ENSG00000103194

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000219473 ENSG00000103194 HEK293_OSMI2_2hA HEK293_TMG_2hB USP10 protein_coding protein_coding 75.38025 83.75664 85.4602 1.703261 2.424683 0.02904572 51.751081 41.69714 67.666334 1.470504 0.8828859 0.6983571 0.68728750 0.4988667 0.79263333 0.2937667 2.336416e-10 1.44039e-43 FALSE TRUE
ENST00000563048 ENSG00000103194 HEK293_OSMI2_2hA HEK293_TMG_2hB USP10 protein_coding nonsense_mediated_decay 75.38025 83.75664 85.4602 1.703261 2.424683 0.02904572 5.912193 10.87779 1.957816 1.699070 1.2726066 -2.4680441 0.08376667 0.1294667 0.02216667 -0.1073000 2.505552e-01 1.44039e-43 FALSE TRUE
ENST00000569925 ENSG00000103194 HEK293_OSMI2_2hA HEK293_TMG_2hB USP10 protein_coding protein_coding 75.38025 83.75664 85.4602 1.703261 2.424683 0.02904572 6.335239 21.95638 0.000000 3.277528 0.0000000 -11.1010815 0.07955000 0.2612333 0.00000000 -0.2612333 1.440390e-43 1.44039e-43 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000103194 E001 3.0730254 0.0053763817 1.496320e-01 2.611743e-01 16 84699986 84699989 4 + 0.703 0.504 -0.884
ENSG00000103194 E002 5.5581741 0.0098542424 4.536161e-02 1.008582e-01 16 84699990 84699994 5 + 0.916 0.669 -0.981
ENSG00000103194 E003 7.4737927 0.0109580314 8.945084e-02 1.742934e-01 16 84699995 84699999 5 + 0.999 0.814 -0.703
ENSG00000103194 E004 7.7682908 0.0033416825 4.260448e-02 9.582442e-02 16 84700000 84700001 2 + 1.023 0.813 -0.796
ENSG00000103194 E005 10.2957196 0.0019566426 8.130007e-02 1.615860e-01 16 84700002 84700003 2 + 1.109 0.957 -0.557
ENSG00000103194 E006 11.3922650 0.0015803157 8.760625e-02 1.715054e-01 16 84700004 84700007 4 + 1.147 1.007 -0.507
ENSG00000103194 E007 84.5571948 0.0031605079 5.465024e-12 1.186899e-10 16 84700008 84700028 21 + 2.050 1.747 -1.018
ENSG00000103194 E008 107.9629570 0.0045860087 1.522467e-05 9.702192e-05 16 84700029 84700039 11 + 2.110 1.936 -0.585
ENSG00000103194 E009 185.8177059 0.0034182958 4.616726e-06 3.312973e-05 16 84700040 84700111 72 + 2.334 2.189 -0.484
ENSG00000103194 E010 6.7536073 0.1033455017 1.490194e-01 2.603472e-01 16 84704740 84704860 121 + 0.986 0.760 -0.869
ENSG00000103194 E011 0.1472490 0.0431073733 5.658460e-01   16 84708872 84709322 451 + 0.110 0.000 -8.780
ENSG00000103194 E012 4.4159064 0.0039531587 7.411797e-01 8.304475e-01 16 84716195 84716258 64 + 0.750 0.732 -0.076
ENSG00000103194 E013 6.1851203 0.0033765578 7.648731e-01 8.478223e-01 16 84716259 84716518 260 + 0.867 0.859 -0.033
ENSG00000103194 E014 2.3822681 0.0156620149 1.818899e-01 3.035903e-01 16 84732468 84732589 122 + 0.388 0.633 1.190
ENSG00000103194 E015 6.7207691 0.0188225767 6.466653e-01 7.585038e-01 16 84733053 84733081 29 + 0.849 0.938 0.336
ENSG00000103194 E016 7.1646176 0.0323181612 6.034922e-01 7.242847e-01 16 84733082 84733105 24 + 0.867 0.971 0.394
