ENSG00000103249

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262318 ENSG00000103249 HEK293_OSMI2_2hA HEK293_TMG_2hB CLCN7 protein_coding protein_coding 48.00518 48.00052 41.96366 3.520809 1.192071 -0.1938663 8.033685 10.79467 6.279814 1.2987866 1.0709365 -0.7805665 0.16817083 0.22350000 0.14880000 -0.074700000 1.290211e-01 5.797512e-70 FALSE TRUE
ENST00000382745 ENSG00000103249 HEK293_OSMI2_2hA HEK293_TMG_2hB CLCN7 protein_coding protein_coding 48.00518 48.00052 41.96366 3.520809 1.192071 -0.1938663 18.846116 26.48260 10.359253 1.3447214 0.3022646 -1.3532775 0.38726667 0.55366667 0.24760000 -0.306066667 1.190176e-20 5.797512e-70 FALSE TRUE
ENST00000565092 ENSG00000103249 HEK293_OSMI2_2hA HEK293_TMG_2hB CLCN7 protein_coding retained_intron 48.00518 48.00052 41.96366 3.520809 1.192071 -0.1938663 2.540135 2.60826 2.139699 0.6196635 0.3182879 -0.2844736 0.05205417 0.05503333 0.05110000 -0.003933333 9.920702e-01 5.797512e-70 FALSE TRUE
ENST00000567139 ENSG00000103249 HEK293_OSMI2_2hA HEK293_TMG_2hB CLCN7 protein_coding retained_intron 48.00518 48.00052 41.96366 3.520809 1.192071 -0.1938663 1.152349 0.00000 3.030704 0.0000000 0.7128630 8.2482615 0.02486667 0.00000000 0.07180000 0.071800000 1.816074e-15 5.797512e-70 FALSE FALSE
ENST00000567836 ENSG00000103249 HEK293_OSMI2_2hA HEK293_TMG_2hB CLCN7 protein_coding retained_intron 48.00518 48.00052 41.96366 3.520809 1.192071 -0.1938663 3.698921 4.76925 2.493191 0.8012589 0.2256740 -0.9330154 0.07417083 0.09896667 0.05963333 -0.039333333 1.135242e-01 5.797512e-70 FALSE FALSE
MSTRG.11770.6 ENSG00000103249 HEK293_OSMI2_2hA HEK293_TMG_2hB CLCN7 protein_coding   48.00518 48.00052 41.96366 3.520809 1.192071 -0.1938663 6.453283 0.00000 10.257034 0.0000000 0.5867931 10.0038038 0.14330833 0.00000000 0.24460000 0.244600000 5.797512e-70 5.797512e-70 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000103249 E001 0.6589510 0.0219909122 5.643470e-01 6.916502e-01 16 1444934 1444934 1 - 0.145 0.255 1.004
ENSG00000103249 E002 1.1038305 0.1020241378 2.017904e-01 3.284215e-01 16 1444935 1444935 1 - 0.145 0.410 1.980
ENSG00000103249 E003 43.3656719 0.0372358390 1.361547e-01 2.426994e-01 16 1444936 1445343 408 - 1.489 1.692 0.693
ENSG00000103249 E004 1478.0634936 0.0028278805 3.751187e-18 1.924319e-16 16 1445344 1446642 1299 - 2.983 3.222 0.795
ENSG00000103249 E005 275.7016095 0.0025509540 2.511716e-05 1.522143e-04 16 1446643 1446717 75 - 2.310 2.475 0.548
ENSG00000103249 E006 299.4005449 0.0007528757 9.368178e-05 4.922952e-04 16 1447006 1447083 78 - 2.375 2.496 0.403
ENSG00000103249 E007 185.5978922 0.0003208683 8.958124e-03 2.617471e-02 16 1447084 1447086 3 - 2.185 2.280 0.320
ENSG00000103249 E008 2.1380470 0.4108540693 9.727528e-01 9.870160e-01 16 1447388 1447391 4 - 0.477 0.492 0.072
ENSG00000103249 E009 423.6009156 0.0001292091 9.064591e-04 3.636413e-03 16 1447392 1447568 177 - 2.549 2.632 0.276
ENSG00000103249 E010 262.0044300 0.0001905977 7.973120e-01 8.706688e-01 16 1447655 1447714 60 - 2.377 2.405 0.092
ENSG00000103249 E011 18.9979475 0.0009414456 3.491706e-04 1.576899e-03 16 1448292 1448354 63 - 1.429 1.172 -0.901
ENSG00000103249 E012 382.2126535 0.0001696079 6.498998e-01 7.610315e-01 16 1448355 1448484 130 - 2.552 2.565 0.044
ENSG00000103249 E013 16.1855906 0.0220988944 3.115073e-01 4.539581e-01 16 1448485 1448608 124 - 1.268 1.175 -0.327
ENSG00000103249 E014 305.0392405 0.0001861728 2.294943e-02 5.764118e-02 16 1448681 1448766 86 - 2.480 2.453 -0.089
ENSG00000103249 E015 367.0748460 0.0025240498 6.408259e-01 7.539996e-01 16 1448966 1449093 128 - 2.536 2.549 0.042
