ENSG00000100994

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216962 ENSG00000100994 HEK293_OSMI2_2hA HEK293_TMG_2hB PYGB protein_coding protein_coding 47.04446 84.2297 24.57715 3.047425 1.08836 -1.776596 34.016106 54.861674 20.4932946 4.9301912 0.8315001 -1.420206 0.76456250 0.64956667 0.834166667 0.18460000 1.207620e-03 1.627216e-06 FALSE TRUE
ENST00000428458 ENSG00000100994 HEK293_OSMI2_2hA HEK293_TMG_2hB PYGB protein_coding protein_coding 47.04446 84.2297 24.57715 3.047425 1.08836 -1.776596 4.716560 14.740785 0.0255273 4.8293793 0.0255273 -8.697648 0.06031667 0.17663333 0.001033333 -0.17560000 1.627216e-06 1.627216e-06 FALSE TRUE
ENST00000471359 ENSG00000100994 HEK293_OSMI2_2hA HEK293_TMG_2hB PYGB protein_coding processed_transcript 47.04446 84.2297 24.57715 3.047425 1.08836 -1.776596 4.642988 7.597608 2.5449091 0.3959408 0.2200732 -1.574171 0.09988333 0.09053333 0.103166667 0.01263333 6.556559e-01 1.627216e-06   FALSE
MSTRG.20492.1 ENSG00000100994 HEK293_OSMI2_2hA HEK293_TMG_2hB PYGB protein_coding   47.04446 84.2297 24.57715 3.047425 1.08836 -1.776596 3.668807 7.029638 1.5134228 1.4005863 0.1990418 -2.208185 0.07523333 0.08330000 0.061600000 -0.02170000 6.205310e-01 1.627216e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100994 E001 27.0699564 0.0103897090 4.547832e-01 5.953791e-01 20 25248068 25248084 17 + 1.271 1.378 0.372
ENSG00000100994 E002 290.1803373 0.0101883325 3.967271e-02 9.042090e-02 20 25248085 25248421 337 + 2.429 2.360 -0.227
ENSG00000100994 E003 242.1439704 0.0034314777 3.919929e-04 1.743891e-03 20 25259237 25259338 102 + 2.370 2.273 -0.322
ENSG00000100994 E004 243.1039462 0.0004027219 1.029684e-05 6.826379e-05 20 25269129 25269207 79 + 2.362 2.275 -0.290
ENSG00000100994 E005 279.4265033 0.0015303354 1.020915e-02 2.928137e-02 20 25271383 25271486 104 + 2.391 2.348 -0.143
ENSG00000100994 E006 364.5740970 0.0017880980 6.258146e-03 1.928976e-02 20 25274592 25274723 132 + 2.508 2.464 -0.145
ENSG00000100994 E007 362.6301040 0.0007596327 2.317966e-05 1.415960e-04 20 25276646 25276757 112 + 2.524 2.454 -0.234
ENSG00000100994 E008 334.3274530 0.0021555244 4.520970e-05 2.574808e-04 20 25277244 25277326 83 + 2.505 2.414 -0.301
ENSG00000100994 E009 425.9975687 0.0047344041 2.698883e-02 6.597274e-02 20 25278319 25278462 144 + 2.577 2.531 -0.154
ENSG00000100994 E010 360.8335509 0.0056979580 1.043846e-01 1.971639e-01 20 25279057 25279149 93 + 2.494 2.463 -0.104
ENSG00000100994 E011 234.6535250 0.0026476719 3.060958e-04 1.404124e-03 20 25280266 25280283 18 + 2.353 2.259 -0.314
ENSG00000100994 E012 392.0931484 0.0018457139 8.090406e-02 1.609492e-01 20 25280284 25280412 129 + 2.515 2.500 -0.048
ENSG00000100994 E013 437.0194703 0.0011780248 4.548392e-01 5.954229e-01 20 25280949 25281112 164 + 2.538 2.555 0.055
ENSG00000100994 E014 363.0101203 0.0001519974 1.550085e-03 5.798037e-03 20 25282033 25282147 115 + 2.499 2.462 -0.121
ENSG00000100994 E015 431.6722030 0.0001996247 4.849029e-02 1.064935e-01 20 25283176 25283277 102 + 2.552 2.545 -0.023
ENSG00000100994 E016 488.0695764 0.0001517374 5.375973e-01 6.691295e-01 20 25284104 25284251 148 + 2.582 2.604 0.073
ENSG00000100994 E017 366.5648790 0.0001689998 4.517748e-01 5.926980e-01 20 25288425 25288483 59 + 2.461 2.478 0.057
ENSG00000100994 E018 0.7697675 0.0328029758 9.415269e-01 9.672282e-01 20 25289848 25289930 83 + 0.196 0.193 -0.027
ENSG00000100994 E019 489.3650889 0.0001441732 7.222309e-01 8.163951e-01 20 25290481 25290622 142 + 2.578 2.605 0.090
ENSG00000100994 E020 626.5767342 0.0003584209 4.786225e-01 6.170413e-01 20 25292406 25292613 208 + 2.668 2.716 0.162
ENSG00000100994 E021 50.4779547 0.0004505951 2.602732e-12 5.975275e-11 20 25293901 25294157 257 + 1.863 1.530 -1.129
ENSG00000100994 E022 551.9387401 0.0001735129 4.527911e-01 5.936007e-01 20 25294158 25294292 135 + 2.613 2.661 0.162
ENSG00000100994 E023 421.9203253 0.0002253706 3.350044e-01 4.787470e-01 20 25295604 25295670 67 + 2.490 2.545 0.185
ENSG00000100994 E024 3230.0511334 0.0031796466 3.015716e-11 5.807852e-10 20 25296370 25298305 1936 + 3.270 3.455 0.613