ENSG00000100889

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216780 ENSG00000100889 HEK293_OSMI2_2hA HEK293_TMG_2hB PCK2 protein_coding protein_coding 33.81804 41.79898 51.5793 2.007405 0.4770962 0.3032592 24.150333 27.943898 39.887987 0.98024841 0.5675515 0.5132665 0.69310000 0.66940000 0.77363333 0.104233333 0.00495910 0.0049591 FALSE TRUE
ENST00000396973 ENSG00000100889 HEK293_OSMI2_2hA HEK293_TMG_2hB PCK2 protein_coding protein_coding 33.81804 41.79898 51.5793 2.007405 0.4770962 0.3032592 1.734535 2.000707 2.191833 0.09467932 0.2297120 0.1310028 0.05221667 0.04803333 0.04250000 -0.005533333 0.76755452 0.0049591 FALSE TRUE
MSTRG.9241.5 ENSG00000100889 HEK293_OSMI2_2hA HEK293_TMG_2hB PCK2 protein_coding   33.81804 41.79898 51.5793 2.007405 0.4770962 0.3032592 4.175987 6.815847 5.084129 0.16103066 0.9344984 -0.4221727 0.12960000 0.16346667 0.09833333 -0.065133333 0.06059697 0.0049591 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100889 E001 0.0000000       14 24094053 24094132 80 +      
ENSG00000100889 E002 0.0000000       14 24094133 24094133 1 +      
ENSG00000100889 E003 0.0000000       14 24094134 24094149 16 +      
ENSG00000100889 E004 0.0000000       14 24094150 24094158 9 +      
ENSG00000100889 E005 0.9170165 0.0186240455 5.697568e-01 6.962836e-01 14 24094159 24094193 35 + 0.211 0.305 0.700
ENSG00000100889 E006 6.3248505 0.0472058469 2.675825e-01 4.059053e-01 14 24094194 24094254 61 + 0.778 0.927 0.575
ENSG00000100889 E007 0.9169173 0.0406787913 5.094796e-01 6.448123e-01 14 24094255 24094280 26 + 0.353 0.224 -0.892
ENSG00000100889 E008 4.9265376 0.0140755713 6.995308e-01 7.992208e-01 14 24094281 24094300 20 + 0.801 0.733 -0.273
ENSG00000100889 E009 8.8795023 0.0361052579 5.830163e-01 7.072194e-01 14 24094301 24094310 10 + 0.961 1.021 0.225
ENSG00000100889 E010 9.0267513 0.0379862211 6.583840e-01 7.675534e-01 14 24094311 24094312 2 + 0.975 1.021 0.171
ENSG00000100889 E011 10.7356985 0.0042744848 4.293267e-01 5.720663e-01 14 24094313 24094314 2 + 1.030 1.097 0.245
ENSG00000100889 E012 125.9814335 0.0125916442 1.963283e-03 7.111084e-03 14 24094315 24094349 35 + 2.196 1.992 -0.684
ENSG00000100889 E013 147.0346281 0.0134801521 2.700858e-03 9.376730e-03 14 24094350 24094356 7 + 2.261 2.063 -0.664
ENSG00000100889 E014 200.1174548 0.0107823904 1.109061e-02 3.138427e-02 14 24094357 24094376 20 + 2.374 2.223 -0.503
ENSG00000100889 E015 258.8356205 0.0083983272 1.870897e-02 4.869695e-02 14 24094377 24094434 58 + 2.473 2.350 -0.413
ENSG00000100889 E016 4.1225382 0.0062108368 5.027595e-01 6.387616e-01 14 24094435 24094736 302 + 0.648 0.733 0.354
ENSG00000100889 E017 1.6168542 0.0248894531 6.231328e-01 7.397934e-01 14 24094737 24094765 29 + 0.353 0.431 0.441
ENSG00000100889 E018 3.0873052 0.0051873360 9.891842e-01 9.972882e-01 14 24094766 24094845 80 + 0.616 0.607 -0.042
ENSG00000100889 E019 0.4439371 0.0215573264 1.023049e-01 1.940309e-01 14 24095143 24095378 236 + 0.288 0.000 -11.416
ENSG00000100889 E020 507.2817625 0.0019260931 3.699082e-05 2.149434e-04 14 24096892 24097137 246 + 2.761 2.645 -0.386
ENSG00000100889 E021 243.3105304 0.0001746554 1.755958e-04 8.593891e-04 14 24098203 24098254 52 + 2.436 2.335 -0.336
ENSG00000100889 E022 246.7706113 0.0007746869 1.198860e-01 2.198139e-01 14 24098255 24098387 133 + 2.421 2.367 -0.181
ENSG00000100889 E023 1.3703297 0.0431171835 6.588892e-01 7.678679e-01 14 24098388 24098474 87 + 0.353 0.427 0.419
ENSG00000100889 E024 113.2436247 0.0014192715 3.907480e-01 5.347110e-01 14 24098475 24098511 37 + 2.045 2.062 0.058
ENSG00000100889 E025 291.6350000 0.0018402282 9.104211e-01 9.470351e-01 14 24098512 24098678 167 + 2.471 2.460 -0.035
ENSG00000100889 E026 365.0907084 0.0009147657 8.214738e-01 8.874300e-01 14 24099049 24099236 188 + 2.573 2.554 -0.063
ENSG00000100889 E027 0.3030308 0.3856669817 3.284558e-01   14 24099237 24099247 11 + 0.212 0.000 -10.838
ENSG00000100889 E028 378.8196763 0.0011670331 4.355758e-01 5.777962e-01 14 24099558 24099720 163 + 2.576 2.580 0.015
ENSG00000100889 E029 3.8644752 0.0511945384 6.212768e-01 7.382522e-01 14 24099721 24099994 274 + 0.731 0.642 -0.373
ENSG00000100889 E030 366.0789313 0.0001589135 2.238025e-02 5.646612e-02 14 24099995 24100148 154 + 2.548 2.576 0.092
ENSG00000100889 E031 272.8822753 0.0001721379 2.949231e-04 1.359258e-03 14 24100149 24100213 65 + 2.401 2.464 0.212
ENSG00000100889 E032 40.1310792 0.0005749707 7.455758e-04 3.062799e-03 14 24100214 24100562 349 + 1.514 1.687 0.591
ENSG00000100889 E033 4.2362994 0.0039760965 3.914052e-02 8.944577e-02 14 24102675 24102752 78 + 0.545 0.829 1.198
ENSG00000100889 E034 401.1336786 0.0001615141 1.340628e-04 6.770040e-04 14 24102753 24102890 138 + 2.573 2.627 0.178
ENSG00000100889 E035 254.6742795 0.0032365807 5.125654e-01 6.474633e-01 14 24103160 24103255 96 + 2.401 2.411 0.032
ENSG00000100889 E036 705.9868910 0.0008907978 1.380810e-06 1.112345e-05 14 24103510 24103841 332 + 2.808 2.881 0.244
ENSG00000100889 E037 435.8413474 0.0017990210 3.785272e-04 1.691518e-03 14 24103842 24104130 289 + 2.596 2.674 0.258
ENSG00000100889 E038 0.2214452 0.0387838866 4.381754e-01   14 24110294 24110598 305 + 0.000 0.127 9.780