ENSG00000103249 E016 17.0357572 0.0011337950 1.196529e-05 7.808162e-05 16 1449094 1449275 182 - 1.423 1.086 -1.187
ENSG00000103249 E017 231.3950451 0.0002790934 7.792071e-01 8.580174e-01 16 1449276 1449327 52 - 2.323 2.352 0.096
ENSG00000103249 E018 111.3631181 0.0073212420 5.198989e-08 5.586608e-07 16 1449328 1450496 1169 - 2.178 1.913 -0.888
ENSG00000103249 E019 347.1056971 0.0001540578 2.549394e-02 6.290122e-02 16 1450497 1450666 170 - 2.473 2.540 0.224
ENSG00000103249 E020 229.4972394 0.0058821244 7.774703e-01 8.567921e-01 16 1451623 1451703 81 - 2.316 2.354 0.125
ENSG00000103249 E021 114.9896000 0.0057230095 9.978588e-01 1.000000e+00 16 1451704 1451716 13 - 2.026 2.049 0.079
ENSG00000103249 E022 26.0153929 0.0188395079 2.734575e-09 3.743186e-08 16 1451717 1451969 253 - 1.677 1.127 -1.910
ENSG00000103249 E023 235.2476509 0.0011177185 4.902628e-01 6.276105e-01 16 1452755 1452893 139 - 2.322 2.364 0.142
ENSG00000103249 E024 176.6821096 0.0051454326 5.592126e-01 6.873538e-01 16 1453834 1453894 61 - 2.229 2.228 -0.002
ENSG00000103249 E025 225.5893626 0.0007891615 4.099727e-02 9.287584e-02 16 1454411 1454465 55 - 2.352 2.319 -0.111
ENSG00000103249 E026 320.0946500 0.0007988140 4.811830e-01 6.194318e-01 16 1455134 1455250 117 - 2.479 2.485 0.021
ENSG00000103249 E027 3.8916233 0.0557771105 7.633063e-05 4.106611e-04 16 1455492 1455730 239 - 0.988 0.314 -3.043
ENSG00000103249 E028 231.7251365 0.0001997797 4.302037e-01 5.728400e-01 16 1455731 1455795 65 - 2.315 2.357 0.138
ENSG00000103249 E029 238.4429489 0.0036536792 8.827575e-01 9.289650e-01 16 1456113 1456206 94 - 2.335 2.368 0.108
ENSG00000103249 E030 215.5925048 0.0007266865 6.281760e-01 7.437716e-01 16 1457254 1457328 75 - 2.305 2.314 0.031
ENSG00000103249 E031 133.8430759 0.0003065209 1.892985e-01 3.128449e-01 16 1457329 1457337 9 - 2.119 2.098 -0.069
ENSG00000103249 E032 5.4231205 0.0030863956 1.003043e-06 8.319041e-06 16 1457338 1457693 356 - 1.084 0.457 -2.579
ENSG00000103249 E033 217.2536433 0.0002503984 1.681015e-05 1.060625e-04 16 1457694 1457756 63 - 2.370 2.284 -0.288
ENSG00000103249 E034 221.8176266 0.0002231890 5.756851e-06 4.041722e-05 16 1459107 1459187 81 - 2.380 2.290 -0.302
ENSG00000103249 E035 15.4377596 0.0011227760 1.457243e-10 2.502904e-09 16 1459188 1459412 225 - 1.460 0.927 -1.901
ENSG00000103249 E036 261.7665492 0.0001886788 2.556241e-10 4.201199e-09 16 1460418 1460527 110 - 2.471 2.350 -0.404
ENSG00000103249 E037 3.9418555 0.0042775308 6.973137e-03 2.115326e-02 16 1460769 1460769 1 - 0.867 0.496 -1.578
ENSG00000103249 E038 7.4356106 0.0119088042 3.434809e-05 2.012860e-04 16 1460770 1460815 46 - 1.150 0.651 -1.916
ENSG00000103249 E039 175.7345307 0.0024642420 1.640095e-03 6.089510e-03 16 1460816 1460838 23 - 2.281 2.191 -0.301
ENSG00000103249 E040 280.7760120 0.0004054961 1.829345e-13 5.016123e-12 16 1460839 1460948 110 - 2.517 2.370 -0.489
ENSG00000103249 E041 217.9039736 0.0037950489 7.574305e-06 5.172031e-05 16 1461405 1461470 66 - 2.409 2.263 -0.488
ENSG00000103249 E042 195.2154057 0.0058031684 3.854105e-04 1.718361e-03 16 1461603 1461674 72 - 2.353 2.220 -0.443
ENSG00000103249 E043 136.1728251 0.0041591516 2.960432e-05 1.764252e-04 16 1465267 1465338 72 - 2.211 2.053 -0.529
ENSG00000103249 E044 0.0000000       16 1466599 1466788 190 -      
ENSG00000103249 E045 0.0000000       16 1471624 1471956 333 -      
ENSG00000103249 E046 0.2934659 0.0290785164 7.415052e-01   16 1474153 1474365 213 - 0.145 0.102 -0.582
ENSG00000103249 E047 111.6109592 0.0241976210 1.956864e-03 7.092173e-03 16 1474834 1475084 251 - 2.158 1.939 -0.733