ENSG00000101417

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000217398 ENSG00000101417 HEK293_OSMI2_2hA HEK293_TMG_2hB PXMP4 protein_coding protein_coding 3.587612 5.839959 2.102669 0.480661 0.1324109 -1.46936 1.3827491 3.3295509 0.5884607 0.23121728 0.20223834 -2.4803258 0.3375000 0.5715333 0.2726333 -0.2989000 0.0191046 0.0191046 FALSE TRUE
ENST00000344022 ENSG00000101417 HEK293_OSMI2_2hA HEK293_TMG_2hB PXMP4 protein_coding protein_coding 3.587612 5.839959 2.102669 0.480661 0.1324109 -1.46936 0.9070467 0.8675371 0.6207960 0.28443140 0.06258772 -0.4762866 0.2779917 0.1421667 0.2974000 0.1552333 0.1925902 0.0191046   FALSE
ENST00000409299 ENSG00000101417 HEK293_OSMI2_2hA HEK293_TMG_2hB PXMP4 protein_coding protein_coding 3.587612 5.839959 2.102669 0.480661 0.1324109 -1.46936 1.2978161 1.6428712 0.8934127 0.03397365 0.07041492 -0.8715172 0.3845167 0.2862667 0.4300000 0.1437333 0.1958184 0.0191046 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000101417 E001 152.1502451 0.0099698298 1.122250e-01 0.2087942192 20 33702758 33707268 4511 - 2.167 2.101 -0.219
ENSG00000101417 E002 17.4529400 0.0013807974 8.125348e-05 0.0004339054 20 33707269 33707488 220 - 1.420 1.089 -1.166
ENSG00000101417 E003 77.2844691 0.0081642094 2.450186e-02 0.0608545630 20 33707489 33707969 481 - 1.734 1.848 0.385
ENSG00000101417 E004 43.2483391 0.0005943954 9.015150e-03 0.0263161695 20 33710555 33710753 199 - 1.461 1.609 0.505
ENSG00000101417 E005 0.2965864 0.1779536691 6.626323e-01   20 33710754 33710773 20 - 0.001 0.144 8.155
ENSG00000101417 E006 22.9458975 0.0010072822 9.755746e-01 0.9887477050 20 33714674 33714736 63 - 1.323 1.316 -0.024
ENSG00000101417 E007 22.8215246 0.0009171865 9.031741e-01 0.9423977642 20 33720095 33720319 225 - 1.312 1.312 0.001