ENSG00000103194 E017 0.1515154 0.0428965242 5.657995e-01   16 84733138 84733208 71 + 0.110 0.000 -8.781
ENSG00000103194 E018 0.2934659 0.0292255081 9.085738e-01   16 84733209 84733434 226 + 0.110 0.135 0.338
ENSG00000103194 E019 152.4024361 0.0055358713 7.925167e-05 4.243398e-04 16 84733435 84733447 13 + 2.250 2.100 -0.502
ENSG00000103194 E020 250.5562389 0.0026902362 2.473669e-08 2.831939e-07 16 84733448 84733503 56 + 2.468 2.313 -0.519
ENSG00000103194 E021 241.1370422 0.0008728943 2.429633e-11 4.755029e-10 16 84740309 84740369 61 + 2.449 2.302 -0.490
ENSG00000103194 E022 1130.5177657 0.0002193757 2.950057e-33 6.343694e-31 16 84744633 84745211 579 + 3.104 2.992 -0.373
ENSG00000103194 E023 838.7999519 0.0009533298 5.491168e-05 3.062335e-04 16 84745212 84745612 401 + 2.944 2.903 -0.137
ENSG00000103194 E024 352.4995976 0.0033968438 4.012668e-02 9.126904e-02 16 84745613 84745673 61 + 2.566 2.530 -0.118
ENSG00000103194 E025 404.9932006 0.0027293893 1.545630e-01 2.678342e-01 16 84758716 84758807 92 + 2.613 2.604 -0.031
ENSG00000103194 E026 4.3220035 0.0073805103 2.811943e-01 4.210746e-01 16 84759278 84759362 85 + 0.793 0.668 -0.511
ENSG00000103194 E027 463.6968331 0.0002105524 1.074799e-03 4.217516e-03 16 84759363 84759472 110 + 2.679 2.655 -0.080
ENSG00000103194 E028 2.2925205 0.2171760288 3.293616e-01 4.728625e-01 16 84759836 84759890 55 + 0.621 0.387 -1.144
ENSG00000103194 E029 403.6470733 0.0001497542 5.413252e-04 2.314744e-03 16 84759891 84759946 56 + 2.621 2.591 -0.100
ENSG00000103194 E030 2.2518587 0.0080627346 4.147900e-01 5.581849e-01 16 84759947 84760171 225 + 0.437 0.594 0.753
ENSG00000103194 E031 506.9488632 0.0001376396 5.879183e-03 1.829477e-02 16 84760172 84760275 104 + 2.711 2.699 -0.040
ENSG00000103194 E032 523.0078581 0.0001563591 1.906533e-01 3.145753e-01 16 84762989 84763088 100 + 2.711 2.723 0.038
ENSG00000103194 E033 487.6436671 0.0002323738 1.137882e-01 2.110436e-01 16 84764086 84764148 63 + 2.684 2.689 0.018
ENSG00000103194 E034 658.8825142 0.0007094073 7.634873e-01 8.467861e-01 16 84764149 84764263 115 + 2.804 2.831 0.092
ENSG00000103194 E035 393.3764917 0.0001713469 9.907328e-01 9.983689e-01 16 84768193 84768238 46 + 2.577 2.610 0.109
ENSG00000103194 E036 386.1551494 0.0001456443 5.512702e-01 6.807671e-01 16 84768239 84768264 26 + 2.575 2.597 0.072
ENSG00000103194 E037 337.4246547 0.0003410637 9.196716e-01 9.532536e-01 16 84768265 84768266 2 + 2.512 2.543 0.102
ENSG00000103194 E038 568.5579182 0.0001578167 1.128085e-01 2.096431e-01 16 84768267 84768358 92 + 2.725 2.782 0.191
ENSG00000103194 E039 509.7440380 0.0001762034 1.962870e-07 1.883598e-06 16 84772541 84772685 145 + 2.645 2.764 0.395
ENSG00000103194 E040 400.4250178 0.0001528567 5.636273e-12 1.221711e-10 16 84775160 84775225 66 + 2.518 2.677 0.529
ENSG00000103194 E041 2087.5695762 0.0027961450 6.222403e-24 6.139665e-22 16 84778895 84779922 1028 + 3.169 3.436 0.890