ENSG00000101132

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000371419 ENSG00000101132 HEK293_OSMI2_2hA HEK293_TMG_2hB PFDN4 protein_coding protein_coding 48.06696 24.51652 91.83322 4.68836 3.743775 1.904831 2.48067 2.856217 3.263137 0.7481859 0.07441996 0.1915263 0.06288333 0.1137667 0.03566667 -0.0781 1.755528e-13 1.755528e-13 FALSE  
ENST00000441080 ENSG00000101132 HEK293_OSMI2_2hA HEK293_TMG_2hB PFDN4 protein_coding nonsense_mediated_decay 48.06696 24.51652 91.83322 4.68836 3.743775 1.904831 45.27609 21.531121 88.045727 3.9444439 3.83107551 2.0313235 0.92933750 0.8806333 0.95853333 0.0779 1.184398e-11 1.755528e-13 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000101132 E001 12.2354524 0.0123696929 1.685165e-01 2.862352e-01 20 54208087 54208094 8 + 1.033 1.179 0.524
ENSG00000101132 E002 181.6655413 0.0002209783 1.642920e-02 4.367816e-02 20 54208095 54208124 30 + 2.249 2.182 -0.224
ENSG00000101132 E003 1.7089498 0.0979554054 9.194474e-01 9.531203e-01 20 54208300 54208311 12 + 0.410 0.418 0.041
ENSG00000101132 E004 3.3739785 0.0523727090 4.878928e-01 6.255276e-01 20 54208312 54208427 116 + 0.574 0.681 0.465
ENSG00000101132 E005 1.2147514 0.0106492932 3.423161e-01 4.863139e-01 20 54208428 54208442 15 + 0.375 0.188 -1.344
ENSG00000101132 E006 1.0759214 0.0124814736 4.473792e-01 5.887865e-01 20 54208443 54208558 116 + 0.338 0.188 -1.122
ENSG00000101132 E007 1.4906000 0.2093610631 2.972143e-01 4.385230e-01 20 54208835 54208915 81 + 0.442 0.186 -1.728
ENSG00000101132 E008 338.6904995 0.0001855527 9.473851e-02 1.824811e-01 20 54214351 54214458 108 + 2.509 2.477 -0.107
ENSG00000101132 E009 491.1476460 0.0001414721 3.742456e-02 8.626737e-02 20 54215300 54215440 141 + 2.670 2.640 -0.101
ENSG00000101132 E010 342.9706240 0.0001541885 2.599994e-01 3.974803e-01 20 54219019 54219145 127 + 2.500 2.522 0.072
ENSG00000101132 E011 56.1110120 0.0004758730 5.712513e-22 4.535702e-20 20 54219146 54219961 816 + 1.543 1.992 1.518
ENSG00000101132 E012 1.3138274 0.2348511422 4.086672e-01 5.522836e-01 20 54224227 54224299 73 + 0.408 0.186 -1.547
ENSG00000101132 E013 0.1482932 0.0411597534 1.669658e-01   20 54227953 54228052 100 + 0.000 0.187 10.305