ENSG00000103254

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000564640 ENSG00000103254 HEK293_OSMI2_2hA HEK293_TMG_2hB ANTKMT protein_coding retained_intron 62.43496 120.3908 29.16146 5.888059 0.8434152 -2.045216 4.277312 5.397622 3.239102 0.8336331 0.5532151 -0.7349534 0.08817083 0.04446667 0.11050000 0.06603333 1.707901e-03 3.28627e-08 FALSE FALSE
ENST00000566437 ENSG00000103254 HEK293_OSMI2_2hA HEK293_TMG_2hB ANTKMT protein_coding retained_intron 62.43496 120.3908 29.16146 5.888059 0.8434152 -2.045216 12.073561 28.303134 3.699150 1.0737713 0.1721464 -2.9323109 0.17827083 0.23533333 0.12726667 -0.10806667 3.286270e-08 3.28627e-08 TRUE TRUE
ENST00000566525 ENSG00000103254 HEK293_OSMI2_2hA HEK293_TMG_2hB ANTKMT protein_coding retained_intron 62.43496 120.3908 29.16146 5.888059 0.8434152 -2.045216 2.059085 1.857105 2.492135 0.5610075 0.2384270 0.4223566 0.03913333 0.01506667 0.08513333 0.07006667 3.931126e-05 3.28627e-08 TRUE FALSE
ENST00000568916 ENSG00000103254 HEK293_OSMI2_2hA HEK293_TMG_2hB ANTKMT protein_coding protein_coding 62.43496 120.3908 29.16146 5.888059 0.8434152 -2.045216 6.122059 13.747901 2.614109 0.8724416 0.2357837 -2.3903611 0.09180000 0.11536667 0.08956667 -0.02580000 4.461588e-01 3.28627e-08 FALSE FALSE
ENST00000569529 ENSG00000103254 HEK293_OSMI2_2hA HEK293_TMG_2hB ANTKMT protein_coding protein_coding 62.43496 120.3908 29.16146 5.888059 0.8434152 -2.045216 15.051665 27.618979 8.339709 3.0971629 0.3592712 -1.7263844 0.23205000 0.22810000 0.28650000 0.05840000 2.324218e-01 3.28627e-08 FALSE TRUE
MSTRG.11746.9 ENSG00000103254 HEK293_OSMI2_2hA HEK293_TMG_2hB ANTKMT protein_coding   62.43496 120.3908 29.16146 5.888059 0.8434152 -2.045216 18.205904 33.420556 7.109301 4.6884364 0.2183439 -2.2313599 0.27357917 0.27530000 0.24383333 -0.03146667 7.309455e-01 3.28627e-08 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000103254 E001 20.034960 0.0485677376 2.708170e-07 2.528111e-06 16 719873 720580 708 + 1.669 0.985 -2.399
ENSG00000103254 E002 11.395280 0.0015595906 8.661146e-09 1.080061e-07 16 720581 720812 232 + 1.381 0.799 -2.123
ENSG00000103254 E003 12.327601 0.0049516688 5.064528e-11 9.402779e-10 16 720813 721096 284 + 1.455 0.799 -2.377
ENSG00000103254 E004 4.335567 0.0167424132 1.089662e-04 5.633018e-04 16 721097 721141 45 + 1.026 0.451 -2.395
ENSG00000103254 E005 2.643470 0.0061558316 2.231793e-03 7.941819e-03 16 721142 721145 4 + 0.830 0.334 -2.316
ENSG00000103254 E006 101.649012 0.0155780593 4.468344e-07 3.991674e-06 16 721146 721224 79 + 2.152 1.806 -1.161
ENSG00000103254 E007 148.086567 0.0177116974 1.230626e-07 1.228531e-06 16 721225 721436 212 + 2.319 1.966 -1.182
ENSG00000103254 E008 21.663458 0.0200798406 2.515830e-09 3.464034e-08 16 721437 721516 80 + 1.643 1.053 -2.058
ENSG00000103254 E009 109.153613 0.0078403719 2.086980e-01 3.369400e-01 16 721517 721597 81 + 1.976 1.906 -0.236
ENSG00000103254 E010 257.138995 0.0072872420 4.893716e-01 6.268084e-01 16 721598 721671 74 + 2.313 2.286 -0.090
ENSG00000103254 E011 277.595850 0.0055847928 8.380875e-01 8.987601e-01 16 721672 721702 31 + 2.329 2.323 -0.019
ENSG00000103254 E012 621.134918 0.0003184203 8.984106e-05 4.742301e-04 16 721801 721941 141 + 2.614 2.681 0.222
ENSG00000103254 E013 452.701437 0.0015215130 2.471134e-05 1.501199e-04 16 721942 722083 142 + 2.444 2.553 0.366
ENSG00000103254 E014 320.785336 0.0014465489 5.881077e-04 2.490115e-03 16 722084 722134 51 + 2.302 2.402 0.335
ENSG00000103254 E015 62.141153 0.0084560700 1.631327e-01 2.792360e-01 16 722135 722216 82 + 1.754 1.657 -0.328
ENSG00000103254 E016 61.976886 0.0038620559 2.681257e-02 6.560366e-02 16 722217 722308 92 + 1.787 1.649 -0.468
ENSG00000103254 E017 820.078963 0.0072796460 7.335139e-04 3.019797e-03 16 722309 722590 282 + 2.695 2.815 0.399