ENSG00000101146

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000371242 ENSG00000101146 HEK293_OSMI2_2hA HEK293_TMG_2hB RAE1 protein_coding protein_coding 65.97402 73.96653 61.79879 7.224566 0.7559548 -0.2592557 4.667542 5.683796 4.827547 0.9264706 0.8639929 -0.2351151 0.07146667 0.07703333 0.07846667 0.001433333 1.000000e+00 1.75981e-23 FALSE TRUE
ENST00000395841 ENSG00000101146 HEK293_OSMI2_2hA HEK293_TMG_2hB RAE1 protein_coding protein_coding 65.97402 73.96653 61.79879 7.224566 0.7559548 -0.2592557 26.210605 42.611961 18.163679 6.1483151 0.9869316 -1.2297464 0.38629167 0.57050000 0.29423333 -0.276266667 5.837542e-08 1.75981e-23 FALSE TRUE
ENST00000429339 ENSG00000101146 HEK293_OSMI2_2hA HEK293_TMG_2hB RAE1 protein_coding protein_coding 65.97402 73.96653 61.79879 7.224566 0.7559548 -0.2592557 5.631994 0.000000 8.564715 0.0000000 2.1077713 9.7439449 0.08357083 0.00000000 0.13790000 0.137900000 1.759810e-23 1.75981e-23 FALSE FALSE
ENST00000527947 ENSG00000101146 HEK293_OSMI2_2hA HEK293_TMG_2hB RAE1 protein_coding protein_coding 65.97402 73.96653 61.79879 7.224566 0.7559548 -0.2592557 23.864893 20.241331 24.751195 1.2902827 1.6444125 0.2900642 0.37068333 0.27780000 0.40053333 0.122733333 5.433318e-02 1.75981e-23 FALSE TRUE
MSTRG.20894.1 ENSG00000101146 HEK293_OSMI2_2hA HEK293_TMG_2hB RAE1 protein_coding   65.97402 73.96653 61.79879 7.224566 0.7559548 -0.2592557 3.807671 4.428702 3.941175 0.2308565 0.3209221 -0.1678559 0.05865000 0.06080000 0.06383333 0.003033333 9.121734e-01 1.75981e-23 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000101146 E001 1.5049804 0.0246771169 2.870384e-01 4.274976e-01 20 57351155 57351222 68 + 0.490 0.304 -1.043
ENSG00000101146 E002 45.6554855 0.0012172180 5.172748e-11 9.587728e-10 20 57351223 57351251 29 + 1.840 1.500 -1.155
ENSG00000101146 E003 47.2753861 0.0008454420 9.213235e-11 1.636126e-09 20 57351252 57351254 3 + 1.848 1.524 -1.101
ENSG00000101146 E004 132.1826366 0.0002922174 2.623843e-08 2.989230e-07 20 57351255 57351273 19 + 2.206 2.042 -0.549
ENSG00000101146 E005 332.6655826 0.0018721585 8.365256e-05 4.452734e-04 20 57351274 57351358 85 + 2.568 2.462 -0.355
ENSG00000101146 E006 343.8247060 0.0014445262 6.971589e-05 3.786110e-04 20 57351359 57351422 64 + 2.578 2.480 -0.328
ENSG00000101146 E007 0.6902858 0.5174320495 8.135900e-01 8.818286e-01 20 57351510 57351543 34 + 0.151 0.247 0.880
ENSG00000101146 E008 1.0621005 0.0236531719 2.785957e-01 4.182644e-01 20 57351544 57351570 27 + 0.152 0.355 1.599
ENSG00000101146 E009 0.1472490 0.0438571147 2.991586e-01   20 57351571 57351576 6 + 0.152 0.000 -11.100
ENSG00000101146 E010 0.7352129 0.0892726760 6.807161e-02 1.401659e-01 20 57351577 57351890 314 + 0.427 0.098 -2.731
ENSG00000101146 E011 0.0000000       20 57351891 57351919 29 +      
ENSG00000101146 E012 0.1482932 0.0406498416 7.657324e-01   20 57351920 57351998 79 + 0.000 0.098 9.372
ENSG00000101146 E013 484.9281370 0.0017675871 4.739898e-03 1.520522e-02 20 57354032 57354128 97 + 2.707 2.639 -0.226
ENSG00000101146 E014 547.3548421 0.0001899848 2.825795e-05 1.691005e-04 20 57354712 57354816 105 + 2.756 2.699 -0.192
ENSG00000101146 E015 603.4201676 0.0001284191 1.094450e-03 4.285216e-03 20 57356446 57356538 93 + 2.787 2.749 -0.128
ENSG00000101146 E016 682.5501975 0.0002395818 2.407283e-02 5.996274e-02 20 57365356 57365442 87 + 2.831 2.808 -0.078
ENSG00000101146 E017 597.5384017 0.0003620031 5.125690e-01 6.474633e-01 20 57366807 57366893 87 + 2.744 2.766 0.073
ENSG00000101146 E018 496.8124546 0.0001315658 5.610811e-01 6.889755e-01 20 57367008 57367079 72 + 2.663 2.684 0.068
ENSG00000101146 E019 565.6762362 0.0012351881 4.331503e-01 5.755903e-01 20 57368705 57368812 108 + 2.715 2.737 0.073
ENSG00000101146 E020 89.1953755 0.0146864929 2.022490e-07 1.936004e-06 20 57371134 57373474 2341 + 2.136 1.770 -1.230
ENSG00000101146 E021 708.5455126 0.0006004047 3.171614e-02 7.529766e-02 20 57373475 57373581 107 + 2.799 2.843 0.147
ENSG00000101146 E022 7.7466165 0.0022095074 1.626914e-01 2.786669e-01 20 57373582 57373662 81 + 1.026 0.879 -0.550
ENSG00000101146 E023 699.6969964 0.0001575269 2.637552e-06 1.999025e-05 20 57373663 57373738 76 + 2.772 2.852 0.265
ENSG00000101146 E024 919.9506632 0.0009571292 3.798660e-07 3.440557e-06 20 57374607 57374801 195 + 2.873 2.975 0.338
ENSG00000101146 E025 13.1753747 0.0195687232 1.641919e-04 8.101276e-04 20 57374802 57375106 305 + 1.358 0.946 -1.478
ENSG00000101146 E026 31.6763363 0.0077086751 3.567813e-01 5.011656e-01 20 57376179 57376266 88 + 1.437 1.511 0.256
ENSG00000101146 E027 1023.7159618 0.0015832959 2.975590e-10 4.846674e-09 20 57378013 57379211 1199 + 2.890 3.030 0.464