ENSG00000100982

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000372409 ENSG00000100982 HEK293_OSMI2_2hA HEK293_TMG_2hB PCIF1 protein_coding protein_coding 23.49911 40.48498 17.42342 1.251788 0.6897379 -1.215887 17.513019 28.470506 13.443245 1.4582847 0.5240803 -1.082021 0.7624583 0.7023667 0.7717 0.06933333 0.07675074 0.0253769 FALSE  
ENST00000616084 ENSG00000100982 HEK293_OSMI2_2hA HEK293_TMG_2hB PCIF1 protein_coding processed_transcript 23.49911 40.48498 17.42342 1.251788 0.6897379 -1.215887 4.548254 9.603384 2.653745 0.3548863 0.3454971 -1.851588 0.1759292 0.2381667 0.1512 -0.08696667 0.02537690 0.0253769    
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100982 E001 2.318565 0.0065799707 2.523310e-01 0.3886641430 20 45933009 45934682 1674 + 0.304 0.518 1.176
ENSG00000100982 E002 67.614711 0.0029721914 5.068893e-01 0.6425212971 20 45934683 45934732 50 + 1.743 1.781 0.128
ENSG00000100982 E003 118.220978 0.0009724224 5.772718e-01 0.7025453098 20 45934733 45934804 72 + 1.993 2.017 0.079
ENSG00000100982 E004 214.609920 0.0003547309 9.743457e-01 0.9879884602 20 45937418 45937585 168 + 2.271 2.274 0.009
ENSG00000100982 E005 76.534072 0.0033912643 5.897467e-05 0.0003263876 20 45937586 45937972 387 + 1.650 1.879 0.776
ENSG00000100982 E006 170.462053 0.0027071158 1.478740e-01 0.2587925128 20 45938981 45939123 143 + 2.213 2.161 -0.174
ENSG00000100982 E007 181.289125 0.0017101773 2.715302e-01 0.4103101092 20 45939215 45939339 125 + 2.228 2.193 -0.118
ENSG00000100982 E008 193.257862 0.0002594910 5.687388e-02 0.1212441670 20 45940475 45940612 138 + 2.265 2.214 -0.173
ENSG00000100982 E009 191.531286 0.0002336149 2.184756e-03 0.0077946694 20 45940809 45940939 131 + 2.284 2.201 -0.279
ENSG00000100982 E010 194.991181 0.0011299064 1.033693e-02 0.0295981150 20 45941053 45941207 155 + 2.284 2.206 -0.259
ENSG00000100982 E011 206.065049 0.0010257237 2.013013e-04 0.0009693195 20 45943097 45943244 148 + 2.331 2.222 -0.363
ENSG00000100982 E012 142.684890 0.0015655203 1.850834e-01 0.3076014556 20 45943340 45943423 84 + 2.129 2.082 -0.157
ENSG00000100982 E013 79.791310 0.0004190462 5.417768e-01 0.6727052134 20 45943666 45943668 3 + 1.861 1.835 -0.086
ENSG00000100982 E014 159.497999 0.0014543739 4.398129e-01 0.5815501612 20 45943669 45943765 97 + 2.160 2.135 -0.084
ENSG00000100982 E015 10.145374 0.0049250747 3.754471e-01 0.5198833901 20 45944245 45944320 76 + 0.909 1.017 0.402
ENSG00000100982 E016 205.317694 0.0006877226 6.450157e-01 0.7572335557 20 45944868 45945006 139 + 2.241 2.257 0.053
ENSG00000100982 E017 111.132978 0.0026136358 3.265152e-01 0.4699511722 20 45945007 45945030 24 + 1.956 2.003 0.158
ENSG00000100982 E018 222.712022 0.0001771982 6.527120e-02 0.1354904596 20 45945711 45945883 173 + 2.248 2.299 0.171
ENSG00000100982 E019 156.393216 0.0002473996 4.070773e-02 0.0923323365 20 45946029 45946115 87 + 2.082 2.150 0.227
ENSG00000100982 E020 267.692428 0.0012580051 3.090439e-01 0.4513335870 20 45946200 45946384 185 + 2.342 2.374 0.107
ENSG00000100982 E021 225.418362 0.0002381020 7.617420e-01 0.8455434006 20 45947073 45947166 94 + 2.297 2.290 -0.021
ENSG00000100982 E022 307.821859 0.0002171173 1.388996e-01 0.2465831424 20 45947263 45947438 176 + 2.400 2.436 0.119
ENSG00000100982 E023 6.015050 0.0026924339 7.619670e-01 0.8456870259 20 45947439 45947523 85 + 0.819 0.779 -0.157
ENSG00000100982 E024 367.691510 0.0023300085 2.497723e-03 0.0087563822 20 45947524 45948023 500 + 2.432 2.527 0.317