ENSG00000103253

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000389703 ENSG00000103253 HEK293_OSMI2_2hA HEK293_TMG_2hB HAGHL protein_coding protein_coding 11.00751 16.84799 6.486293 0.9713735 0.4419497 -1.375744 2.3407005 4.7731547 1.07020224 0.23549718 0.61896040 -2.1466610 0.20184167 0.28376667 0.165566667 -0.11820000 0.677763759 0.0008326644 FALSE  
ENST00000561546 ENSG00000103253 HEK293_OSMI2_2hA HEK293_TMG_2hB HAGHL protein_coding protein_coding 11.00751 16.84799 6.486293 0.9713735 0.4419497 -1.375744 0.8817182 0.5337199 0.66705898 0.53371993 0.38715102 0.3164178 0.09371250 0.02840000 0.104433333 0.07603333 0.484733179 0.0008326644 FALSE  
ENST00000561561 ENSG00000103253 HEK293_OSMI2_2hA HEK293_TMG_2hB HAGHL protein_coding nonsense_mediated_decay 11.00751 16.84799 6.486293 0.9713735 0.4419497 -1.375744 0.5000033 0.0000000 0.00000000 0.00000000 0.00000000 0.0000000 0.05496667 0.00000000 0.000000000 0.00000000   0.0008326644    
ENST00000564537 ENSG00000103253 HEK293_OSMI2_2hA HEK293_TMG_2hB HAGHL protein_coding protein_coding 11.00751 16.84799 6.486293 0.9713735 0.4419497 -1.375744 0.7816107 1.5138741 0.74631696 0.36917481 0.46196399 -1.0106808 0.08360417 0.08870000 0.126100000 0.03740000 0.952254310 0.0008326644 FALSE  
ENST00000567696 ENSG00000103253 HEK293_OSMI2_2hA HEK293_TMG_2hB HAGHL protein_coding retained_intron 11.00751 16.84799 6.486293 0.9713735 0.4419497 -1.375744 0.6375137 0.6749812 0.54303293 0.11412351 0.03085842 -0.3086991 0.06474583 0.03960000 0.084466667 0.04486667 0.018105151 0.0008326644 FALSE  
ENST00000569143 ENSG00000103253 HEK293_OSMI2_2hA HEK293_TMG_2hB HAGHL protein_coding retained_intron 11.00751 16.84799 6.486293 0.9713735 0.4419497 -1.375744 0.5664926 0.9016894 0.15516524 0.03771446 0.15516524 -2.4646324 0.05146250 0.05403333 0.022166667 -0.03186667 0.303197707 0.0008326644    
ENST00000569385 ENSG00000103253 HEK293_OSMI2_2hA HEK293_TMG_2hB HAGHL protein_coding nonsense_mediated_decay 11.00751 16.84799 6.486293 0.9713735 0.4419497 -1.375744 1.3631310 2.0797963 0.41325641 0.10900078 0.41325641 -2.3037585 0.10868333 0.12443333 0.059066667 -0.06536667 0.244215420 0.0008326644 FALSE  
MSTRG.11748.16 ENSG00000103253 HEK293_OSMI2_2hA HEK293_TMG_2hB HAGHL protein_coding   11.00751 16.84799 6.486293 0.9713735 0.4419497 -1.375744 0.4414817 1.0574246 0.04355575 0.13254910 0.04355575 -4.3169488 0.03519167 0.06386667 0.006233333 -0.05763333 0.006857422 0.0008326644 FALSE  
MSTRG.11748.5 ENSG00000103253 HEK293_OSMI2_2hA HEK293_TMG_2hB HAGHL protein_coding   11.00751 16.84799 6.486293 0.9713735 0.4419497 -1.375744 0.5759893 2.2083953 0.35679781 0.22995922 0.35679781 -2.5964595 0.05183333 0.13050000 0.052033333 -0.07846667 0.215813501 0.0008326644 FALSE  
MSTRG.11748.8 ENSG00000103253 HEK293_OSMI2_2hA HEK293_TMG_2hB HAGHL protein_coding   11.00751 16.84799 6.486293 0.9713735 0.4419497 -1.375744 0.9581821 1.2329948 0.64828638 0.63768505 0.32436055 -0.9170331 0.07730417 0.07763333 0.093633333 0.01600000 0.985297475 0.0008326644 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000103253 E001 11.212205 0.0150994793 2.097018e-06 1.623912e-05 16 726561 726633 73 + 1.355 0.828 -1.916
ENSG00000103253 E002 1.652229 0.0563664861 3.617793e-02 8.392785e-02 16 726936 726957 22 + 0.636 0.247 -2.116
ENSG00000103253 E003 1.618818 0.1032524345 4.845402e-02 1.064275e-01 16 726958 727009 52 + 0.636 0.247 -2.119
ENSG00000103253 E004 2.708481 0.0090350401 1.928371e-02 4.995271e-02 16 727010 727105 96 + 0.778 0.402 -1.712
ENSG00000103253 E005 3.592087 0.0047877390 1.623819e-02 4.324987e-02 16 727106 727118 13 + 0.852 0.491 -1.540
ENSG00000103253 E006 10.526928 0.0019428919 8.958247e-01 9.374301e-01 16 727119 727140 22 + 0.970 0.982 0.046
ENSG00000103253 E007 4.257969 0.0614322673 8.187944e-01 8.855038e-01 16 727141 727175 35 + 0.689 0.648 -0.174
ENSG00000103253 E008 1.402697 0.0095011159 6.114841e-02 1.285654e-01 16 727176 727245 70 + 0.577 0.246 -1.863
