ENSG00000100916

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216807 ENSG00000100916 HEK293_OSMI2_2hA HEK293_TMG_2hB BRMS1L protein_coding protein_coding 15.58793 5.79382 26.38532 0.6528629 0.713627 2.185208 12.732299 5.3108932 21.272891 0.6796833 1.3409053 1.999954 0.8503125 0.9130667 0.8047667 -0.1083 0.03567196 0.03567196 FALSE TRUE
ENST00000548758 ENSG00000100916 HEK293_OSMI2_2hA HEK293_TMG_2hB BRMS1L protein_coding retained_intron 15.58793 5.79382 26.38532 0.6528629 0.713627 2.185208 2.260585 0.1141186 4.291399 0.1141186 0.6261669 5.115015 0.1064708 0.0251000 0.1638000 0.1387 0.07977877 0.03567196 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100916 E001 18.707707 0.0009647378 3.487256e-04 1.575039e-03 14 35826338 35826440 103 + 1.274 0.891 -1.391
ENSG00000100916 E002 18.114445 0.0009918982 6.165593e-04 2.594117e-03 14 35826441 35826445 5 + 1.259 0.891 -1.340
ENSG00000100916 E003 24.170561 0.0008221393 4.612786e-06 3.310522e-05 14 35826446 35826456 11 + 1.388 0.928 -1.650
ENSG00000100916 E004 136.262839 0.0009718617 3.772757e-05 2.188352e-04 14 35826457 35826658 202 + 2.079 1.938 -0.474
ENSG00000100916 E005 2.126950 0.3275976128 7.135898e-01 8.100440e-01 14 35829795 35829907 113 + 0.411 0.494 0.429
ENSG00000100916 E006 134.465013 0.0073339976 4.102784e-02 9.293369e-02 14 35831410 35831500 91 + 2.063 1.979 -0.282
ENSG00000100916 E007 95.646982 0.0024342379 2.190810e-01 3.492711e-01 14 35832978 35833000 23 + 1.906 1.872 -0.112
ENSG00000100916 E008 150.683213 0.0042133509 1.235726e-01 2.250541e-01 14 35833001 35833105 105 + 2.106 2.060 -0.152
ENSG00000100916 E009 101.667302 0.0033442791 8.036506e-01 8.750456e-01 14 35834844 35834923 80 + 1.919 1.958 0.129
ENSG00000100916 E010 1.245984 0.0139849277 2.468001e-01 3.821895e-01 14 35858577 35858681 105 + 0.251 0.482 1.382
ENSG00000100916 E011 126.585938 0.0003340851 6.747526e-01 7.802116e-01 14 35862590 35862686 97 + 2.013 2.055 0.142
ENSG00000100916 E012 107.582325 0.0008808979 7.843800e-01 8.616874e-01 14 35863870 35863953 84 + 1.945 1.982 0.125
ENSG00000100916 E013 112.052403 0.0020003417 2.936629e-01 4.347229e-01 14 35864935 35864999 65 + 1.976 1.953 -0.076
ENSG00000100916 E014 112.465472 0.0052996517 3.087498e-01 4.510222e-01 14 35865722 35865761 40 + 1.978 1.953 -0.082
ENSG00000100916 E015 7.161296 0.0023052208 1.680352e-03 6.219963e-03 14 35865762 35866051 290 + 0.913 0.372 -2.404
ENSG00000100916 E016 5.241300 0.0031773567 2.970900e-01 4.383942e-01 14 35867777 35867905 129 + 0.676 0.852 0.705
ENSG00000100916 E017 157.577836 0.0003614995 2.695987e-01 4.081043e-01 14 35867906 35868032 127 + 2.120 2.109 -0.037
ENSG00000100916 E018 485.181433 0.0031964488 4.764636e-10 7.485247e-09 14 35870360 35871963 1604 + 2.556 2.742 0.622
ENSG00000100916 E019 0.147249 0.0445771922 1.000000e+00   14 35931723 35932325 603 + 0.063 0.001 -7.055