ENSG00000103494

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000563746 ENSG00000103494 HEK293_OSMI2_2hA HEK293_TMG_2hB RPGRIP1L protein_coding protein_coding 5.216607 2.742166 7.947634 0.422595 0.1256164 1.531772 0.8290586 0.22406008 1.0333424 0.04159376 0.09246389 2.1562619 0.1291625 0.08933333 0.12986667 0.040533333 4.606201e-01 4.06546e-10 FALSE TRUE
ENST00000566096 ENSG00000103494 HEK293_OSMI2_2hA HEK293_TMG_2hB RPGRIP1L protein_coding protein_coding 5.216607 2.742166 7.947634 0.422595 0.1256164 1.531772 0.7640179 1.24103907 0.7959815 0.32117412 0.04626728 -0.6343083 0.2177458 0.44063333 0.10006667 -0.340566667 4.065460e-10 4.06546e-10   FALSE
ENST00000568009 ENSG00000103494 HEK293_OSMI2_2hA HEK293_TMG_2hB RPGRIP1L protein_coding processed_transcript 5.216607 2.742166 7.947634 0.422595 0.1256164 1.531772 0.3271019 0.28917919 0.6408996 0.09320071 0.14032318 1.1214252 0.0616000 0.11183333 0.08073333 -0.031100000 8.542672e-01 4.06546e-10 FALSE TRUE
ENST00000568653 ENSG00000103494 HEK293_OSMI2_2hA HEK293_TMG_2hB RPGRIP1L protein_coding protein_coding 5.216607 2.742166 7.947634 0.422595 0.1256164 1.531772 0.5623278 0.23322066 0.7441886 0.02950973 0.17914700 1.6326596 0.1095000 0.08713333 0.09316667 0.006033333 9.405648e-01 4.06546e-10 FALSE FALSE
ENST00000621565 ENSG00000103494 HEK293_OSMI2_2hA HEK293_TMG_2hB RPGRIP1L protein_coding protein_coding 5.216607 2.742166 7.947634 0.422595 0.1256164 1.531772 0.6916279 0.30773083 0.9952664 0.02608287 0.03642251 1.6617008 0.1357792 0.11453333 0.12523333 0.010700000 8.616043e-01 4.06546e-10 FALSE TRUE
ENST00000679110 ENSG00000103494 HEK293_OSMI2_2hA HEK293_TMG_2hB RPGRIP1L protein_coding nonsense_mediated_decay 5.216607 2.742166 7.947634 0.422595 0.1256164 1.531772 0.2387650 0.28840885 0.3787706 0.18517059 0.20265201 0.3816287 0.0515875 0.09480000 0.04823333 -0.046566667 8.592288e-01 4.06546e-10 TRUE FALSE
MSTRG.12665.4 ENSG00000103494 HEK293_OSMI2_2hA HEK293_TMG_2hB RPGRIP1L protein_coding   5.216607 2.742166 7.947634 0.422595 0.1256164 1.531772 1.1581808 0.07891501 2.4374002 0.07891501 0.30455826 4.7826792 0.1732542 0.03166667 0.30673333 0.275066667 2.331292e-02 4.06546e-10 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000103494 E001 0.7728905 0.0153787590 1.000000e+00 1.000000e+00 16 53598153 53598392 240 - 0.219 0.217 -0.019
ENSG00000103494 E002 1.2512830 0.0111736580 7.265752e-01 8.195547e-01 16 53598393 53598460 68 - 0.297 0.360 0.396
ENSG00000103494 E003 2.0534190 0.0348246770 7.654735e-01 8.482342e-01 16 53598461 53598518 58 - 0.421 0.469 0.250
ENSG00000103494 E004 2.5100416 0.0062105001 9.103704e-01 9.470192e-01 16 53598519 53599253 735 - 0.495 0.468 -0.134
