ENSG00000101158

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000474543 ENSG00000101158 HEK293_OSMI2_2hA HEK293_TMG_2hB NELFCD protein_coding retained_intron 97.69017 115.8818 84.13933 10.36048 1.60942 -0.4617552 5.02671 3.214119 5.098964 0.2627076 0.4369906 0.6641255 0.05337083 0.02856667 0.06046667 0.03190000 4.994901e-03 8.60222e-33 TRUE TRUE
ENST00000497935 ENSG00000101158 HEK293_OSMI2_2hA HEK293_TMG_2hB NELFCD protein_coding processed_transcript 97.69017 115.8818 84.13933 10.36048 1.60942 -0.4617552 14.63141 11.029860 14.938913 3.7829951 0.9205503 0.4373187 0.15347500 0.09480000 0.17736667 0.08256667 2.539367e-01 8.60222e-33 FALSE FALSE
ENST00000652272 ENSG00000101158 HEK293_OSMI2_2hA HEK293_TMG_2hB NELFCD protein_coding protein_coding 97.69017 115.8818 84.13933 10.36048 1.60942 -0.4617552 62.31272 86.614626 44.436042 8.2569699 1.5192291 -0.9627223 0.62880417 0.74690000 0.52903333 -0.21786667 3.300085e-05 8.60222e-33 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000101158 E001 5.306999 3.179851e-03 5.191112e-02 1.125684e-01 20 58981208 58981251 44 + 0.922 0.669 -1.005
ENSG00000101158 E002 6.523725 2.577417e-03 1.592548e-02 4.256479e-02 20 58981252 58981255 4 + 1.022 0.732 -1.115
ENSG00000101158 E003 20.311032 7.002650e-03 6.866102e-05 3.736749e-04 20 58981256 58981274 19 + 1.496 1.160 -1.173
ENSG00000101158 E004 228.801703 3.224925e-03 3.206250e-03 1.087349e-02 20 58981275 58981369 95 + 2.403 2.294 -0.363
ENSG00000101158 E005 418.459910 1.986859e-03 1.140675e-01 2.114477e-01 20 58986093 58986208 116 + 2.624 2.580 -0.146
ENSG00000101158 E006 4.873688 1.147217e-02 2.214831e-03 7.889162e-03 20 58986209 58986753 545 + 0.985 0.567 -1.690
ENSG00000101158 E007 513.572829 1.373464e-03 4.947177e-01 6.316517e-01 20 58986754 58986863 110 + 2.694 2.677 -0.057
ENSG00000101158 E008 16.424324 4.874890e-03 5.488954e-19 3.119036e-17 20 58986864 58987421 558 + 1.587 0.770 -2.946
ENSG00000101158 E009 252.574186 4.147279e-03 1.523372e-01 2.648760e-01 20 58987708 58987709 2 + 2.413 2.355 -0.192
ENSG00000101158 E010 519.336626 5.375303e-04 1.058875e-02 3.018275e-02 20 58987710 58987817 108 + 2.721 2.673 -0.161
ENSG00000101158 E011 3.773998 1.144105e-02 2.030720e-06 1.577666e-05 20 58987818 58987969 152 + 1.004 0.296 -3.219
ENSG00000101158 E012 518.642989 1.448738e-04 5.243748e-03 1.659476e-02 20 58988914 58989018 105 + 2.720 2.676 -0.146
ENSG00000101158 E013 316.993824 2.665793e-04 5.154342e-04 2.217144e-03 20 58989019 58989021 3 + 2.526 2.454 -0.239
ENSG00000101158 E014 51.460433 4.441794e-04 1.418287e-63 1.599346e-60 20 58989022 58989487 466 + 2.077 1.202 -2.987
ENSG00000101158 E015 654.532080 1.224082e-04 9.277313e-03 2.697795e-02 20 58989488 58989640 153 + 2.815 2.779 -0.120
