ENSG00000103248

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000360900 ENSG00000103248 HEK293_OSMI2_2hA HEK293_TMG_2hB MTHFSD protein_coding protein_coding 17.26847 19.33908 14.6066 1.203498 0.7299733 -0.4046575 3.6934874 5.5833452 2.5136527 0.43912623 0.2756960 -1.14819800 0.20920417 0.28893333 0.17110000 -0.11783333 0.001004151 0.0001250055 FALSE TRUE
ENST00000562096 ENSG00000103248 HEK293_OSMI2_2hA HEK293_TMG_2hB MTHFSD protein_coding retained_intron 17.26847 19.33908 14.6066 1.203498 0.7299733 -0.4046575 2.4122863 2.0681078 2.2062206 0.01299889 0.4847215 0.09283101 0.13828333 0.10783333 0.14866667 0.04083333 0.446038074 0.0001250055 FALSE FALSE
ENST00000562940 ENSG00000103248 HEK293_OSMI2_2hA HEK293_TMG_2hB MTHFSD protein_coding protein_coding 17.26847 19.33908 14.6066 1.203498 0.7299733 -0.4046575 0.9781380 0.5695674 0.8666406 0.13781118 0.1778937 0.59700914 0.05860417 0.02880000 0.05876667 0.02996667 0.145147801 0.0001250055 FALSE TRUE
ENST00000568037 ENSG00000103248 HEK293_OSMI2_2hA HEK293_TMG_2hB MTHFSD protein_coding protein_coding 17.26847 19.33908 14.6066 1.203498 0.7299733 -0.4046575 1.7398139 2.3077598 0.6956590 0.32651765 0.4399615 -1.71568801 0.09987500 0.11843333 0.04726667 -0.07116667 0.437370038 0.0001250055 FALSE FALSE
ENST00000625049 ENSG00000103248 HEK293_OSMI2_2hA HEK293_TMG_2hB MTHFSD protein_coding TEC 17.26847 19.33908 14.6066 1.203498 0.7299733 -0.4046575 1.2409641 1.5766921 0.8436597 0.11799899 0.1050876 -0.89428930 0.06991250 0.08246667 0.05863333 -0.02383333 0.432557251 0.0001250055 FALSE TRUE
MSTRG.13188.10 ENSG00000103248 HEK293_OSMI2_2hA HEK293_TMG_2hB MTHFSD protein_coding   17.26847 19.33908 14.6066 1.203498 0.7299733 -0.4046575 0.8740154 0.8577329 0.9107100 0.40703450 0.3250903 0.08549584 0.05063333 0.04360000 0.06213333 0.01853333 0.816823308 0.0001250055 TRUE TRUE
MSTRG.13188.11 ENSG00000103248 HEK293_OSMI2_2hA HEK293_TMG_2hB MTHFSD protein_coding   17.26847 19.33908 14.6066 1.203498 0.7299733 -0.4046575 1.3680837 1.1124388 1.2744381 0.20263116 0.4341498 0.19450055 0.08025000 0.05700000 0.08776667 0.03076667 0.700073405 0.0001250055 FALSE TRUE
MSTRG.13188.14 ENSG00000103248 HEK293_OSMI2_2hA HEK293_TMG_2hB MTHFSD protein_coding   17.26847 19.33908 14.6066 1.203498 0.7299733 -0.4046575 0.9785075 1.4852007 0.5869777 0.20708157 0.3102085 -1.32459024 0.05769167 0.07873333 0.04200000 -0.03673333 0.653509725 0.0001250055 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000103248 E001 3.5794142 0.0044825340 2.115861e-01 3.403642e-01 16 86530178 86530185 8 - 0.755 0.572 -0.778
ENSG00000103248 E002 95.3622742 0.0003019065 6.959606e-08 7.286413e-07 16 86530186 86530832 647 - 1.804 2.019 0.723
ENSG00000103248 E003 236.2240956 0.0008496214 8.812264e-10 1.320042e-08 16 86530833 86531971 1139 - 2.231 2.395 0.547
ENSG00000103248 E004 72.0493332 0.0003631654 9.914931e-02 1.891827e-01 16 86531972 86532335 364 - 1.786 1.859 0.248
ENSG00000103248 E005 32.2661622 0.0008263587 5.270895e-01 6.602411e-01 16 86532336 86532361 26 - 1.464 1.506 0.144
ENSG00000103248 E006 59.9613228 0.0143544917 6.426426e-01 7.553931e-01 16 86532362 86532481 120 - 1.741 1.764 0.077
ENSG00000103248 E007 53.2111898 0.0049600303 2.637416e-01 4.016487e-01 16 86532482 86533800 1319 - 1.660 1.730 0.238
ENSG00000103248 E008 16.7830741 0.0014236845 6.699012e-01 7.765357e-01 16 86535208 86535208 1 - 1.202 1.240 0.137
ENSG00000103248 E009 20.2578997 0.0085311612 8.827636e-01 9.289650e-01 16 86535209 86535266 58 - 1.296 1.312 0.059
ENSG00000103248 E010 14.1782044 0.0206150481 8.015688e-01 8.735470e-01 16 86535267 86535267 1 - 1.178 1.155 -0.084
