ENSG00000100997

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000339157 ENSG00000100997 HEK293_OSMI2_2hA HEK293_TMG_2hB ABHD12 protein_coding protein_coding 58.59731 98.70558 36.62865 2.082746 0.1752646 -1.429912 34.719128 47.901503 27.427298 5.4189839 0.8849716 -0.8042336 0.5918250 0.483600000 0.74900000 0.26540000 5.459860e-04 2.452953e-60 FALSE TRUE
ENST00000376542 ENSG00000100997 HEK293_OSMI2_2hA HEK293_TMG_2hB ABHD12 protein_coding protein_coding 58.59731 98.70558 36.62865 2.082746 0.1752646 -1.429912 2.061054 0.961259 2.459005 0.6581023 0.4831453 1.3460021 0.0412125 0.009733333 0.06720000 0.05746667 1.697352e-01 2.452953e-60 FALSE TRUE
ENST00000672258 ENSG00000100997 HEK293_OSMI2_2hA HEK293_TMG_2hB ABHD12 protein_coding protein_coding 58.59731 98.70558 36.62865 2.082746 0.1752646 -1.429912 3.465663 13.457843 0.000000 0.4779098 0.0000000 -10.3953031 0.0409125 0.136466667 0.00000000 -0.13646667 2.452953e-60 2.452953e-60 FALSE TRUE
ENST00000673524 ENSG00000100997 HEK293_OSMI2_2hA HEK293_TMG_2hB ABHD12 protein_coding nonsense_mediated_decay 58.59731 98.70558 36.62865 2.082746 0.1752646 -1.429912 8.234453 24.339242 1.264045 2.3509552 0.6526360 -4.2563890 0.1282083 0.247400000 0.03436667 -0.21303333 1.636469e-01 2.452953e-60 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100997 E001 0.1451727 4.347206e-02 1.655487e-01   20 25294742 25294742 1 - 0.192 0.000 -11.697
ENSG00000100997 E002 0.6234634 1.850527e-02 1.000000e+00 1.000000e+00 20 25294743 25294746 4 - 0.192 0.196 0.038
ENSG00000100997 E003 47.1097231 3.817855e-02 2.517213e-01 3.879894e-01 20 25294747 25295030 284 - 1.702 1.575 -0.434
ENSG00000100997 E004 2.6882159 7.598461e-03 1.922727e-01 3.166140e-01 20 25300213 25300213 1 - 0.325 0.564 1.261
ENSG00000100997 E005 10.3221844 3.732171e-03 3.778545e-01 5.221559e-01 20 25300214 25300216 3 - 1.063 0.954 -0.400
ENSG00000100997 E006 119.5467545 8.490143e-04 2.291112e-01 3.613663e-01 20 25300217 25300254 38 - 2.045 1.989 -0.188
ENSG00000100997 E007 354.1601604 2.163597e-03 2.783077e-02 6.768149e-02 20 25300255 25300365 111 - 2.420 2.482 0.206
ENSG00000100997 E008 379.1454762 1.052143e-03 4.176649e-04 1.842138e-03 20 25300366 25300428 63 - 2.431 2.517 0.284
ENSG00000100997 E009 857.5717256 9.070502e-05 6.923333e-22 5.437830e-20 20 25300429 25300698 270 - 2.743 2.877 0.444
ENSG00000100997 E010 310.6637240 5.331772e-04 5.783923e-08 6.146213e-07 20 25300699 25300702 4 - 2.303 2.439 0.453
ENSG00000100997 E011 669.8690332 1.402491e-04 4.296048e-12 9.496773e-11 20 25300703 25300832 130 - 2.656 2.765 0.360
ENSG00000100997 E012 493.1028424 4.902281e-04 6.565607e-04 2.739843e-03 20 25300833 25300884 52 - 2.561 2.624 0.212
ENSG00000100997 E013 7.3593549 1.088376e-02 4.100610e-01 5.536866e-01 20 25301249 25301294 46 - 0.944 0.826 -0.448
ENSG00000100997 E014 9.6434803 2.659687e-02 1.494057e-01 2.608514e-01 20 25301332 25301378 47 - 1.103 0.912 -0.705
ENSG00000100997 E015 12.9493399 1.435306e-03 2.279316e-04 1.082989e-03 20 25301719 25301833 115 - 1.322 0.963 -1.288
