ENSG00000101856

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000217971 ENSG00000101856 HEK293_OSMI2_2hA HEK293_TMG_2hB PGRMC1 protein_coding protein_coding 156.6199 117.2936 212.4474 18.79268 3.188518 0.8569257 127.73487 77.21498 194.9357 5.964764 3.356419 1.335933 0.8079667 0.6745333 0.9179333 0.2434 1.197860e-04 1.023443e-31 FALSE TRUE
MSTRG.34776.5 ENSG00000101856 HEK293_OSMI2_2hA HEK293_TMG_2hB PGRMC1 protein_coding   156.6199 117.2936 212.4474 18.79268 3.188518 0.8569257 16.72265 33.94032 0.0000 13.479348 0.000000 -11.729210 0.1164208 0.2699000 0.0000000 -0.2699 1.023443e-31 1.023443e-31 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000101856 E001 9.7564455 0.0020884707 9.674602e-01 9.836542e-01 X 119236245 119236265 21 + 1.002 1.060 0.212
ENSG00000101856 E002 45.9187880 0.0126906718 4.419174e-01 5.835890e-01 X 119236266 119236284 19 + 1.661 1.652 -0.029
ENSG00000101856 E003 1390.2919288 0.0046251943 3.474962e-05 2.033173e-04 X 119236285 119236691 407 + 3.159 3.082 -0.256
ENSG00000101856 E004 1.4112044 0.0095643905 3.367351e-01 4.806076e-01 X 119237202 119237261 60 + 0.419 0.282 -0.832
ENSG00000101856 E005 484.2441441 0.0019432887 2.168158e-04 1.035916e-03 X 119240309 119240310 2 + 2.690 2.648 -0.138
ENSG00000101856 E006 1396.9248449 0.0001371657 7.843144e-22 6.117168e-20 X 119240311 119240464 154 + 3.149 3.115 -0.111
ENSG00000101856 E007 1311.6988611 0.0001183913 3.315188e-06 2.458708e-05 X 119243151 119243268 118 + 3.103 3.120 0.059
ENSG00000101856 E008 5058.6888740 0.0030973988 3.243150e-09 4.372057e-08 X 119243269 119244466 1198 + 3.633 3.773 0.466
ENSG00000101856 E009 0.5890081 0.0184091969 6.169494e-01 7.348869e-01 X 119250324 119250402 79 + 0.166 0.281 0.971