ENSG00000101000

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216968 ENSG00000101000 HEK293_OSMI2_2hA HEK293_TMG_2hB PROCR protein_coding protein_coding 5.100023 7.688177 4.886098 0.3092077 0.1113498 -0.6528844 4.5833340 6.326835 4.6141506 0.2464163 0.1923475 -0.454574 0.9086292 0.8237333 0.9436 0.1198667 0.01598927 0.01598927 FALSE TRUE
MSTRG.20617.1 ENSG00000101000 HEK293_OSMI2_2hA HEK293_TMG_2hB PROCR protein_coding   5.100023 7.688177 4.886098 0.3092077 0.1113498 -0.6528844 0.3080304 1.147088 0.1308757 0.2107050 0.1308757 -3.038005 0.0522750 0.1484667 0.0276 -0.1208667 0.10777499 0.01598927 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000101000 E001 1.1414950 0.0123935775 0.213325023 0.34244908 20 35171866 35171894 29 + 0.158 0.389 1.727
ENSG00000101000 E002 5.5489809 0.0038952866 0.313006179 0.45552238 20 35172072 35172088 17 + 0.879 0.748 -0.514
ENSG00000101000 E003 38.9966696 0.0008554306 0.919888653 0.95340420 20 35172089 35172224 136 + 1.572 1.566 -0.020
ENSG00000101000 E004 26.6554013 0.0007413812 0.313790260 0.45642504 20 35174702 35174731 30 + 1.456 1.389 -0.232
ENSG00000101000 E005 61.8783455 0.0004468269 0.542449430 0.67320941 20 35174732 35174953 222 + 1.785 1.759 -0.089
ENSG00000101000 E006 58.1702869 0.0006105087 0.956840875 0.97692592 20 35176168 35176352 185 + 1.746 1.744 -0.008
ENSG00000101000 E007 53.0018605 0.0005604326 0.155490523 0.26904267 20 35176353 35176446 94 + 1.655 1.724 0.234
ENSG00000101000 E008 107.2391134 0.0002907297 0.008042396 0.02388549 20 35176698 35177086 389 + 1.945 2.030 0.286
ENSG00000101000 E009 44.4349061 0.0005303125 0.067915261 0.13990934 20 35177087 35177215 129 + 1.691 1.599 -0.315
ENSG00000101000 E010 19.4662913 0.0010387861 0.032379802 0.07658821 20 35177216 35177362 147 + 1.391 1.229 -0.566
ENSG00000101000 E011 0.3686942 0.0273085867 0.668496443 0.77547423 20 35215893 35216240 348 + 0.158 0.094 -0.852