ENSG00000101216

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000370069 ENSG00000101216 HEK293_OSMI2_2hA HEK293_TMG_2hB GMEB2 protein_coding protein_coding 12.43884 17.65169 10.08904 1.271438 0.4657361 -0.8064051 3.2014221 7.2817606 0.00000 0.26789365 0.0000000 -9.5101234 0.21323333 0.41596667 0.0000000 -0.4159667 2.075735e-40 2.075735e-40 FALSE TRUE
ENST00000370077 ENSG00000101216 HEK293_OSMI2_2hA HEK293_TMG_2hB GMEB2 protein_coding protein_coding 12.43884 17.65169 10.08904 1.271438 0.4657361 -0.8064051 7.9683221 9.1893150 8.43048 0.98902640 0.3516356 -0.1242013 0.67255417 0.51803333 0.8359667 0.3179333 4.571266e-15 2.075735e-40 FALSE TRUE
MSTRG.20992.8 ENSG00000101216 HEK293_OSMI2_2hA HEK293_TMG_2hB GMEB2 protein_coding   12.43884 17.65169 10.08904 1.271438 0.4657361 -0.8064051 0.5854666 0.3768189 1.03982 0.04325675 0.0621417 1.4404116 0.05755417 0.02193333 0.1039333 0.0820000 3.241675e-07 2.075735e-40 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000101216 E001 0.0000000       20 63587602 63587604 3 -      
ENSG00000101216 E002 1102.5682757 0.0017194431 4.850801e-14 1.442200e-12 20 63587605 63590257 2653 - 2.905 3.051 0.485
ENSG00000101216 E003 127.1525457 0.0002506817 8.438445e-01 9.027941e-01 20 63590258 63590490 233 - 2.040 2.083 0.143
ENSG00000101216 E004 53.9198862 0.0006017668 5.798578e-01 7.046920e-01 20 63590491 63590540 50 - 1.704 1.711 0.025
ENSG00000101216 E005 99.2027204 0.0002950093 2.161035e-01 3.457141e-01 20 63590541 63590729 189 - 1.971 1.961 -0.034
ENSG00000101216 E006 80.0152814 0.0005645329 8.436723e-01 9.026747e-01 20 63592022 63592144 123 - 1.848 1.876 0.093
ENSG00000101216 E007 6.8953400 0.0238390441 1.477266e-02 3.998053e-02 20 63592145 63592271 127 - 1.058 0.766 -1.111
ENSG00000101216 E008 92.4454442 0.0003546722 6.184335e-01 7.360614e-01 20 63592533 63592670 138 - 1.922 1.939 0.056
ENSG00000101216 E009 92.4598429 0.0004731567 6.123019e-03 1.893054e-02 20 63593011 63593082 72 - 1.979 1.910 -0.233
ENSG00000101216 E010 131.7254561 0.0003352061 3.059943e-04 1.403795e-03 20 63595610 63595767 158 - 2.137 2.058 -0.265
ENSG00000101216 E011 102.6946075 0.0022420836 1.242363e-02 3.455521e-02 20 63597757 63597860 104 - 2.023 1.957 -0.222
ENSG00000101216 E012 104.0874458 0.0003787819 9.522256e-03 2.759323e-02 20 63602965 63603087 123 - 2.023 1.965 -0.194
ENSG00000101216 E013 44.3527313 0.0006014074 6.397430e-02 1.333099e-01 20 63603088 63603092 5 - 1.661 1.594 -0.226
ENSG00000101216 E014 87.7362992 0.0018143749 2.624699e-04 1.226575e-03 20 63604743 63604840 98 - 1.987 1.869 -0.397
ENSG00000101216 E015 0.2214452 0.0370954809 9.625243e-01   20 63604841 63604902 62 - 0.000 0.095 8.208
ENSG00000101216 E016 81.4687702 0.0003334037 1.478359e-08 1.766005e-07 20 63619267 63619454 188 - 1.999 1.810 -0.633
ENSG00000101216 E017 4.8211126 0.0036391204 1.847198e-01 3.071158e-01 20 63619455 63619876 422 - 0.557 0.787 0.974
ENSG00000101216 E018 23.2553546 0.0230376556 7.000542e-06 4.821784e-05 20 63626956 63627112 157 - 1.593 1.169 -1.473
ENSG00000101216 E019 1.3243202 0.0145569775 3.625701e-01 5.069803e-01 20 63628104 63628260 157 - 0.437 0.296 -0.834
ENSG00000101216 E020 3.8268812 0.0042117516 8.211103e-01 8.872335e-01 20 63628261 63628805 545 - 0.651 0.645 -0.026