ENSG00000101003

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262460 ENSG00000101003 HEK293_OSMI2_2hA HEK293_TMG_2hB GINS1 protein_coding protein_coding 22.75981 14.51991 37.0107 4.246317 1.214826 1.349306 11.163635 8.8637416 18.1199586 1.7042646 1.5407009 1.0307612 0.51714583 0.64296667 0.48793333 -0.15503333 1.863411e-01 2.654041e-20 FALSE TRUE
ENST00000696793 ENSG00000101003 HEK293_OSMI2_2hA HEK293_TMG_2hB GINS1 protein_coding nonsense_mediated_decay 22.75981 14.51991 37.0107 4.246317 1.214826 1.349306 2.913096 0.3072743 5.4837754 0.3072743 0.5358580 4.1139954 0.10147083 0.01336667 0.14846667 0.13510000 2.741773e-02 2.654041e-20 TRUE TRUE
ENST00000696804 ENSG00000101003 HEK293_OSMI2_2hA HEK293_TMG_2hB GINS1 protein_coding protein_coding 22.75981 14.51991 37.0107 4.246317 1.214826 1.349306 2.013884 0.0000000 3.6243822 0.0000000 0.4769858 8.5055663 0.05871667 0.00000000 0.09730000 0.09730000 2.654041e-20 2.654041e-20 FALSE TRUE
ENST00000696806 ENSG00000101003 HEK293_OSMI2_2hA HEK293_TMG_2hB GINS1 protein_coding protein_coding 22.75981 14.51991 37.0107 4.246317 1.214826 1.349306 1.056024 1.6176379 0.8753866 0.5483971 0.1046006 -0.8784002 0.05854583 0.11013333 0.02386667 -0.08626667 2.313196e-05 2.654041e-20 FALSE FALSE
ENST00000696870 ENSG00000101003 HEK293_OSMI2_2hA HEK293_TMG_2hB GINS1 protein_coding nonsense_mediated_decay 22.75981 14.51991 37.0107 4.246317 1.214826 1.349306 1.420253 0.0000000 3.2469074 0.0000000 0.9026986 8.3473589 0.04153333 0.00000000 0.08853333 0.08853333 1.363699e-08 2.654041e-20   FALSE
ENST00000696875 ENSG00000101003 HEK293_OSMI2_2hA HEK293_TMG_2hB GINS1 protein_coding protein_coding 22.75981 14.51991 37.0107 4.246317 1.214826 1.349306 1.079059 1.8295798 0.5039933 1.8295798 0.1388445 -1.8395548 0.07203750 0.07950000 0.01340000 -0.06610000 7.776485e-01 2.654041e-20   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000101003 E001 0.4386386 0.0747323668 1.000000e+00 1.000000e+00 20 25391008 25391198 191 + 0.163 0.165 0.013
ENSG00000101003 E002 0.1472490 0.0507007693 1.000000e+00   20 25407611 25407672 62 + 0.089 0.000 -8.990
ENSG00000101003 E003 0.1472490 0.0507007693 1.000000e+00   20 25407673 25407675 3 + 0.089 0.000 -8.990
ENSG00000101003 E004 0.6717251 0.0534301838 7.458302e-01 8.337481e-01 20 25407676 25407682 7 + 0.227 0.166 -0.562
ENSG00000101003 E005 0.9651910 0.0121989077 9.587800e-01 9.781677e-01 20 25407683 25407684 2 + 0.282 0.285 0.019
ENSG00000101003 E006 1.4435834 0.0096765512 9.515082e-01 9.736646e-01 20 25407685 25407685 1 + 0.375 0.378 0.019
ENSG00000101003 E007 2.0733896 0.0077268123 3.895428e-01 5.336176e-01 20 25407686 25407687 2 + 0.415 0.577 0.795
ENSG00000101003 E008 7.3756631 0.0141028429 5.781251e-01 7.032856e-01 20 25407688 25407697 10 + 0.932 0.874 -0.221
ENSG00000101003 E009 14.6935789 0.0015177807 5.945768e-02 1.257054e-01 20 25407698 25407701 4 + 1.236 1.083 -0.547
ENSG00000101003 E010 18.6469910 0.0011873079 1.464995e-02 3.970627e-02 20 25407702 25407706 5 + 1.343 1.159 -0.648
ENSG00000101003 E011 19.6778599 0.0009455475 1.380183e-02 3.777712e-02 20 25407707 25407708 2 + 1.365 1.186 -0.628
ENSG00000101003 E012 40.4920040 0.0137811747 1.793511e-02 4.699585e-02 20 25407709 25407728 20 + 1.670 1.466 -0.696
ENSG00000101003 E013 41.2346038 0.0073699106 5.552672e-03 1.742599e-02 20 25407729 25407730 2 + 1.676 1.479 -0.672
