ENSG00000103260

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000219542 ENSG00000103260 HEK293_OSMI2_2hA HEK293_TMG_2hB METRN protein_coding protein_coding 58.89882 115.5657 24.24616 5.483146 0.7564393 -2.252415 18.610393 25.654504 10.193326 7.1137999 0.09471076 -1.3307349 0.29914167 0.2183000 0.4214333 0.2031333 8.122172e-02 1.009779e-13 FALSE FALSE
ENST00000568223 ENSG00000103260 HEK293_OSMI2_2hA HEK293_TMG_2hB METRN protein_coding protein_coding 58.89882 115.5657 24.24616 5.483146 0.7564393 -2.252415 2.911801 3.721139 2.441938 0.6834838 0.07512069 -0.6056936 0.05385833 0.0320000 0.1007000 0.0687000 2.066647e-06 1.009779e-13 FALSE TRUE
ENST00000570132 ENSG00000103260 HEK293_OSMI2_2hA HEK293_TMG_2hB METRN protein_coding nonsense_mediated_decay 58.89882 115.5657 24.24616 5.483146 0.7564393 -2.252415 26.023400 56.233496 9.030887 3.1345016 0.38022100 -2.6371500 0.42354167 0.4867333 0.3722333 -0.1145000 7.165659e-04 1.009779e-13   FALSE
MSTRG.11745.2 ENSG00000103260 HEK293_OSMI2_2hA HEK293_TMG_2hB METRN protein_coding   58.89882 115.5657 24.24616 5.483146 0.7564393 -2.252415 6.340071 22.273635 0.000000 5.7456119 0.00000000 -11.1217689 0.11612500 0.1965667 0.0000000 -0.1965667 1.009779e-13 1.009779e-13 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000103260 E001 20.73319 0.0009789755 9.526523e-49 5.371343e-46 16 715118 715227 110 + 1.848 0.736 -3.966
ENSG00000103260 E002 18.36230 0.0013110561 3.828599e-38 1.175554e-35 16 715228 715236 9 + 1.770 0.736 -3.702
ENSG00000103260 E003 59.83733 0.0377023302 1.199021e-05 7.823344e-05 16 715237 715337 101 + 1.980 1.552 -1.449
ENSG00000103260 E004 81.07620 0.0336407903 1.734923e-02 4.571102e-02 16 715338 715393 56 + 1.957 1.749 -0.698
ENSG00000103260 E005 83.22128 0.0472053954 1.085134e-01 2.033609e-01 16 715584 715640 57 + 1.927 1.775 -0.512
ENSG00000103260 E006 150.38638 0.0434815705 8.898356e-03 2.602737e-02 16 715641 715875 235 + 2.244 2.009 -0.786
ENSG00000103260 E007 281.87173 0.0043806225 7.960981e-01 8.698218e-01 16 715876 715984 109 + 2.332 2.328 -0.011
ENSG00000103260 E008 27.34333 0.0089081082 4.679225e-03 1.503758e-02 16 715985 716351 367 + 1.521 1.282 -0.824
ENSG00000103260 E009 24.52025 0.0341829774 9.329246e-02 1.802694e-01 16 716352 716426 75 + 1.437 1.254 -0.637
ENSG00000103260 E010 33.09338 0.0006193348 1.796520e-05 1.126114e-04 16 716427 716573 147 + 1.632 1.344 -0.988
ENSG00000103260 E011 35.51272 0.0016755029 5.190866e-07 4.576692e-06 16 716574 716715 142 + 1.692 1.363 -1.127
ENSG00000103260 E012 28.71317 0.0083406645 5.315567e-05 2.975559e-04 16 716716 716932 217 + 1.605 1.278 -1.126
ENSG00000103260 E013 198.21218 0.0002696211 1.053257e-02 3.005021e-02 16 716933 716942 10 + 2.100 2.187 0.293
ENSG00000103260 E014 350.52289 0.0001517562 1.939166e-06 1.512803e-05 16 716943 716992 50 + 2.318 2.439 0.404
ENSG00000103260 E015 230.96148 0.0001906885 1.312653e-05 8.480651e-05 16 716993 717070 78 + 2.123 2.262 0.464
ENSG00000103260 E016 607.79606 0.0016906892 8.549109e-09 1.067213e-07 16 717071 717135 65 + 2.536 2.686 0.499
ENSG00000103260 E017 947.80510 0.0043199027 1.131461e-08 1.381133e-07 16 717136 717423 288 + 2.713 2.883 0.564
ENSG00000103260 E018 227.65280 0.0011495562 1.590286e-12 3.767472e-11 16 717424 719655 2232 + 2.403 2.194 -0.698