ENSG00000103494 E005 51.7310840 0.0004449334 2.578054e-02 6.349101e-02 16 53599254 53601780 2527 - 1.624 1.738 0.384
ENSG00000103494 E006 17.8961930 0.0011087874 6.984357e-02 1.431176e-01 16 53601781 53602004 224 - 1.169 1.323 0.542
ENSG00000103494 E007 6.5095122 0.0083446124 3.585902e-01 5.029915e-01 16 53602005 53602023 19 - 0.839 0.689 -0.602
ENSG00000103494 E008 24.4276394 0.0009171099 5.874831e-01 7.107622e-01 16 53602024 53602188 165 - 1.325 1.361 0.126
ENSG00000103494 E009 0.7362571 0.0476031726 3.247582e-01 4.681764e-01 16 53605395 53605480 86 - 0.174 0.359 1.388
ENSG00000103494 E010 26.8029270 0.0007644900 2.067504e-01 3.346655e-01 16 53605481 53605614 134 - 1.351 1.439 0.305
ENSG00000103494 E011 20.9357727 0.0044219736 5.622311e-01 6.898846e-01 16 53610967 53611051 85 - 1.264 1.309 0.158
ENSG00000103494 E012 25.2488498 0.0007460208 4.206152e-01 5.638163e-01 16 53619025 53619208 184 - 1.370 1.295 -0.260
ENSG00000103494 E013 4.0672605 0.0294669490 2.274139e-01 3.593114e-01 16 53622219 53622356 138 - 0.597 0.790 0.808
ENSG00000103494 E014 0.5985731 0.0268144863 8.043901e-01 8.755670e-01 16 53631252 53631338 87 - 0.174 0.216 0.393
ENSG00000103494 E015 2.4045080 0.0062750731 2.280210e-01 3.600604e-01 16 53635441 53635623 183 - 0.421 0.627 0.981
ENSG00000103494 E016 15.7001842 0.0011275384 6.429414e-01 7.556039e-01 16 53636439 53636512 74 - 1.144 1.183 0.136
ENSG00000103494 E017 30.4248025 0.0159315618 3.987923e-01 5.425325e-01 16 53637695 53637854 160 - 1.443 1.352 -0.313
ENSG00000103494 E018 19.5537709 0.0017533112 5.663838e-02 1.208302e-01 16 53638310 53638411 102 - 1.279 1.079 -0.712
ENSG00000103494 E019 23.5825360 0.0008514422 3.569007e-01 5.013069e-01 16 53641033 53641116 84 - 1.339 1.251 -0.307
ENSG00000103494 E020 27.7156899 0.0009041937 4.685183e-01 6.079285e-01 16 53641285 53641475 191 - 1.402 1.336 -0.229
ENSG00000103494 E021 0.0000000       16 53641476 53642170 695 -      
ENSG00000103494 E022 33.8141918 0.0006038119 1.089231e-02 3.090723e-02 16 53645625 53646003 379 - 1.513 1.309 -0.705
ENSG00000103494 E023 19.2011350 0.0018720285 1.504790e-03 5.651712e-03 16 53648964 53649115 152 - 1.297 0.943 -1.279
ENSG00000103494 E024 42.0070368 0.0270874205 3.963366e-04 1.760272e-03 16 53652535 53652987 453 - 1.626 1.225 -1.385
ENSG00000103494 E025 21.1182904 0.0009197098 3.388105e-02 7.951863e-02 16 53656472 53656589 118 - 1.315 1.102 -0.756
ENSG00000103494 E026 23.6575866 0.0009165075 4.834014e-06 3.454506e-05 16 53657453 53657632 180 - 1.399 0.909 -1.760
ENSG00000103494 E027 14.6828632 0.0012839429 1.092556e-02 3.098293e-02 16 53658414 53658464 51 - 1.183 0.873 -1.140
ENSG00000103494 E028 18.7762381 0.0010187708 1.035456e-02 2.963695e-02 16 53658772 53658878 107 - 1.279 1.002 -0.993
