ENSG00000101452

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000252011 ENSG00000101452 HEK293_OSMI2_2hA HEK293_TMG_2hB DHX35 protein_coding protein_coding 8.367637 7.580794 7.63548 1.292751 0.2206406 0.01035629 4.2989219 3.5585999 3.157420 0.6117606 0.63512026 -0.1720501 0.50897917 0.47166667 0.4105000 -0.06116667 7.954806e-01 1.78716e-09 FALSE TRUE
ENST00000373323 ENSG00000101452 HEK293_OSMI2_2hA HEK293_TMG_2hB DHX35 protein_coding protein_coding 8.367637 7.580794 7.63548 1.292751 0.2206406 0.01035629 0.4235542 0.4323504 0.000000 0.2175763 0.00000000 -5.4671179 0.05382083 0.05946667 0.0000000 -0.05946667 7.536076e-04 1.78716e-09 FALSE TRUE
ENST00000449559 ENSG00000101452 HEK293_OSMI2_2hA HEK293_TMG_2hB DHX35 protein_coding protein_coding 8.367637 7.580794 7.63548 1.292751 0.2206406 0.01035629 1.0438130 2.2468449 0.000000 0.9668118 0.00000000 -7.8181635 0.11865000 0.28293333 0.0000000 -0.28293333 1.787160e-09 1.78716e-09 FALSE TRUE
ENST00000484417 ENSG00000101452 HEK293_OSMI2_2hA HEK293_TMG_2hB DHX35 protein_coding nonsense_mediated_decay 8.367637 7.580794 7.63548 1.292751 0.2206406 0.01035629 0.9223135 0.5199127 1.816443 0.0194574 0.07874698 1.7852103 0.11370000 0.07273333 0.2389000 0.16616667 1.211172e-06 1.78716e-09 FALSE TRUE
MSTRG.20707.2 ENSG00000101452 HEK293_OSMI2_2hA HEK293_TMG_2hB DHX35 protein_coding   8.367637 7.580794 7.63548 1.292751 0.2206406 0.01035629 1.1852069 0.6164328 2.064017 0.1463243 0.38902403 1.7271959 0.14643333 0.08326667 0.2714667 0.18820000 1.198998e-02 1.78716e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000101452 E001 0.2934659 0.0285665052 1.0000000000   20 38962299 38962341 43 + 0.118 0.127 0.122
ENSG00000101452 E002 0.2934659 0.0285665052 1.0000000000   20 38962342 38962343 2 + 0.118 0.127 0.122
ENSG00000101452 E003 0.2934659 0.0285665052 1.0000000000   20 38962344 38962349 6 + 0.118 0.127 0.122
ENSG00000101452 E004 1.1749974 0.0117834463 0.0318967748 0.0756535079 20 38962350 38962359 10 + 0.503 0.127 -2.684
ENSG00000101452 E005 18.4731135 0.0012870696 0.0062457658 0.0192575491 20 38962360 38962407 48 + 1.387 1.184 -0.712
ENSG00000101452 E006 33.1979149 0.0006594007 0.0176967939 0.0464806737 20 38969081 38969214 134 + 1.602 1.475 -0.433
ENSG00000101452 E007 37.5571424 0.0005779687 0.0170356278 0.0450130907 20 38972559 38972651 93 + 1.650 1.531 -0.406
ENSG00000101452 E008 32.1855283 0.0009176989 0.4140108273 0.5574294389 20 38983699 38983776 78 + 1.544 1.508 -0.121
ENSG00000101452 E009 4.7810197 0.0056681993 0.0009317136 0.0037254976 20 38988793 38988812 20 + 0.944 0.483 -1.935
ENSG00000101452 E010 35.5322547 0.0050400133 0.0179699771 0.0470709342 20 38988813 38988917 105 + 1.634 1.489 -0.495
ENSG00000101452 E011 22.7584432 0.0171736029 0.1753722999 0.2951491223 20 38991454 38991515 62 + 1.434 1.322 -0.391
ENSG00000101452 E012 35.1362622 0.0250837414 0.2066531646 0.3345423805 20 38992356 38992425 70 + 1.612 1.503 -0.371
ENSG00000101452 E013 39.0542225 0.0317786675 0.2075659956 0.3355613361 20 38994821 38994880 60 + 1.659 1.546 -0.386
ENSG00000101452 E014 56.1279970 0.0246914225 0.1832269894 0.3052499513 20 39001730 39001842 113 + 1.808 1.713 -0.322
ENSG00000101452 E015 47.4531490 0.0024658921 0.4560085521 0.5964845383 20 39002772 39002868 97 + 1.706 1.679 -0.093
ENSG00000101452 E016 62.3632943 0.0003764563 0.1065647358 0.2004862914 20 39003749 39003907 159 + 1.837 1.781 -0.187
ENSG00000101452 E017 66.7082191 0.0004408246 0.2026951005 0.3295933082 20 39006146 39006356 211 + 1.858 1.818 -0.132
ENSG00000101452 E018 55.9662930 0.0004512019 0.9934731619 1.0000000000 20 39010280 39010404 125 + 1.754 1.769 0.049
ENSG00000101452 E019 1.3402425 0.0364088842 0.1143262531 0.2118548200 20 39010512 39010579 68 + 0.503 0.224 -1.690
ENSG00000101452 E020 41.0750072 0.0005601632 0.6106811453 0.7299819707 20 39014880 39014934 55 + 1.634 1.621 -0.046
ENSG00000101452 E021 47.4356785 0.0005385137 0.3832294343 0.5274099147 20 39018804 39018899 96 + 1.704 1.674 -0.101
ENSG00000101452 E022 28.4921042 0.0007164255 0.8990917171 0.9396248400 20 39021841 39021872 32 + 1.463 1.485 0.075
ENSG00000101452 E023 38.8431635 0.0006103494 0.3618958117 0.5063072390 20 39021873 39021935 63 + 1.574 1.638 0.219
ENSG00000101452 E024 33.8539131 0.0030638908 0.0500502209 0.1092594654 20 39023690 39023767 78 + 1.472 1.609 0.467
ENSG00000101452 E025 52.4945146 0.0004958434 0.9793706946 0.9911527550 20 39025230 39025359 130 + 1.727 1.740 0.044
ENSG00000101452 E026 43.4184270 0.0032024693 0.3876535496 0.5317909762 20 39028418 39028499 82 + 1.622 1.687 0.222
ENSG00000101452 E027 6.4581301 0.0024984712 0.0000179615 0.0001125919 20 39029178 39030359 1182 + 1.076 0.529 -2.197
ENSG00000101452 E028 2.1432057 0.0244262706 0.7152046110 0.8111896596 20 39030360 39030703 344 + 0.458 0.529 0.346
ENSG00000101452 E029 50.2667639 0.0006182678 0.0023944366 0.0084431899 20 39030704 39030775 72 + 1.625 1.788 0.554
ENSG00000101452 E030 0.2934659 0.0285665052 1.0000000000   20 39034183 39034205 23 + 0.118 0.127 0.122
ENSG00000101452 E031 52.7359023 0.0004868611 0.0037895056 0.0125526378 20 39034206 39034317 112 + 1.653 1.805 0.515
ENSG00000101452 E032 260.0516191 0.0059057538 0.0001580390 0.0007830114 20 39038499 39039723 1225 + 2.337 2.484 0.487