ENSG00000100997 E016 21.4157085 8.949048e-04 3.653418e-06 2.683399e-05 20 25301834 25302034 201 - 1.522 1.157 -1.272
ENSG00000100997 E017 527.4536048 1.753269e-04 1.321078e-01 2.370425e-01 20 25302219 25302276 58 - 2.627 2.645 0.060
ENSG00000100997 E018 508.0149417 6.868543e-04 3.634669e-01 5.078752e-01 20 25302277 25302346 70 - 2.617 2.628 0.035
ENSG00000100997 E019 4.8261971 8.076964e-02 9.177614e-01 9.520081e-01 20 25303263 25303549 287 - 0.689 0.700 0.046
ENSG00000100997 E020 383.6114040 1.266057e-03 3.114321e-01 4.538872e-01 20 25303550 25303577 28 - 2.490 2.508 0.061
ENSG00000100997 E021 490.9680676 8.205592e-04 6.266342e-01 7.425420e-01 20 25303578 25303628 51 - 2.609 2.611 0.007
ENSG00000100997 E022 3.2053949 5.191153e-03 4.931192e-02 1.079407e-01 20 25306299 25306540 242 - 0.778 0.463 -1.391
ENSG00000100997 E023 500.2939548 1.169685e-04 6.533813e-01 7.638736e-01 20 25306833 25306915 83 - 2.618 2.616 -0.006
ENSG00000100997 E024 432.5118550 1.565496e-04 6.528991e-01 7.635171e-01 20 25307966 25308045 80 - 2.568 2.549 -0.064
ENSG00000100997 E025 338.3415302 3.008523e-04 7.071450e-02 1.445302e-01 20 25308457 25308494 38 - 2.486 2.434 -0.173
ENSG00000100997 E026 378.3044957 6.754448e-04 1.892463e-03 6.890667e-03 20 25309446 25309498 53 - 2.558 2.475 -0.277
ENSG00000100997 E027 403.7046499 1.620287e-04 1.684851e-08 1.991328e-07 20 25309499 25309575 77 - 2.616 2.493 -0.410
ENSG00000100997 E028 2.1390661 8.862212e-03 1.249824e-01 2.271031e-01 20 25310200 25310472 273 - 0.637 0.368 -1.322
ENSG00000100997 E029 250.2421739 1.812018e-04 3.367213e-07 3.083172e-06 20 25314925 25314970 46 - 2.421 2.283 -0.461
ENSG00000100997 E030 223.5586308 1.543971e-03 3.080102e-04 1.412256e-03 20 25317048 25317078 31 - 2.363 2.237 -0.420
ENSG00000100997 E031 3.6782086 1.414036e-02 5.373594e-02 1.157923e-01 20 25319052 25319155 104 - 0.325 0.681 1.771
ENSG00000100997 E032 0.1482932 4.148070e-02 1.000000e+00   20 25319773 25319825 53 - 0.000 0.076 11.054
ENSG00000100997 E033 393.3075259 1.185405e-03 7.971572e-03 2.370599e-02 20 25320199 25320318 120 - 2.572 2.494 -0.259
ENSG00000100997 E034 420.6134481 2.283310e-03 7.301938e-03 2.200519e-02 20 25323325 25323430 106 - 2.610 2.521 -0.296
ENSG00000100997 E035 8.6990147 2.071733e-03 2.887884e-01 4.294222e-01 20 25338885 25339004 120 - 1.019 0.885 -0.503
ENSG00000100997 E036 3.4298734 4.702660e-03 2.298635e-01 3.622933e-01 20 25339220 25339226 7 - 0.736 0.541 -0.845
ENSG00000100997 E037 342.4025080 2.913191e-03 5.403966e-03 1.702523e-02 20 25339227 25339351 125 - 2.530 2.428 -0.339
ENSG00000100997 E038 0.1472490 4.305329e-02 1.656564e-01   20 25339622 25339694 73 - 0.192 0.000 -13.559
ENSG00000100997 E039 0.1817044 4.053393e-02 1.000000e+00   20 25339695 25339749 55 - 0.000 0.076 11.068
ENSG00000100997 E040 0.0000000       20 25341946 25342096 151 -      
ENSG00000100997 E041 1.1876934 1.450867e-01 4.363831e-01 5.784741e-01 20 25389926 25390387 462 - 0.427 0.247 -1.126
ENSG00000100997 E042 154.3568148 2.050695e-02 3.103036e-07 2.862169e-06 20 25390513 25390839 327 - 2.377 1.988 -1.301