ENSG00000103248 E011 19.7697584 0.0275705820 6.669538e-01 7.743178e-01 16 86535268 86535299 32 - 1.323 1.289 -0.118
ENSG00000103248 E012 40.3532390 0.0139878396 7.291580e-01 8.213873e-01 16 86535300 86535515 216 - 1.609 1.590 -0.064
ENSG00000103248 E013 13.7960894 0.0013312123 3.744273e-03 1.242257e-02 16 86540720 86541198 479 - 1.305 1.049 -0.912
ENSG00000103248 E014 2.4711414 0.0078703213 2.844781e-01 4.247196e-01 16 86541535 86541696 162 - 0.644 0.475 -0.780
ENSG00000103248 E015 59.5471729 0.0035766445 8.258812e-01 8.903977e-01 16 86541697 86541738 42 - 1.767 1.757 -0.035
ENSG00000103248 E016 71.1152200 0.0006007812 5.110310e-01 6.461451e-01 16 86541739 86541822 84 - 1.853 1.826 -0.090
ENSG00000103248 E017 38.8608755 0.0005766812 7.156832e-01 8.115533e-01 16 86542101 86542109 9 - 1.585 1.566 -0.067
ENSG00000103248 E018 37.2442117 0.0006118625 4.101377e-01 5.537534e-01 16 86542110 86542111 2 - 1.585 1.539 -0.160
ENSG00000103248 E019 37.6063873 0.0005950712 5.915443e-01 7.142879e-01 16 86542112 86542116 5 - 1.581 1.551 -0.101
ENSG00000103248 E020 81.0690688 0.0003767761 1.609549e-01 2.763648e-01 16 86542117 86542213 97 - 1.922 1.868 -0.183
ENSG00000103248 E021 21.2262059 0.0009158157 2.389547e-03 8.428615e-03 16 86542214 86542545 332 - 1.458 1.234 -0.777
ENSG00000103248 E022 51.2008119 0.0017881290 1.465125e-01 2.569439e-01 16 86546559 86546604 46 - 1.738 1.663 -0.254
ENSG00000103248 E023 49.3632139 0.0127797600 2.699528e-01 4.085182e-01 16 86546605 86546649 45 - 1.724 1.643 -0.278
ENSG00000103248 E024 0.5921286 0.3091479104 7.792245e-01 8.580230e-01 16 86547146 86547183 38 - 0.154 0.234 0.748
ENSG00000103248 E025 3.6061905 0.0050618993 1.652310e-01 2.819818e-01 16 86547184 86547207 24 - 0.496 0.718 0.986
ENSG00000103248 E026 17.3477711 0.0013069420 8.298385e-01 8.931815e-01 16 86547208 86547323 116 - 1.256 1.240 -0.056
ENSG00000103248 E027 33.6055183 0.0023036677 1.839426e-02 4.799782e-02 16 86547324 86547568 245 - 1.403 1.565 0.557
ENSG00000103248 E028 10.7092657 0.0017816573 3.624964e-01 5.069117e-01 16 86548035 86548066 32 - 0.979 1.077 0.360
ENSG00000103248 E029 13.7320363 0.0013029295 3.847261e-01 5.288668e-01 16 86548067 86548111 45 - 1.082 1.167 0.304
ENSG00000103248 E030 54.1460637 0.0053587759 8.661879e-01 9.178232e-01 16 86548464 86548487 24 - 1.706 1.715 0.029
ENSG00000103248 E031 81.4410990 0.0003950939 1.299292e-01 2.340645e-01 16 86548488 86548577 90 - 1.927 1.868 -0.197
ENSG00000103248 E032 0.1472490 0.0423125935 2.916383e-01   16 86551973 86552032 60 - 0.154 0.000 -9.129
ENSG00000103248 E033 19.2392978 0.0012425654 1.442594e-02 3.919108e-02 16 86552033 86552037 5 - 1.403 1.215 -0.657
ENSG00000103248 E034 40.7014563 0.0006089807 6.893172e-04 2.860095e-03 16 86552038 86552146 109 - 1.710 1.526 -0.627
ENSG00000103248 E035 46.1918292 0.0065690563 7.613913e-01 8.452829e-01 16 86552179 86552292 114 - 1.631 1.653 0.075
ENSG00000103248 E036 0.4407149 0.0212811049 3.002591e-01 4.419244e-01 16 86553260 86553721 462 - 0.268 0.096 -1.779
ENSG00000103248 E037 66.2205964 0.0004473503 5.454482e-03 1.716704e-02 16 86554645 86554748 104 - 1.878 1.759 -0.402
ENSG00000103248 E038 21.5644949 0.0009479164 2.343151e-02 5.864721e-02 16 86554749 86554751 3 - 1.431 1.265 -0.579
ENSG00000103248 E039 4.3281693 0.3571029263 2.083710e-01 3.365217e-01 16 86554752 86555021 270 - 0.843 0.639 -0.828
ENSG00000103248 E040 2.6068364 0.0056975697 2.876347e-01 4.281468e-01 16 86555042 86555168 127 - 0.644 0.475 -0.777
ENSG00000103248 E041 31.7354848 0.0010190914 7.756800e-03 2.316638e-02 16 86555169 86555172 4 - 1.590 1.425 -0.566
ENSG00000103248 E042 31.7419282 0.0067022559 2.195846e-02 5.558835e-02 16 86555173 86555235 63 - 1.590 1.426 -0.564