ENSG00000100796

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000554308 ENSG00000100796 HEK293_OSMI2_2hA HEK293_TMG_2hB PPP4R3A protein_coding nonsense_mediated_decay 39.44954 22.81051 54.28803 1.924318 2.259571 1.250569 2.275122 0.4039911 4.052048 0.1023438 0.1394998 3.2945356 0.05142917 0.01786667 0.07476667 0.056900000 3.010584e-08 1.898617e-28 TRUE TRUE
ENST00000554684 ENSG00000100796 HEK293_OSMI2_2hA HEK293_TMG_2hB PPP4R3A protein_coding protein_coding 39.44954 22.81051 54.28803 1.924318 2.259571 1.250569 9.533568 7.3150815 13.284840 0.6346266 0.9982192 0.8599497 0.25192917 0.32853333 0.24633333 -0.082200000 5.043265e-01 1.898617e-28 FALSE TRUE
ENST00000554943 ENSG00000100796 HEK293_OSMI2_2hA HEK293_TMG_2hB PPP4R3A protein_coding protein_coding 39.44954 22.81051 54.28803 1.924318 2.259571 1.250569 3.029153 2.7510890 4.128376 0.2903820 1.3080553 0.5838274 0.08920000 0.12203333 0.07436667 -0.047666667 4.203163e-01 1.898617e-28 FALSE TRUE
ENST00000555718 ENSG00000100796 HEK293_OSMI2_2hA HEK293_TMG_2hB PPP4R3A protein_coding processed_transcript 39.44954 22.81051 54.28803 1.924318 2.259571 1.250569 2.342280 1.4821629 3.276574 0.1492804 1.6536047 1.1391794 0.06099167 0.06586667 0.05790000 -0.007966667 8.552444e-01 1.898617e-28 FALSE FALSE
ENST00000557382 ENSG00000100796 HEK293_OSMI2_2hA HEK293_TMG_2hB PPP4R3A protein_coding retained_intron 39.44954 22.81051 54.28803 1.924318 2.259571 1.250569 11.208944 10.6174686 13.445458 2.4344544 0.1516946 0.3403935 0.33355833 0.45403333 0.24850000 -0.205533333 3.343758e-02 1.898617e-28   FALSE
MSTRG.10089.11 ENSG00000100796 HEK293_OSMI2_2hA HEK293_TMG_2hB PPP4R3A protein_coding   39.44954 22.81051 54.28803 1.924318 2.259571 1.250569 2.503540 0.0000000 5.351856 0.0000000 0.6269428 9.0665887 0.04517083 0.00000000 0.09793333 0.097933333 1.898617e-28 1.898617e-28 FALSE TRUE
MSTRG.10089.4 ENSG00000100796 HEK293_OSMI2_2hA HEK293_TMG_2hB PPP4R3A protein_coding   39.44954 22.81051 54.28803 1.924318 2.259571 1.250569 6.072744 0.1294036 6.619615 0.1294036 1.1430294 5.5715849 0.11628333 0.00660000 0.12060000 0.114000000 1.183262e-02 1.898617e-28 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000100796 E001 2.175586 0.0279594078 1.692180e-01 2.871718e-01 14 91457508 91457610 103 - 0.396 0.649 1.215
ENSG00000100796 E002 175.859817 0.0019846569 2.836012e-34 6.624833e-32 14 91457611 91458138 528 - 2.026 2.460 1.449
ENSG00000100796 E003 235.667377 0.0061307023 1.213294e-13 3.407783e-12 14 91458139 91458329 191 - 2.201 2.549 1.159
ENSG00000100796 E004 623.457834 0.0084932297 2.381713e-05 1.451811e-04 14 91458330 91458858 529 - 2.692 2.897 0.682
ENSG00000100796 E005 142.732107 0.0041554144 2.998485e-02 7.193015e-02 14 91458859 91458869 11 - 2.093 2.208 0.387
ENSG00000100796 E006 344.855195 0.0012283479 2.490961e-06 1.898862e-05 14 91461381 91461607 227 - 2.464 2.603 0.463
ENSG00000100796 E007 2.063916 0.0079567916 1.511403e-01 2.632249e-01 14 91461608 91461684 77 - 0.551 0.300 -1.364
ENSG00000100796 E008 117.487588 0.0021072304 3.400192e-03 1.143824e-02 14 91462049 91462053 5 - 2.000 2.137 0.457
