ENSG00000101442

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000243903 ENSG00000101442 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTR5 protein_coding protein_coding 15.2677 19.2719 13.13296 2.344717 0.2452439 -0.5529571 2.377219 2.968848 2.538665 0.8765298 0.3736652 -0.2250129 0.1582250 0.1517333 0.1943667 0.04263333 0.713274028 0.005890753 FALSE TRUE
MSTRG.20699.1 ENSG00000101442 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTR5 protein_coding   15.2677 19.2719 13.13296 2.344717 0.2452439 -0.5529571 3.605751 3.488866 4.763966 0.5462967 1.0141722 0.4483007 0.2388667 0.1930667 0.3605333 0.16746667 0.247486212 0.005890753 TRUE TRUE
MSTRG.20699.4 ENSG00000101442 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTR5 protein_coding   15.2677 19.2719 13.13296 2.344717 0.2452439 -0.5529571 1.770744 1.907096 1.559408 0.8499478 0.3253074 -0.2887021 0.1194083 0.0900000 0.1197000 0.02970000 0.776104434 0.005890753 TRUE TRUE
MSTRG.20699.5 ENSG00000101442 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTR5 protein_coding   15.2677 19.2719 13.13296 2.344717 0.2452439 -0.5529571 7.507582 10.907088 4.270919 1.8235637 0.3183549 -1.3505957 0.4830583 0.5652000 0.3253667 -0.23983333 0.005890753 0.005890753 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000101442 E001 31.106480 0.0190042329 8.655205e-03 2.542056e-02 20 38748460 38748596 137 + 1.625 1.388 -0.815
ENSG00000101442 E002 25.086915 0.0633033811 1.597543e-01 2.747938e-01 20 38748597 38748633 37 + 1.515 1.312 -0.702
ENSG00000101442 E003 32.531208 0.0356095491 3.826900e-02 8.785365e-02 20 38748634 38748697 64 + 1.641 1.406 -0.807
ENSG00000101442 E004 78.026434 0.0018902965 5.453439e-06 3.849533e-05 20 38748698 38748853 156 + 1.996 1.802 -0.652
ENSG00000101442 E005 1.883254 0.0074391190 1.109664e-02 3.139759e-02 20 38750007 38750009 3 + 0.670 0.250 -2.239
ENSG00000101442 E006 148.327520 0.0002207966 1.961942e-04 9.476175e-04 20 38750010 38750239 230 + 2.220 2.122 -0.330
ENSG00000101442 E007 128.339392 0.0002669931 9.423767e-02 1.817548e-01 20 38752131 38752300 170 + 2.124 2.079 -0.150
ENSG00000101442 E008 129.420812 0.0003105005 4.453055e-01 5.868605e-01 20 38754957 38755174 218 + 2.077 2.109 0.105
ENSG00000101442 E009 143.859124 0.0020965117 4.742456e-01 6.130924e-01 20 38755857 38756039 183 + 2.124 2.156 0.109
ENSG00000101442 E010 178.083495 0.0002385041 7.730694e-01 8.535300e-01 20 38765402 38765518 117 + 2.229 2.244 0.052
ENSG00000101442 E011 106.898027 0.0003042130 7.172232e-01 8.126785e-01 20 38766238 38766256 19 + 2.020 2.015 -0.017
ENSG00000101442 E012 197.447231 0.0002187222 7.382613e-01 8.281844e-01 20 38766257 38766377 121 + 2.272 2.289 0.054
ENSG00000101442 E013 202.943134 0.0002108200 2.721484e-02 6.642835e-02 20 38767464 38767596 133 + 2.251 2.314 0.210
ENSG00000101442 E014 399.773607 0.0025022176 3.163612e-06 2.354681e-05 20 38771559 38772281 723 + 2.498 2.625 0.421
ENSG00000101442 E015 9.432383 0.0022708533 2.142081e-01 3.434912e-01 20 38772282 38772520 239 + 1.080 0.956 -0.457
ENSG00000101442 E016 3.659000 0.0042296606 6.682536e-04 2.782388e-03 20 38779706 38779852 147 + 0.900 0.408 -2.158