ENSG00000100968

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000250373 ENSG00000100968 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC4 protein_coding protein_coding 1.917768 2.674308 1.478329 0.1711021 0.2027705 -0.850857 0.59979527 1.31722000 0.26899200 0.03419055 0.05338675 -2.250112 0.27298750 0.49576667 0.20066667 -0.29510000 0.01609953 0.01609953 FALSE TRUE
ENST00000539237 ENSG00000100968 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC4 protein_coding protein_coding 1.917768 2.674308 1.478329 0.1711021 0.2027705 -0.850857 0.08145085 0.15657883 0.02822246 0.04620471 0.02822246 -2.123713 0.07859583 0.06113333 0.01716667 -0.04396667 0.36944208 0.01609953 FALSE TRUE
ENST00000553708 ENSG00000100968 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC4 protein_coding protein_coding 1.917768 2.674308 1.478329 0.1711021 0.2027705 -0.850857 0.58724928 0.78952875 0.33188431 0.17085017 0.09540174 -1.225642 0.27832500 0.29066667 0.22130000 -0.06936667 0.69038542 0.01609953 FALSE TRUE
ENST00000554779 ENSG00000100968 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC4 protein_coding protein_coding 1.917768 2.674308 1.478329 0.1711021 0.2027705 -0.850857 0.07489265 0.07939169 0.43511049 0.07939169 0.22320249 2.315951 0.04739583 0.02643333 0.25980000 0.23336667 0.37138557 0.01609953 FALSE FALSE
ENST00000556302 ENSG00000100968 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC4 protein_coding retained_intron 1.917768 2.674308 1.478329 0.1711021 0.2027705 -0.850857 0.11421087 0.03949385 0.08702290 0.03949385 0.08702290 0.971076 0.06531667 0.01520000 0.05290000 0.03770000 0.92826662 0.01609953 TRUE TRUE
ENST00000556957 ENSG00000100968 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC4 protein_coding retained_intron 1.917768 2.674308 1.478329 0.1711021 0.2027705 -0.850857 0.06045491 0.03385549 0.11636721 0.03385549 0.11636721 1.526793 0.04033750 0.01126667 0.06783333 0.05656667 0.89507630 0.01609953 FALSE TRUE
ENST00000557028 ENSG00000100968 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC4 protein_coding retained_intron 1.917768 2.674308 1.478329 0.1711021 0.2027705 -0.850857 0.21346012 0.03957086 0.15021137 0.03957086 0.15021137 1.692412 0.11503750 0.01633333 0.13976667 0.12343333 0.94796035 0.01609953 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100968 E001 0.0000000       14 24365673 24365718 46 +      
ENSG00000100968 E002 0.0000000       14 24366911 24366938 28 +      
ENSG00000100968 E003 0.0000000       14 24366939 24367089 151 +      
ENSG00000100968 E004 0.0000000       14 24367090 24367214 125 +      
ENSG00000100968 E005 0.0000000       14 24367260 24367351 92 +      
ENSG00000100968 E006 0.1451727 0.0430409515 1.452971e-01   14 24367364 24367467 104 + 0.199 0.000 -10.971
ENSG00000100968 E007 0.3268771 0.0287616698 4.018913e-01   14 24367468 24367501 34 + 0.199 0.073 -1.682
ENSG00000100968 E008 1.2480582 0.0109077754 5.289643e-01 0.6618106438 14 24367502 24367653 152 + 0.200 0.320 0.903
ENSG00000100968 E009 0.0000000       14 24368020 24368047 28 +      
ENSG00000100968 E010 0.0000000       14 24368048 24368069 22 +      
ENSG00000100968 E011 0.4815130 0.0221194565 7.012699e-01 0.8005005842 14 24368070 24368169 100 + 0.200 0.134 -0.685
