ENSG00000101544

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262198 ENSG00000101544 HEK293_OSMI2_2hA HEK293_TMG_2hB ADNP2 protein_coding protein_coding 10.67492 6.980156 16.24268 0.863604 0.3638649 1.217281 8.3639939 2.1382166 15.4332916 0.9913917 0.1009671 2.845769 0.69768750 0.2985667 0.95110000 0.6525333 0.0003718621 0.0003718621 FALSE TRUE
ENST00000561195 ENSG00000101544 HEK293_OSMI2_2hA HEK293_TMG_2hB ADNP2 protein_coding protein_coding 10.67492 6.980156 16.24268 0.863604 0.3638649 1.217281 0.3779342 0.8486415 0.0000000 0.8486415 0.0000000 -6.423984 0.04026667 0.1058667 0.00000000 -0.1058667 0.6993311784 0.0003718621   FALSE
MSTRG.15966.3 ENSG00000101544 HEK293_OSMI2_2hA HEK293_TMG_2hB ADNP2 protein_coding   10.67492 6.980156 16.24268 0.863604 0.3638649 1.217281 1.8252628 3.9932979 0.7207059 1.5494104 0.3666242 -2.453826 0.24930000 0.5955667 0.04343333 -0.5521333 0.0751714190 0.0003718621 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000101544 E001 1.3296214 0.0219834603 2.788390e-02 6.778460e-02 18 80109262 80109292 31 + 0.395 0.000 -12.819
ENSG00000101544 E002 5.7775256 0.0028063793 5.065081e-07 4.477042e-06 18 80109293 80109363 71 + 0.870 0.000 -14.934
ENSG00000101544 E003 30.4544038 0.0006515340 1.723864e-06 1.359960e-05 18 80109364 80109472 109 + 1.462 1.294 -0.585
ENSG00000101544 E004 0.7374030 0.0155994212 6.558129e-01 7.656423e-01 18 80109473 80109476 4 + 0.220 0.216 -0.034
ENSG00000101544 E005 0.2924217 0.0274168273 5.953071e-01   18 80109726 80110021 296 + 0.124 0.000 -10.648
ENSG00000101544 E006 41.8144337 0.0006261931 3.616700e-05 2.106539e-04 18 80117530 80117584 55 + 1.575 1.512 -0.213
ENSG00000101544 E007 42.5371912 0.0006236891 3.682590e-06 2.703253e-05 18 80117585 80117607 23 + 1.589 1.495 -0.324
ENSG00000101544 E008 39.6014490 0.0053639873 2.147869e-04 1.027492e-03 18 80117608 80117615 8 + 1.557 1.477 -0.276
ENSG00000101544 E009 48.8516711 0.0004779413 4.843295e-06 3.460224e-05 18 80117616 80117650 35 + 1.643 1.576 -0.228
ENSG00000101544 E010 71.8041088 0.0003791084 1.147550e-07 1.153070e-06 18 80133103 80133192 90 + 1.804 1.751 -0.181
ENSG00000101544 E011 0.5149242 0.4037322102 2.710646e-01 4.097614e-01 18 80133692 80133806 115 + 0.066 0.361 2.974
ENSG00000101544 E012 1365.2844972 0.0136299704 5.203971e-07 4.586967e-06 18 80135612 80140346 4735 + 2.993 3.240 0.820
ENSG00000101544 E013 1.9876142 0.3301098762 8.158575e-01 8.834423e-01 18 80147435 80147523 89 + 0.394 0.483 0.467