ENSG00000103253 E009 2.395986 0.0062374548 1.701385e-01 2.883680e-01 16 727246 727261 16 + 0.636 0.403 -1.124
ENSG00000103253 E010 6.854933 0.0519906012 8.871269e-01 9.318537e-01 16 727262 727274 13 + 0.778 0.814 0.143
ENSG00000103253 E011 15.632277 0.0026736817 6.456670e-02 1.343183e-01 16 727275 727292 18 + 0.970 1.169 0.726
ENSG00000103253 E012 23.568806 0.0010771889 1.045673e-02 2.987884e-02 16 727293 727317 25 + 1.121 1.350 0.809
ENSG00000103253 E013 35.814707 0.0005960779 3.917669e-03 1.291216e-02 16 727318 727345 28 + 1.310 1.520 0.724
ENSG00000103253 E014 47.743184 0.0004948997 4.379444e-03 1.420453e-02 16 727346 727404 59 + 1.459 1.636 0.605
ENSG00000103253 E015 27.618971 0.0007317817 1.173221e-01 2.161274e-01 16 727405 727411 7 + 1.273 1.396 0.427
ENSG00000103253 E016 61.830764 0.0005431488 1.265997e-01 2.294125e-01 16 727412 727481 70 + 1.652 1.730 0.266
ENSG00000103253 E017 41.084510 0.0005723799 5.522384e-01 6.815452e-01 16 727482 727484 3 + 1.514 1.549 0.120
ENSG00000103253 E018 86.842568 0.0004184963 1.365117e-01 2.432077e-01 16 727485 727614 130 + 1.808 1.871 0.213
ENSG00000103253 E019 8.446626 0.0020060922 1.339136e-02 3.682454e-02 16 727615 727679 65 + 1.102 0.815 -1.073
ENSG00000103253 E020 5.514726 0.0180888461 9.969332e-03 2.868778e-02 16 727680 727738 59 + 0.995 0.629 -1.446
ENSG00000103253 E021 11.448308 0.0039015410 6.486148e-04 2.711165e-03 16 727739 727964 226 + 1.260 0.907 -1.282
ENSG00000103253 E022 47.846663 0.0004981476 7.577215e-01 8.424393e-01 16 727965 728029 65 + 1.594 1.610 0.052
ENSG00000103253 E023 6.677220 0.0025237156 7.723146e-01 8.530257e-01 16 728030 728115 86 + 0.778 0.815 0.149
ENSG00000103253 E024 40.416312 0.0006021774 6.996686e-01 7.993132e-01 16 728116 728233 118 + 1.556 1.529 -0.090
ENSG00000103253 E025 3.137641 0.0076684070 9.282175e-02 1.795357e-01 16 728234 728315 82 + 0.736 0.464 -1.215
ENSG00000103253 E026 19.197120 0.0084289984 6.647612e-01 7.725345e-01 16 728316 728351 36 + 1.247 1.205 -0.147
ENSG00000103253 E027 19.255625 0.0201973034 4.634702e-01 6.032666e-01 16 728352 728424 73 + 1.273 1.200 -0.259
ENSG00000103253 E028 8.996831 0.0019304279 6.629990e-01 7.711677e-01 16 728425 728503 79 + 0.884 0.936 0.197
ENSG00000103253 E029 18.025700 0.0013639053 6.587971e-01 7.678029e-01 16 728504 728510 7 + 1.172 1.211 0.138
ENSG00000103253 E030 17.698823 0.0027865979 5.881666e-01 7.113874e-01 16 728511 728513 3 + 1.156 1.206 0.179
ENSG00000103253 E031 48.088643 0.0005050964 2.369030e-01 3.705890e-01 16 728514 728571 58 + 1.556 1.624 0.231
ENSG00000103253 E032 62.504359 0.0003891804 7.986511e-01 8.715205e-01 16 728572 728604 33 + 1.712 1.722 0.035
ENSG00000103253 E033 8.488114 0.0461009419 1.390727e-01 2.467979e-01 16 728605 728793 189 + 1.062 0.850 -0.793
ENSG00000103253 E034 102.093743 0.0003252611 7.037903e-01 8.024408e-01 16 728794 728895 102 + 1.920 1.932 0.042
ENSG00000103253 E035 25.458289 0.0088219628 3.966691e-03 1.304966e-02 16 728896 729008 113 + 1.514 1.271 -0.839
ENSG00000103253 E036 103.883702 0.0007120904 9.815282e-01 9.925197e-01 16 729009 729088 80 + 1.942 1.939 -0.011
ENSG00000103253 E037 50.013270 0.0004498066 6.075054e-02 1.278864e-01 16 729089 729287 199 + 1.702 1.597 -0.357
ENSG00000103253 E038 157.297074 0.0029230757 7.456171e-02 1.508360e-01 16 729288 729746 459 + 2.064 2.131 0.225
ENSG00000103253 E039 3.399885 0.0045712440 6.593301e-02 1.365877e-01 16 732658 733044 387 + 0.778 0.491 -1.249
ENSG00000103253 E040 1.119827 0.0393815627 9.141654e-02 1.773446e-01 16 733045 733054 10 + 0.508 0.196 -1.959
ENSG00000103253 E041 2.591508 0.0064650514 8.557309e-02 1.683252e-01 16 733055 733159 105 + 0.689 0.403 -1.346
ENSG00000103253 E042 0.742699 0.0184932739 1.000000e+00 1.000000e+00 16 733466 733468 3 + 0.192 0.197 0.047
ENSG00000103253 E043 26.917714 0.0007481990 1.860756e-02 4.847051e-02 16 733469 735525 2057 + 1.491 1.319 -0.594