034858873 0.8269413634 0.8911493932 15 68719413 68719552 140 + 0.683 0.738 0.228
ENSG00000103647 E014 9.073232 0.037202187 0.7942930005 0.8685708534 15 68725843 68726077 235 + 0.912 0.975 0.237
ENSG00000103647 E015 49.701860 0.075999375 0.7799482223 0.8585312408 15 68726078 68727751 1674 + 1.609 1.675 0.225
ENSG00000103647 E016 2.100342 0.007419465 0.6568247356 0.7662965929 15 68727752 68727806 55 + 0.386 0.477 0.481