ENSG00000101158 E016 544.696881 1.192859e-04 4.064663e-01 5.501098e-01 20 58989858 58989988 131 + 2.700 2.716 0.054
ENSG00000101158 E017 33.497499 1.055365e-03 6.930838e-24 6.803246e-22 20 58989989 58990094 106 + 1.825 1.208 -2.120
ENSG00000101158 E018 28.973431 6.849284e-04 4.387999e-23 3.928607e-21 20 58990095 58990232 138 + 1.774 1.137 -2.203
ENSG00000101158 E019 72.182036 3.943245e-04 2.835818e-57 2.582871e-54 20 58990233 58990895 663 + 2.170 1.492 -2.288
ENSG00000101158 E020 18.884293 9.923942e-04 6.989776e-11 1.265958e-09 20 58990896 58990909 14 + 1.541 1.033 -1.789
ENSG00000101158 E021 646.659950 2.121301e-04 7.420915e-01 8.311020e-01 20 58990910 58991036 127 + 2.780 2.787 0.025
ENSG00000101158 E022 510.003654 1.297692e-04 1.535965e-02 4.130536e-02 20 58991037 58991075 39 + 2.708 2.670 -0.126
ENSG00000101158 E023 22.750151 2.852868e-03 3.640556e-23 3.285815e-21 20 58991076 58991311 236 + 1.706 0.934 -2.715
ENSG00000101158 E024 935.611818 1.691508e-04 1.371376e-03 5.213056e-03 20 58991312 58991446 135 + 2.971 2.931 -0.133
ENSG00000101158 E025 30.708549 1.969848e-03 2.794399e-32 5.497306e-30 20 58991447 58991880 434 + 1.842 1.042 -2.772
ENSG00000101158 E026 932.078540 1.077465e-04 2.089477e-01 3.372626e-01 20 58991881 58992020 140 + 2.952 2.938 -0.046
ENSG00000101158 E027 751.323516 9.675312e-05 8.655884e-08 8.894242e-07 20 58992998 58993112 115 + 2.795 2.873 0.260
ENSG00000101158 E028 14.796009 1.642937e-02 5.261638e-08 5.645682e-07 20 58993113 58993448 336 + 1.471 0.895 -2.061
ENSG00000101158 E029 618.816577 1.620623e-04 2.039940e-10 3.413058e-09 20 58993449 58993544 96 + 2.691 2.796 0.349
ENSG00000101158 E030 482.417282 3.144215e-04 3.616285e-04 1.625257e-03 20 58993624 58993675 52 + 2.610 2.680 0.233
ENSG00000101158 E031 577.125103 1.160106e-04 1.075374e-05 7.094030e-05 20 58993676 58993764 89 + 2.684 2.758 0.246
ENSG00000101158 E032 68.164014 2.182771e-03 1.201720e-32 2.453258e-30 20 58993765 58993922 158 + 2.110 1.538 -1.932
ENSG00000101158 E033 65.593611 6.642864e-03 2.553739e-18 1.338308e-16 20 58993923 58994109 187 + 2.072 1.554 -1.750
ENSG00000101158 E034 720.163551 2.983185e-04 2.511790e-06 1.912508e-05 20 58994110 58994191 82 + 2.778 2.855 0.256
ENSG00000101158 E035 626.080553 2.854339e-04 5.485604e-06 3.868922e-05 20 58994192 58994239 48 + 2.716 2.794 0.260
ENSG00000101158 E036 958.279036 4.159294e-04 3.914226e-17 1.766829e-15 20 58994642 58994824 183 + 2.862 2.993 0.435
ENSG00000101158 E037 641.863410 1.689243e-03 1.649060e-09 2.347707e-08 20 58994825 58994925 101 + 2.673 2.823 0.500
ENSG00000101158 E038 564.938146 7.934497e-04 1.029236e-09 1.523088e-08 20 58994926 58995133 208 + 2.634 2.763 0.430