ENSG00000101003 E014 48.3897416 0.0016195805 1.475586e-04 7.365760e-04 20 25407731 25407738 8 + 1.745 1.547 -0.674
ENSG00000101003 E015 81.2797998 0.0043865685 2.969044e-06 2.223754e-05 20 25407739 25407768 30 + 1.980 1.736 -0.821
ENSG00000101003 E016 146.5312927 0.0005836823 2.733128e-17 1.260426e-15 20 25407769 25407895 127 + 2.241 1.977 -0.883
ENSG00000101003 E017 4.6448162 0.1047572626 7.604290e-01 8.445488e-01 20 25411124 25411286 163 + 0.763 0.679 -0.349
ENSG00000101003 E018 2.6173318 0.0680608976 2.261279e-01 3.577630e-01 20 25413457 25413789 333 + 0.452 0.673 1.019
ENSG00000101003 E019 123.6853956 0.0002992747 4.369213e-18 2.225092e-16 20 25413790 25413854 65 + 2.173 1.888 -0.954
ENSG00000101003 E020 0.6590396 0.0362186602 7.407065e-01 8.300600e-01 20 25413855 25413933 79 + 0.227 0.166 -0.562
ENSG00000101003 E021 91.7726223 0.0012463972 1.004796e-10 1.771903e-09 20 25417104 25417140 37 + 2.040 1.779 -0.877
ENSG00000101003 E022 91.8464129 0.0003363558 1.649907e-16 6.910679e-15 20 25417141 25417202 62 + 2.051 1.731 -1.080
ENSG00000101003 E023 2.9224284 0.0558780853 5.226949e-01 6.564508e-01 20 25417203 25418104 902 + 0.623 0.519 -0.474
ENSG00000101003 E024 111.8631192 0.0022838547 3.476778e-08 3.866767e-07 20 25418105 25418195 91 + 2.114 1.892 -0.748
ENSG00000101003 E025 6.5115108 0.0025939246 4.229732e-01 5.661007e-01 20 25419684 25419805 122 + 0.895 0.817 -0.304
ENSG00000101003 E026 0.4460135 0.0503365098 2.755108e-01 4.148306e-01 20 25420846 25420848 3 + 0.227 0.000 -10.569
ENSG00000101003 E027 3.7123595 0.0611549091 1.137267e-01 2.109583e-01 20 25420849 25420988 140 + 0.747 0.456 -1.303
ENSG00000101003 E028 6.3436389 0.0449196625 1.175886e-01 2.165024e-01 20 25423505 25425210 1706 + 0.932 0.673 -1.028
ENSG00000101003 E029 134.3957244 0.0019327607 8.388673e-06 5.671695e-05 20 25425211 25425327 117 + 2.176 2.021 -0.518
ENSG00000101003 E030 2.2875392 0.0060658888 1.150728e-02 3.236707e-02 20 25427211 25427292 82 + 0.623 0.165 -2.788
ENSG00000101003 E031 1.5176804 0.0090375494 7.651287e-01 8.479662e-01 20 25427293 25427340 48 + 0.415 0.378 -0.205
ENSG00000101003 E032 129.1430841 0.0002343750 2.014221e-09 2.816772e-08 20 25441702 25441776 75 + 2.166 1.992 -0.582
ENSG00000101003 E033 0.2955422 0.0275995554 6.301460e-01   20 25443009 25443023 15 + 0.089 0.165 1.016
ENSG00000101003 E034 0.5943067 0.0223804001 7.208642e-01 8.153946e-01 20 25443024 25443115 92 + 0.227 0.165 -0.569
ENSG00000101003 E035 193.1470189 0.0002093330 9.747851e-17 4.196094e-15 20 25445923 25446039 117 + 2.347 2.144 -0.679
ENSG00000101003 E036 766.1739078 0.0001127328 9.504404e-06 6.350135e-05 20 25446040 25447090 1051 + 2.890 2.860 -0.100
ENSG00000101003 E037 498.9906041 0.0002495317 7.634820e-17 3.333473e-15 20 25447091 25447685 595 + 2.628 2.787 0.530
ENSG00000101003 E038 332.3126274 0.0027862651 2.893287e-11 5.589115e-10 20 25447686 25447922 237 + 2.415 2.648 0.776
ENSG00000101003 E039 310.7570192 0.0065647967 3.654590e-05 2.126124e-04 20 25447923 25448102 180 + 2.395 2.605 0.699
ENSG00000101003 E040 139.0603690 0.0008186563 8.636955e-10 1.294908e-08 20 25448103 25448120 18 + 2.048 2.273 0.754
ENSG00000101003 E041 351.8617336 0.0003106144 5.641331e-43 2.333478e-40 20 25448121 25449072 952 + 2.413 2.708 0.981
ENSG00000101003 E042 1.0447003 0.0942831844 2.818873e-01 4.218389e-01 20 25452442 25452700 259 + 0.375 0.165 -1.577