ENSG00000103494 E029 2.0766013 0.0069790576 5.834101e-01 7.075339e-01 16 53658879 53660545 1667 - 0.472 0.360 -0.606
ENSG00000103494 E030 0.1817044 0.0424209411 1.052825e-01   16 53662882 53663411 530 - 0.000 0.217 10.467
ENSG00000103494 E031 15.0732397 0.0013487823 4.099237e-02 9.286777e-02 16 53664870 53665009 140 - 1.183 0.943 -0.877
ENSG00000103494 E032 8.4113192 0.0088389094 3.030545e-02 7.256690e-02 16 53671510 53671550 41 - 0.971 0.627 -1.370
ENSG00000103494 E033 9.0189621 0.0319814140 2.962109e-01 4.374175e-01 16 53671551 53671583 33 - 0.971 0.792 -0.684
ENSG00000103494 E034 17.2187293 0.0011984705 2.994135e-03 1.024676e-02 16 53672870 53673016 147 - 1.252 0.909 -1.246
ENSG00000103494 E035 21.0840557 0.0009447596 6.447224e-06 4.476151e-05 16 53675017 53675122 106 - 1.351 0.834 -1.883
ENSG00000103494 E036 26.2070355 0.0044615060 1.644395e-08 1.947645e-07 16 53686433 53686576 144 - 1.453 0.791 -2.402
ENSG00000103494 E037 19.5086043 0.0090357304 2.476818e-06 1.888840e-05 16 53687863 53687965 103 - 1.329 0.689 -2.388
ENSG00000103494 E038 22.8106863 0.0020935576 1.537256e-06 1.225761e-05 16 53692066 53692226 161 - 1.385 0.834 -1.998
ENSG00000103494 E039 22.8542010 0.1186999156 6.918954e-03 2.101443e-02 16 53692227 53692364 138 - 1.384 0.852 -1.926
ENSG00000103494 E040 1.9134406 0.0081333665 1.793773e-03 6.579887e-03 16 53693312 53693426 115 - 0.219 0.743 2.790
ENSG00000103494 E041 15.5586002 0.0196956787 3.445104e-05 2.017885e-04 16 53693427 53694244 818 - 1.008 1.454 1.580
ENSG00000103494 E042 3.6701530 0.0461330917 3.257807e-02 7.696021e-02 16 53694245 53694742 498 - 0.692 0.216 -2.605
ENSG00000103494 E043 20.2410792 0.0075834943 4.855250e-02 1.065982e-01 16 53694743 53695458 716 - 1.219 1.394 0.612
ENSG00000103494 E044 11.3247830 0.0089446121 5.608655e-10 8.712225e-09 16 53695631 53696150 520 - 0.784 1.426 2.339
ENSG00000103494 E045 36.0056333 0.0045777394 5.393736e-06 3.810797e-05 16 53696151 53696212 62 - 1.413 1.714 1.029
ENSG00000103494 E046 46.1418280 0.0016476536 1.380322e-11 2.811503e-10 16 53696213 53696295 83 - 1.491 1.857 1.244
ENSG00000103494 E047 39.5525513 0.0005446265 6.675811e-08 7.016824e-07 16 53700639 53700726 88 - 1.445 1.753 1.049
ENSG00000103494 E048 20.6988965 0.0020224888 5.727099e-06 4.022175e-05 16 53700727 53700730 4 - 1.159 1.514 1.237
ENSG00000103494 E049 0.2934659 0.0288220907 3.104083e-01   16 53703228 53703261 34 - 0.066 0.216 1.978
ENSG00000103494 E050 0.8814298 0.0315473806 4.562309e-01 5.966806e-01 16 53703555 53703802 248 - 0.219 0.359 0.976
ENSG00000103494 E051 21.2631265 0.0009487146 3.393374e-06 2.510601e-05 16 53703803 53703938 136 - 1.169 1.523 1.232