ENSG00000103449

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000251020 ENSG00000103449 HEK293_OSMI2_2hA HEK293_TMG_2hB SALL1 protein_coding protein_coding 8.292457 7.684964 8.10857 0.3922813 0.294876 0.07731105 2.5305243 3.067098 1.9293007 0.9729143 0.6582881 -0.6660338 0.30500833 0.3929000 0.24170000 -0.15120000 0.5473809422 0.0009812275 FALSE TRUE
ENST00000440970 ENSG00000103449 HEK293_OSMI2_2hA HEK293_TMG_2hB SALL1 protein_coding protein_coding 8.292457 7.684964 8.10857 0.3922813 0.294876 0.07731105 2.5771842 1.246787 2.9787830 0.6251071 0.3099649 1.2498178 0.31367083 0.1692667 0.36676667 0.19750000 0.5652270455 0.0009812275 FALSE TRUE
ENST00000566102 ENSG00000103449 HEK293_OSMI2_2hA HEK293_TMG_2hB SALL1 protein_coding protein_coding 8.292457 7.684964 8.10857 0.3922813 0.294876 0.07731105 2.3828674 2.869459 1.8978270 0.1328412 0.1705827 -0.5938672 0.28900417 0.3744333 0.23323333 -0.14120000 0.0025489421 0.0009812275 FALSE TRUE
ENST00000570206 ENSG00000103449 HEK293_OSMI2_2hA HEK293_TMG_2hB SALL1 protein_coding protein_coding 8.292457 7.684964 8.10857 0.3922813 0.294876 0.07731105 0.2610964 0.000000 0.7874319 0.0000000 0.3911877 6.3172894 0.02990417 0.0000000 0.09413333 0.09413333 0.0009812275 0.0009812275 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000103449 E001 1.3264216 0.010864776 1.411509e-01 2.496161e-01 16 51135982 51135995 14 - 0.224 0.460 1.482
ENSG00000103449 E002 4.2897291 0.056978110 1.208450e-02 3.373731e-02 16 51135996 51136018 23 - 0.429 0.847 1.836
ENSG00000103449 E003 150.5357686 0.008203991 1.949583e-07 1.872175e-06 16 51136019 51137063 1045 - 2.053 2.252 0.664
ENSG00000103449 E004 26.0318831 0.001476197 1.720421e-01 2.908596e-01 16 51137064 51137111 48 - 1.403 1.452 0.170
ENSG00000103449 E005 126.1610188 0.001324447 7.304696e-04 3.008637e-03 16 51137112 51137552 441 - 2.063 2.129 0.222
ENSG00000103449 E006 1.4663855 0.010701814 3.448936e-01 4.889896e-01 16 51138187 51138687 501 - 0.480 0.289 -1.100
ENSG00000103449 E007 495.2760195 0.005192819 5.232514e-08 5.619524e-07 16 51138688 51142145 3458 - 2.763 2.626 -0.455
ENSG00000103449 E008 1.3651183 0.009830482 4.543461e-01 5.949713e-01 16 51143204 51143408 205 - 0.303 0.410 0.636
ENSG00000103449 E009 0.2966881 0.029078516 2.094314e-01   16 51150381 51150597 217 - 0.224 0.000 -10.410
ENSG00000103449 E010 19.5955432 0.030370314 9.853071e-02 1.882494e-01 16 51151166 51151258 93 - 1.415 1.189 -0.792
ENSG00000103449 E011 5.4872953 0.178656267 6.174932e-01 7.352851e-01 16 51151259 51151367 109 - 0.872 0.727 -0.572
ENSG00000103449 E012 1.4653664 0.539970660 7.015844e-01 8.006859e-01 16 51152186 51152297 112 - 0.429 0.357 -0.404
ENSG00000103449 E013 1.7477602 0.197841600 1.277340e-01 2.310084e-01 16 51152298 51152334 37 - 0.607 0.214 -2.255