ENSG00000101558

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000340541 ENSG00000101558 HEK293_OSMI2_2hA HEK293_TMG_2hB VAPA protein_coding protein_coding 15.35035 8.748202 25.96904 0.7406979 0.9476185 1.568641 0.7786292 0.7070125 1.376891 0.1542696 0.05247474 0.9517846 0.0507750 0.07916667 0.05306667 -0.0261000 0.19554744 0.02208726 FALSE TRUE
ENST00000400000 ENSG00000101558 HEK293_OSMI2_2hA HEK293_TMG_2hB VAPA protein_coding protein_coding 15.35035 8.748202 25.96904 0.7406979 0.9476185 1.568641 8.6202997 6.7869146 12.650209 1.1248040 0.86705788 0.8973493 0.5990667 0.76586667 0.48733333 -0.2785333 0.02208726 0.02208726 FALSE TRUE
ENST00000577901 ENSG00000101558 HEK293_OSMI2_2hA HEK293_TMG_2hB VAPA protein_coding retained_intron 15.35035 8.748202 25.96904 0.7406979 0.9476185 1.568641 5.3625864 0.9468275 11.583737 0.4735466 1.05521994 3.5989429 0.2961208 0.11810000 0.44523333 0.3271333 0.30587303 0.02208726 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000101558 E001 28.4556887 0.0009724255 2.082363e-03 7.479364e-03 18 9914016 9914051 36 + 1.517 1.341 -0.607
ENSG00000101558 E002 38.8307857 0.0005479023 2.560588e-05 1.548590e-04 18 9914052 9914061 10 + 1.660 1.445 -0.735
ENSG00000101558 E003 38.6835366 0.0005439106 3.062684e-05 1.818290e-04 18 9914062 9914062 1 + 1.658 1.445 -0.728
ENSG00000101558 E004 249.7918358 0.0061212585 4.421460e-05 2.523901e-04 18 9914063 9914335 273 + 2.439 2.290 -0.499
ENSG00000101558 E005 1.8800450 0.0229642199 8.630780e-01 9.157009e-01 18 9914613 9915065 453 + 0.457 0.450 -0.033
ENSG00000101558 E006 0.1472490 0.0442303431 9.142059e-01   18 9915770 9915820 51 + 0.091 0.000 -10.211
ENSG00000101558 E007 0.3729606 0.0273502920 6.675813e-01 7.747709e-01 18 9924903 9924995 93 + 0.091 0.163 0.970
ENSG00000101558 E008 0.3299976 0.0274424043 8.528961e-02   18 9924996 9925074 79 + 0.000 0.281 12.870
ENSG00000101558 E009 0.8846375 0.8573640537 7.982210e-01 8.712089e-01 18 9931777 9931809 33 + 0.230 0.299 0.505
ENSG00000101558 E010 367.8764248 0.0013740973 2.425048e-06 1.852193e-05 18 9931810 9931962 153 + 2.586 2.504 -0.272
ENSG00000101558 E011 374.7825963 0.0001383497 2.809331e-02 6.819566e-02 18 9936110 9936213 104 + 2.566 2.567 0.003
ENSG00000101558 E012 0.1515154 0.0432428199 9.142251e-01   18 9936214 9936589 376 + 0.091 0.000 -10.212
ENSG00000101558 E013 1.6390069 0.0202815492 2.014878e-01 3.280304e-01 18 9936727 9936985 259 + 0.491 0.281 -1.207
ENSG00000101558 E014 361.1270852 0.0001836453 6.850823e-01 7.878806e-01 18 9936986 9937066 81 + 2.538 2.572 0.113
ENSG00000101558 E015 32.6495367 0.0006421251 6.331794e-01 7.479265e-01 18 9944924 9945058 135 + 1.517 1.528 0.038
ENSG00000101558 E016 7.1060394 0.0577130786 4.464025e-01 5.878895e-01 18 9947594 9950394 2801 + 0.834 0.968 0.509
ENSG00000101558 E017 521.3911886 0.0001788663 6.388479e-01 7.524032e-01 18 9950395 9950568 174 + 2.697 2.731 0.112
ENSG00000101558 E018 2.4526944 0.2322009974 9.979783e-01 1.000000e+00 18 9950569 9951353 785 + 0.523 0.561 0.176
ENSG00000101558 E019 1236.9128059 0.0046457941 8.493010e-06 5.735279e-05 18 9954053 9960021 5969 + 3.025 3.160 0.448