ENSG00000101654

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262173 ENSG00000101654 HEK293_OSMI2_2hA HEK293_TMG_2hB RNMT protein_coding protein_coding 7.750709 2.44433 10.69099 0.2838654 0.4515547 2.124343 0.4837055 0.89473047 0.000000 0.04370748 0.0000000 -6.499416 0.18097917 0.37266667 0.0000000 -0.3726667 1.846243e-29 1.846243e-29 FALSE TRUE
ENST00000383314 ENSG00000101654 HEK293_OSMI2_2hA HEK293_TMG_2hB RNMT protein_coding protein_coding 7.750709 2.44433 10.69099 0.2838654 0.4515547 2.124343 4.0833933 0.03722811 7.097084 0.03722811 0.2821168 7.233468 0.34183750 0.01296667 0.6653333 0.6523667 2.549895e-20 1.846243e-29 FALSE TRUE
ENST00000592764 ENSG00000101654 HEK293_OSMI2_2hA HEK293_TMG_2hB RNMT protein_coding protein_coding 7.750709 2.44433 10.69099 0.2838654 0.4515547 2.124343 1.8470983 0.89666940 2.448109 0.13984653 0.2886878 1.438901 0.24568333 0.38583333 0.2290000 -0.1568333 3.226359e-01 1.846243e-29 FALSE TRUE
ENST00000593007 ENSG00000101654 HEK293_OSMI2_2hA HEK293_TMG_2hB RNMT protein_coding protein_coding 7.750709 2.44433 10.69099 0.2838654 0.4515547 2.124343 0.3922022 0.56600543 0.000000 0.28450380 0.0000000 -5.848011 0.12475833 0.21013333 0.0000000 -0.2101333 2.762554e-02 1.846243e-29 FALSE FALSE
MSTRG.15535.2 ENSG00000101654 HEK293_OSMI2_2hA HEK293_TMG_2hB RNMT protein_coding   7.750709 2.44433 10.69099 0.2838654 0.4515547 2.124343 0.6034907 0.00000000 0.622949 0.00000000 0.3783683 5.984017 0.05011667 0.00000000 0.0573000 0.0573000 4.485278e-01 1.846243e-29 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000101654 E001 0.1472490 0.0441482260 1.000000e+00   18 13726660 13726666 7 + 0.057 0.000 -7.017
ENSG00000101654 E002 0.4375944 0.0277497986 9.113080e-01 9.476749e-01 18 13726667 13726672 6 + 0.152 0.000 -10.496
ENSG00000101654 E003 4.0038653 0.0040368305 1.738644e-01 2.932130e-01 18 13726673 13726703 31 + 0.653 0.400 -1.210
ENSG00000101654 E004 13.9051597 0.0012530853 6.376105e-06 4.432134e-05 18 13726704 13726729 26 + 1.142 0.400 -3.089
ENSG00000101654 E005 0.2966881 0.0270077753 1.000000e+00   18 13726769 13726865 97 + 0.107 0.000 -9.939
ENSG00000101654 E006 0.4847352 0.2306492077 4.456579e-01 5.872042e-01 18 13730438 13730626 189 + 0.107 0.245 1.434
ENSG00000101654 E007 24.4246175 0.0008054204 1.502691e-06 1.201538e-05 18 13730627 13730755 129 + 1.361 0.799 -2.053
ENSG00000101654 E008 0.0000000       18 13731474 13731475 2 +      
ENSG00000101654 E009 83.4407380 0.0003108358 2.624282e-10 4.305182e-09 18 13731476 13731934 459 + 1.863 1.505 -1.214
ENSG00000101654 E010 43.3260425 0.0005528839 3.321571e-01 4.757798e-01 18 13734464 13734599 136 + 1.544 1.495 -0.168
ENSG00000101654 E011 41.6414121 0.0077654523 7.278074e-02 1.479775e-01 18 13737010 13737135 126 + 1.542 1.403 -0.481
ENSG00000101654 E012 37.8852332 0.0005328954 5.859277e-02 1.242150e-01 18 13740167 13740279 113 + 1.502 1.375 -0.440
ENSG00000101654 E013 35.2960636 0.0007080803 1.516374e-02 4.085684e-02 18 13741510 13741619 110 + 1.481 1.299 -0.629
ENSG00000101654 E014 30.4139907 0.0012295915 1.094727e-02 3.103519e-02 18 13741620 13741691 72 + 1.421 1.208 -0.743
ENSG00000101654 E015 40.3124110 0.0005794983 1.922225e-03 6.984403e-03 18 13742488 13742652 165 + 1.542 1.315 -0.784
ENSG00000101654 E016 0.4772466 0.0205233792 5.057212e-02 1.101766e-01 18 13742653 13743074 422 + 0.057 0.400 3.433
ENSG00000101654 E017 24.4639524 0.0007832900 1.649116e-03 6.118207e-03 18 13746220 13746272 53 + 1.345 1.035 -1.104
ENSG00000101654 E018 29.0791285 0.0009561519 2.031030e-05 1.257550e-04 18 13746273 13746337 65 + 1.425 1.003 -1.496
ENSG00000101654 E019 0.1472490 0.0441482260 1.000000e+00   18 13752305 13752325 21 + 0.057 0.000 -8.789
ENSG00000101654 E020 38.5471159 0.0018792693 1.461729e-05 9.348777e-05 18 13752326 13752427 102 + 1.537 1.165 -1.296
ENSG00000101654 E021 24.8768585 0.0007810126 4.941627e-06 3.520652e-05 18 13754114 13754147 34 + 1.364 0.848 -1.869
ENSG00000101654 E022 46.2518997 0.0004838369 1.799290e-06 1.413871e-05 18 13759942 13760208 267 + 1.612 1.247 -1.261
ENSG00000101654 E023 185.5400601 0.0002188599 6.242515e-03 1.924959e-02 18 13760209 13762038 1830 + 2.173 2.102 -0.237
ENSG00000101654 E024 66.5475675 0.0004413576 1.510714e-01 2.631194e-01 18 13762039 13762300 262 + 1.729 1.667 -0.213
ENSG00000101654 E025 48.4022075 0.0005021901 3.559369e-01 5.003018e-01 18 13762301 13762437 137 + 1.568 1.645 0.262
ENSG00000101654 E026 311.1620065 0.0003080840 8.585435e-40 2.930603e-37 18 13762438 13764111 1674 + 2.317 2.605 0.961
ENSG00000101654 E027 85.8960950 0.0091780601 3.537067e-11 6.727104e-10 18 13764112 13764556 445 + 1.721 2.131 1.379