ENSG00000100796 E009 316.621364 0.0024081607 2.563900e-01 3.933458e-01 14 91462054 91462221 168 - 2.460 2.514 0.182
ENSG00000100796 E010 140.705127 0.0008665114 8.709694e-01 9.210403e-01 14 91462222 91462239 18 - 2.119 2.145 0.087
ENSG00000100796 E011 261.401580 0.0028431307 9.918423e-01 9.989874e-01 14 91462735 91462877 143 - 2.387 2.407 0.068
ENSG00000100796 E012 198.414101 0.0018888499 2.548818e-01 3.916632e-01 14 91465250 91465419 170 - 2.254 2.312 0.195
ENSG00000100796 E013 13.663455 0.0013206568 5.781558e-03 1.803763e-02 14 91466261 91466416 156 - 1.219 0.952 -0.968
ENSG00000100796 E014 2.030516 0.0620974171 5.584443e-01 6.866811e-01 14 91468964 91469003 40 - 0.496 0.397 -0.512
ENSG00000100796 E015 1.481251 0.0093379512 1.281200e-02 3.547131e-02 14 91470210 91470247 38 - 0.496 0.001 -10.696
ENSG00000100796 E016 179.310230 0.0002211804 1.618849e-01 2.775867e-01 14 91470837 91470995 159 - 2.238 2.219 -0.063
ENSG00000100796 E017 155.082948 0.0002384012 1.537371e-01 2.667470e-01 14 91473033 91473135 103 - 2.177 2.154 -0.076
ENSG00000100796 E018 157.533342 0.0002578052 2.457726e-02 6.100585e-02 14 91473239 91473348 110 - 2.192 2.144 -0.160
ENSG00000100796 E019 80.250667 0.0024054901 1.310598e-01 2.356263e-01 14 91473349 91473370 22 - 1.903 1.852 -0.174
ENSG00000100796 E020 29.894065 0.0116964489 2.857868e-14 8.784919e-13 14 91474641 91475810 1170 - 1.614 0.778 -3.005
ENSG00000100796 E021 33.309753 0.0038912172 1.343769e-03 5.122295e-03 14 91475811 91475819 9 - 1.576 1.359 -0.745
ENSG00000100796 E022 41.469790 0.0007089200 7.402513e-03 2.226572e-02 14 91475820 91475849 30 - 1.650 1.509 -0.480
ENSG00000100796 E023 159.237555 0.0002677643 2.377771e-02 5.935938e-02 14 91475850 91475966 117 - 2.195 2.147 -0.162
ENSG00000100796 E024 137.447337 0.0002796119 8.057496e-04 3.279061e-03 14 91476408 91476479 72 - 2.145 2.055 -0.304
ENSG00000100796 E025 103.950519 0.0003610033 3.995741e-02 9.095817e-02 14 91476480 91476524 45 - 2.014 1.957 -0.192
ENSG00000100796 E026 133.852234 0.0002711896 1.237492e-02 3.443432e-02 14 91476909 91476986 78 - 2.125 2.062 -0.210
ENSG00000100796 E027 380.379148 0.0029501173 7.459510e-10 1.133356e-08 14 91481576 91482039 464 - 2.610 2.432 -0.591
ENSG00000100796 E028 198.823117 0.0115007355 6.799224e-05 3.704716e-04 14 91482040 91482193 154 - 2.338 2.126 -0.708
ENSG00000100796 E029 149.592087 0.0124084109 2.117744e-04 1.014310e-03 14 91485632 91485730 99 - 2.214 2.004 -0.705
ENSG00000100796 E030 109.276028 0.0131948335 1.195030e-03 4.623702e-03 14 91490747 91490802 56 - 2.075 1.879 -0.658
ENSG00000100796 E031 121.237434 0.0079464625 4.050588e-09 5.365860e-08 14 91509506 91509704 199 - 2.147 1.825 -1.081
ENSG00000100796 E032 95.481502 0.0045200242 3.570026e-11 6.786380e-10 14 91509705 91510074 370 - 2.049 1.707 -1.152
ENSG00000100796 E033 121.753590 0.0051754602 9.414587e-10 1.403056e-08 14 91510075 91510507 433 - 2.146 1.850 -0.992
ENSG00000100796 E034 4.124596 0.0045373046 5.494129e-02 1.178927e-01 14 91510529 91510554 26 - 0.771 0.475 -1.301