ENSG00000101365

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000380843 ENSG00000101365 HEK293_OSMI2_2hA HEK293_TMG_2hB IDH3B protein_coding protein_coding 166.7382 254.3815 104.9544 17.2867 1.355847 -1.277151 100.74179 173.66713 53.80953 11.016931 1.626494 -1.6902059 0.5790500 0.6832667 0.5124667 -0.1708 6.041922e-14 6.041922e-14 FALSE TRUE
ENST00000474315 ENSG00000101365 HEK293_OSMI2_2hA HEK293_TMG_2hB IDH3B protein_coding protein_coding 166.7382 254.3815 104.9544 17.2867 1.355847 -1.277151 32.45224 49.89412 24.95750 5.751059 2.140363 -0.9991075 0.2003458 0.1948000 0.2382000 0.0434 4.306022e-01 6.041922e-14 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000101365 E001 604.59542 2.422170e-03 4.126839e-03 1.349730e-02 20 2658395 2658522 128 - 2.623 2.708 0.285
ENSG00000101365 E002 404.93323 1.876097e-03 5.904595e-06 4.135334e-05 20 2658523 2658541 19 - 2.405 2.545 0.466
ENSG00000101365 E003 773.89856 1.445728e-03 6.355413e-07 5.507085e-06 20 2658542 2658637 96 - 2.702 2.820 0.395
ENSG00000101365 E004 1083.18631 2.549739e-03 2.070220e-03 7.442350e-03 20 2658638 2658758 121 - 2.879 2.958 0.262
ENSG00000101365 E005 1258.23092 1.508409e-03 2.444070e-02 6.073291e-02 20 2658759 2658837 79 - 2.972 3.017 0.149
ENSG00000101365 E006 51.29633 2.740675e-02 8.033646e-06 5.453064e-05 20 2659015 2659282 268 - 1.924 1.495 -1.457
ENSG00000101365 E007 25.41381 2.108111e-02 6.811067e-05 3.710315e-04 20 2659283 2659312 30 - 1.610 1.213 -1.373
ENSG00000101365 E008 48.96259 1.607800e-02 1.971171e-04 9.514929e-04 20 2659313 2659524 212 - 1.830 1.521 -1.052
ENSG00000101365 E009 1105.83559 3.712443e-04 2.708845e-10 4.438144e-09 20 2659525 2659585 61 - 2.874 2.973 0.326
ENSG00000101365 E010 1125.80065 8.654356e-05 2.129172e-22 1.775375e-20 20 2659699 2659793 95 - 2.854 2.988 0.443
ENSG00000101365 E011 47.69869 5.136621e-03 1.944918e-19 1.167487e-17 20 2659794 2660029 236 - 1.968 1.414 -1.879
ENSG00000101365 E012 565.25982 1.111762e-04 2.225482e-14 6.954981e-13 20 2660030 2660031 2 - 2.544 2.692 0.493
ENSG00000101365 E013 848.30626 9.359357e-05 1.806630e-11 3.603881e-10 20 2660032 2660067 36 - 2.755 2.860 0.346
ENSG00000101365 E014 1222.01729 7.405657e-05 2.456020e-16 1.007984e-14 20 2660068 2660176 109 - 2.914 3.019 0.351
ENSG00000101365 E015 872.13092 1.108899e-03 9.060559e-01 9.442657e-01 20 2660263 2660295 33 - 2.851 2.851 -0.001
ENSG00000101365 E016 1044.48868 1.066985e-03 6.661426e-01 7.736429e-01 20 2660296 2660365 70 - 2.938 2.926 -0.041
ENSG00000101365 E017 38.29398 5.595853e-04 2.002872e-23 1.858169e-21 20 2660366 2660456 91 - 1.886 1.321 -1.931
ENSG00000101365 E018 1110.13913 4.090243e-04 8.495222e-06 5.736568e-05 20 2660457 2660504 48 - 3.011 2.941 -0.231
ENSG00000101365 E019 1417.07891 2.025781e-04 3.614745e-11 6.863124e-10 20 2660505 2660590 86 - 3.127 3.044 -0.276
ENSG00000101365 E020 37.76483 3.406807e-03 7.913073e-23 6.898794e-21 20 2660591 2660696 106 - 1.899 1.282 -2.112
ENSG00000101365 E021 1752.03529 1.794005e-04 7.719821e-16 2.959856e-14 20 2660697 2660829 133 - 3.225 3.134 -0.302
ENSG00000101365 E022 21.82835 3.753999e-02 1.390726e-05 8.938423e-05 20 2660830 2660908 79 - 1.616 1.096 -1.813
ENSG00000101365 E023 1109.41928 1.258734e-04 2.867757e-07 2.665267e-06 20 2660909 2660969 61 - 3.010 2.942 -0.226
ENSG00000101365 E024 1374.16683 4.375745e-04 2.840037e-07 2.641189e-06 20 2663446 2663566 121 - 3.108 3.030 -0.259
ENSG00000101365 E025 1110.11864 1.452090e-03 3.554679e-02 8.270370e-02 20 2663660 2663758 99 - 2.994 2.942 -0.174
ENSG00000101365 E026 715.97772 3.093248e-03 4.878355e-02 1.069998e-01 20 2663925 2664005 81 - 2.816 2.748 -0.226
ENSG00000101365 E027 492.11358 3.649206e-03 1.088421e-01 2.038330e-01 20 2664153 2664219 67 - 2.649 2.586 -0.210