ENSG00000103257

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000261622 ENSG00000103257 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC7A5 protein_coding protein_coding 141.18 166.4071 121.4242 10.3414 1.002619 -0.4546289 76.866509 67.5148289 80.646651 12.5066547 1.152656 0.2563754 0.54718333 0.3995 0.66443333 0.26493333 1.719808e-03 2.393693e-17 FALSE TRUE
ENST00000565644 ENSG00000103257 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC7A5 protein_coding protein_coding 141.18 166.4071 121.4242 10.3414 1.002619 -0.4546289 58.048539 97.8910168 27.568723 4.2111028 1.512321 -1.8277682 0.40623750 0.5944 0.22686667 -0.36753333 4.487314e-07 2.393693e-17 FALSE TRUE
MSTRG.13212.4 ENSG00000103257 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC7A5 protein_coding   141.18 166.4071 121.4242 10.3414 1.002619 -0.4546289 2.480147 0.1526532 7.241017 0.0701851 1.456069 5.4783121 0.01862083 0.0009 0.05956667 0.05866667 2.393693e-17 2.393693e-17 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000103257 E001 11041.677327 0.0040252795 3.103973e-19 1.814933e-17 16 87830023 87832731 2709 - 3.863 4.136 0.908
ENSG00000103257 E002 1379.237149 0.0017987144 1.174883e-01 2.163556e-01 16 87832732 87833025 294 - 3.085 3.168 0.278
ENSG00000103257 E003 1079.150969 0.0007707557 2.109651e-10 3.518816e-09 16 87834414 87834522 109 - 3.023 3.031 0.029
ENSG00000103257 E004 956.543363 0.0004353525 3.695783e-18 1.897892e-16 16 87834523 87834591 69 - 2.982 2.967 -0.050
ENSG00000103257 E005 1442.346369 0.0006326157 2.519748e-19 1.493146e-17 16 87836498 87836647 150 - 3.162 3.146 -0.056
ENSG00000103257 E006 2.405538 0.0103457054 5.187300e-01 6.529431e-01 16 87836648 87836805 158 - 0.530 0.502 -0.134
ENSG00000103257 E007 1091.839420 0.0002927408 8.894257e-23 7.704574e-21 16 87837845 87837941 97 - 3.040 3.024 -0.051
ENSG00000103257 E008 1013.337846 0.0006012129 9.613671e-22 7.427737e-20 16 87838714 87838817 104 - 3.020 2.983 -0.124
ENSG00000103257 E009 1095.471052 0.0007932884 3.525021e-20 2.292498e-18 16 87839702 87839825 124 - 3.055 3.016 -0.131
ENSG00000103257 E010 683.845110 0.0026828475 3.762365e-06 2.755195e-05 16 87840429 87840473 45 - 2.836 2.824 -0.038
ENSG00000103257 E011 933.696447 0.0037494288 7.703924e-07 6.558600e-06 16 87841050 87841155 106 - 2.984 2.950 -0.114
ENSG00000103257 E012 3.273173 0.0534825088 4.505253e-01 5.916242e-01 16 87843643 87843819 177 - 0.484 0.704 0.986
ENSG00000103257 E013 832.827430 0.0055264694 1.221175e-04 6.235122e-04 16 87851724 87851849 126 - 2.928 2.906 -0.074
ENSG00000103257 E014 0.147249 0.0431168415 3.495712e-01   16 87863590 87863639 50 - 0.127 0.000 -8.324
ENSG00000103257 E015 1164.406386 0.0095584789 1.346682e-06 1.087767e-05 16 87868885 87869507 623 - 3.117 3.015 -0.339