ENSG00000102924

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000219197 ENSG00000102924 HEK293_OSMI2_2hA HEK293_TMG_2hB CBLN1 protein_coding protein_coding 2.110406 1.975364 3.277972 0.08669556 0.2395736 0.7277942 1.5564798 1.975364 1.889283 0.08669556 0.1548820 -0.06394842 0.7809292 1 0.5794333 -0.4205667 1.268505e-13 1.268505e-13 FALSE TRUE
ENST00000536749 ENSG00000102924 HEK293_OSMI2_2hA HEK293_TMG_2hB CBLN1 protein_coding protein_coding 2.110406 1.975364 3.277972 0.08669556 0.2395736 0.7277942 0.4833832 0.000000 1.342896 0.00000000 0.2149582 7.07990676 0.1821167 0 0.4077667 0.4077667 3.076802e-12 1.268505e-13 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000102924 E001 71.7359121 0.0005783016 0.00376674 0.01248727 16 49277917 49279289 1373 - 1.812 1.906 0.316
ENSG00000102924 E002 10.6190187 0.0018752448 0.45622465 0.59667657 16 49279290 49279311 22 - 1.028 1.099 0.259
ENSG00000102924 E003 40.9066390 0.0008126478 0.55794879 0.68627679 16 49279312 49279601 290 - 1.634 1.603 -0.105
ENSG00000102924 E004 28.3340160 0.0011395263 0.01756594 0.04618885 16 49280923 49281042 120 - 1.540 1.394 -0.500
ENSG00000102924 E005 0.2966881 0.0274717676 0.22719011   16 49281043 49281201 159 - 0.210 0.000 -9.258
ENSG00000102924 E006 22.8138370 0.0061349248 0.18302708 0.30498664 16 49281202 49281473 272 - 1.429 1.330 -0.345
ENSG00000102924 E007 0.3289534 0.0282070477 0.94638329   16 49281474 49281526 53 - 0.118 0.127 0.126
ENSG00000102924 E008 12.7692085 0.0065441737 0.85716816 0.91177955 16 49281527 49281838 312 - 1.129 1.144 0.053