ENSG00000100968 E012 0.6287620 0.0181608244 2.655618e-01 0.4036286386 14 24368170 24368183 14 + 0.336 0.134 -1.684
ENSG00000100968 E013 0.6287620 0.0181608244 2.655618e-01 0.4036286386 14 24368184 24368185 2 + 0.336 0.134 -1.684
ENSG00000100968 E014 7.8878850 0.0022742165 6.927902e-05 0.0003765515 14 24368186 24368311 126 + 1.176 0.714 -1.743
ENSG00000100968 E015 8.2125840 0.0021758573 5.129629e-04 0.0022083690 14 24368312 24368340 29 + 1.159 0.758 -1.505
ENSG00000100968 E016 10.7731438 0.0017636003 3.316914e-02 0.0781430289 14 24368341 24368440 100 + 1.159 0.927 -0.849
ENSG00000100968 E017 0.0000000       14 24368999 24369081 83 +      
ENSG00000100968 E018 0.0000000       14 24369082 24369152 71 +      
ENSG00000100968 E019 0.0000000       14 24369153 24369156 4 +      
ENSG00000100968 E020 0.0000000       14 24369157 24369166 10 +      
ENSG00000100968 E021 0.0000000       14 24369167 24369244 78 +      
ENSG00000100968 E022 0.0000000       14 24369245 24369343 99 +      
ENSG00000100968 E023 0.0000000       14 24369344 24369376 33 +      
ENSG00000100968 E024 0.0000000       14 24369377 24369498 122 +      
ENSG00000100968 E025 10.7189182 0.0016591814 1.113931e-01 0.2075932219 14 24369499 24369670 172 + 1.122 0.945 -0.649
ENSG00000100968 E026 47.1219875 0.0005724107 5.850784e-01 0.7088470536 14 24369671 24370594 924 + 1.621 1.584 -0.126
ENSG00000100968 E027 0.1472490 0.0421934163 1.456330e-01   14 24371718 24371816 99 + 0.200 0.000 -13.334
ENSG00000100968 E028 0.2214452 0.0377501594 1.000000e+00   14 24372078 24372440 363 + 0.000 0.073 10.012
ENSG00000100968 E029 27.6870250 0.0009011448 2.786217e-01 0.4182736762 14 24372441 24372603 163 + 1.435 1.351 -0.293
ENSG00000100968 E030 0.0000000       14 24372815 24373170 356 +      
ENSG00000100968 E031 33.7076165 0.0038747972 6.661910e-01 0.7736739360 14 24373171 24373370 200 + 1.471 1.435 -0.123
ENSG00000100968 E032 29.9363722 0.0163711938 4.839770e-01 0.6220056770 14 24373695 24373867 173 + 1.453 1.379 -0.256
ENSG00000100968 E033 3.1815512 0.0058882935 1.876671e-05 0.0001171432 14 24373868 24374122 255 + 0.962 0.320 -2.905
ENSG00000100968 E034 2.4848460 0.0295430831 9.074607e-03 0.0264660786 14 24374123 24374325 203 + 0.796 0.355 -2.055
ENSG00000100968 E035 22.6732154 0.0110338569 8.191263e-01 0.8857869465 14 24374326 24374466 141 + 1.306 1.276 -0.107
ENSG00000100968 E036 0.6664396 0.5574676011 4.206234e-01 0.5638162545 14 24374467 24374707 241 + 0.337 0.136 -1.679
ENSG00000100968 E037 14.2093941 0.0013832452 4.377266e-01 0.5796264660 14 24375660 24375715 56 + 1.159 1.076 -0.299
ENSG00000100968 E038 23.1547288 0.0008769057 7.488730e-01 0.8359846906 14 24375975 24376073 99 + 1.319 1.287 -0.112
ENSG00000100968 E039 13.3204745 0.0014395856 4.983312e-01 0.6348035885 14 24376074 24376101 28 + 1.014 1.082 0.248
ENSG00000100968 E040 38.6895101 0.0007433913 3.645997e-03 0.0121439429 14 24376294 24376554 261 + 1.342 1.541 0.686
ENSG00000100968 E041 43.1498019 0.0005583606 1.886522e-05 0.0001176999 14 24376555 24376878 324 + 1.306 1.599 1.007
ENSG00000100968 E042 27.4944740 0.0022380691 6.350083e-01 0.7493812705 14 24376879 24377637 759 + 1.331 1.362 0.110
ENSG00000100968 E043 64.4681902 0.0004661866 1.714123e-05 0.0001079537 14 24377638 24379604 1967 + 1.535 1.762 0.774