ENSG00000101193

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000266069 ENSG00000101193 HEK293_OSMI2_2hA HEK293_TMG_2hB GID8 protein_coding protein_coding 29.72208 31.86949 28.77588 2.552954 0.9179594 -0.1472675 18.242148 23.245494 15.927730 2.8027490 0.3839584 -0.5451253658 0.6120917 0.7244333 0.5542667 -0.17016667 0.00717519 0.00717519 FALSE TRUE
MSTRG.20978.2 ENSG00000101193 HEK293_OSMI2_2hA HEK293_TMG_2hB GID8 protein_coding   29.72208 31.86949 28.77588 2.552954 0.9179594 -0.1472675 3.264738 1.160393 4.965833 0.5857488 0.5958156 2.0879450011 0.1107583 0.0391000 0.1726000 0.13350000 0.33327893 0.00717519 FALSE FALSE
MSTRG.20978.4 ENSG00000101193 HEK293_OSMI2_2hA HEK293_TMG_2hB GID8 protein_coding   29.72208 31.86949 28.77588 2.552954 0.9179594 -0.1472675 6.830521 6.402349 6.404425 0.4843355 0.4067658 0.0004671537 0.2307875 0.2040000 0.2222333 0.01823333 0.81165786 0.00717519 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000101193 E001 0.3666179 0.0350705206 7.927592e-01 8.675003e-01 20 62938109 62938109 1 + 0.143 0.105 -0.514
ENSG00000101193 E002 1.2502363 0.0204555027 9.248272e-01 9.566561e-01 20 62938110 62938112 3 + 0.336 0.320 -0.098
ENSG00000101193 E003 1.4048722 0.0138603980 8.468319e-01 9.048237e-01 20 62938113 62938113 1 + 0.336 0.373 0.221
ENSG00000101193 E004 24.2712461 0.0149497992 7.290920e-01 8.213714e-01 20 62938114 62938146 33 + 1.407 1.374 -0.115
ENSG00000101193 E005 27.9067864 0.0115937498 5.890068e-01 7.121378e-01 20 62938147 62938152 6 + 1.474 1.429 -0.155
ENSG00000101193 E006 134.9327205 0.0051807152 5.769317e-05 3.200711e-04 20 62938153 62938253 101 + 2.226 2.040 -0.624
ENSG00000101193 E007 4.3106754 0.0461589824 1.688841e-01 2.867258e-01 20 62938597 62938599 3 + 0.835 0.604 -0.952
ENSG00000101193 E008 9.8829298 0.0020455636 1.463570e-03 5.518448e-03 20 62938600 62938723 124 + 1.199 0.877 -1.181
ENSG00000101193 E009 17.9943535 0.0011718260 3.278040e-05 1.932098e-04 20 62938724 62938824 101 + 1.439 1.113 -1.146
ENSG00000101193 E010 315.0197840 0.0018495043 7.828085e-08 8.114507e-07 20 62941491 62941620 130 + 2.573 2.425 -0.494
ENSG00000101193 E011 542.8189024 0.0001407558 1.041807e-13 2.953716e-12 20 62942987 62943183 197 + 2.788 2.681 -0.355
ENSG00000101193 E012 220.2561941 0.0001796055 6.658536e-05 3.636111e-04 20 62943495 62943500 6 + 2.388 2.298 -0.299
ENSG00000101193 E013 531.6361016 0.0001637363 6.310189e-03 1.942899e-02 20 62943501 62943692 192 + 2.738 2.701 -0.123
ENSG00000101193 E014 1575.0844049 0.0001568336 3.882504e-02 8.885881e-02 20 62944739 62946855 2117 + 3.172 3.194 0.073
ENSG00000101193 E015 509.7239039 0.0009140047 7.488025e-07 6.390593e-06 20 62946856 62947659 804 + 2.627 2.733 0.354
ENSG00000101193 E016 491.9625705 0.0008372770 1.886881e-36 5.113934e-34 20 62947660 62948475 816 + 2.492 2.762 0.902